FastQCFastQC Report
Thu 2 Feb 2017
SRR4062949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470146
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27700.18841666065819312No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27610.1878044765621918No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25460.17318007871327065No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22790.15501861719856397No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT21880.1488287557834392No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21760.14801251032210405No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21620.1470602239505464No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20520.1395779738883077No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19530.13284394883229284No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19410.1320277033709577No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19290.1312114579096226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18610.12658606696205685No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18540.126109923776278No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17590.1196479805407082No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16630.11311801685002713No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16390.11148552592735686No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG16230.11039719864557669No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15580.10597586906334472No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15390.10468348041623077No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15190.10332307131400555No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG15050.10237078494244789No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC14890.10128245766066772No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14820.10080631447488889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5550.021.33249528
CTAGCGG5550.021.33249529
TAGCGGC5700.020.77111230
AGCGGCG6200.019.35407831
GGACCGT751.5072637E-619.202516
ACCGGTC601.137316E-418.6691078
AAGACGG5900.017.6294235
CGCAAGA6050.017.4544532
CAAGACG5900.017.357614
GCGCAAG6100.017.3119721
TCGCGTA3100.017.0339059
CGCGTAA3150.016.76295710
CGGTCCA7200.016.66771310
GCGGCGC7100.016.45005832
AGACGGA6250.016.386146
GTATCAA24200.016.3310491
GCGTAAC3450.016.23179811
TAGGACG4350.016.1854744
CCGGTCC7750.016.1047829
GTATTAC1203.1106538E-816.000461
Produced by FastQC (version 0.11.5)