##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1470146
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256988761660406	32.0	32.0	32.0	32.0	32.0
2	31.313832095587784	32.0	32.0	32.0	32.0	32.0
3	31.392488909264795	32.0	32.0	32.0	32.0	32.0
4	31.481960975304492	32.0	32.0	32.0	32.0	32.0
5	31.404177544271114	32.0	32.0	32.0	32.0	32.0
6	34.94644001344084	36.0	36.0	36.0	36.0	36.0
7	34.971243672397165	36.0	36.0	36.0	36.0	36.0
8	34.91091769116809	36.0	36.0	36.0	36.0	36.0
9	35.0198395261423	36.0	36.0	36.0	36.0	36.0
10	34.86508074708226	36.0	36.0	36.0	32.0	36.0
11	35.04741025721255	36.0	36.0	36.0	36.0	36.0
12	34.94007465925153	36.0	36.0	36.0	32.0	36.0
13	34.99808522418862	36.0	36.0	36.0	36.0	36.0
14	34.9335467361745	36.0	36.0	36.0	32.0	36.0
15	34.906996992135475	36.0	36.0	36.0	32.0	36.0
16	34.92442451293953	36.0	36.0	36.0	32.0	36.0
17	34.881375047104164	36.0	36.0	36.0	32.0	36.0
18	34.88572767602673	36.0	36.0	36.0	32.0	36.0
19	34.88530730961415	36.0	36.0	36.0	32.0	36.0
20	34.87335951667385	36.0	36.0	36.0	32.0	36.0
21	34.86461480696475	36.0	36.0	36.0	32.0	36.0
22	34.844539930047766	36.0	36.0	36.0	32.0	36.0
23	34.77983343150952	36.0	36.0	36.0	32.0	36.0
24	34.75945178233999	36.0	36.0	36.0	32.0	36.0
25	34.74047475556849	36.0	36.0	36.0	32.0	36.0
26	34.678092515981405	36.0	36.0	36.0	32.0	36.0
27	34.663398737268274	36.0	36.0	36.0	32.0	36.0
28	34.638162468217445	36.0	36.0	36.0	32.0	36.0
29	34.60500861819166	36.0	36.0	36.0	32.0	36.0
30	34.57788342110239	36.0	36.0	36.0	32.0	36.0
31	34.571169121978365	36.0	36.0	36.0	32.0	36.0
32	34.54928762177362	36.0	36.0	36.0	32.0	36.0
33	34.51960621598127	36.0	36.0	36.0	32.0	36.0
34	34.508084231089974	36.0	36.0	36.0	32.0	36.0
35	34.48976360171031	36.0	36.0	36.0	32.0	36.0
36	34.45560032813068	36.0	36.0	36.0	32.0	36.0
37	34.45454192984914	36.0	36.0	36.0	32.0	36.0
38	34.01745132796334	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	7.0
21	16.0
22	80.0
23	290.0
24	858.0
25	2174.0
26	4701.0
27	9146.0
28	15993.0
29	25520.0
30	39395.0
31	57195.0
32	83457.0
33	135963.0
34	337141.0
35	758205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.569944115456174	17.95740801839462	11.888611866026755	26.584036000122452
2	16.077450811007886	19.83109160586772	36.66812683910305	27.42333074402134
3	18.440073298842428	23.547457191326576	28.19821976864883	29.81424974118217
4	12.000593139175482	15.413660158460669	35.58284086456837	37.00290583779548
5	14.398259216960527	36.744996915266064	32.97067020010979	15.886073667663622
6	35.534866699951436	35.32740374739311	15.914176997868232	13.223552554787224
7	31.175759571033762	29.972199907627783	20.032935661185782	18.81910486015268
8	28.64949874362801	33.00755183639819	18.6028762551816	19.7400731647922
9	27.355722137570744	13.774828580757509	17.96963430561602	40.89981497605572
10	16.035419924591856	26.180352389885815	30.765243688836314	27.018983996686014
11	38.86848180844826	20.599587232786288	21.380158059640372	19.15177289912508
12	25.283237560402245	23.26894508293065	27.87801739149362	23.56979996517348
13	29.728972312254914	18.73162171078363	24.992976883232608	26.54642909372885
14	23.68689912430466	19.654034361213103	24.081961927590864	32.57710458689137
15	25.353672356351005	27.301097986186406	21.551124854266174	25.794104803196415
16	26.362347685195893	25.391695790758195	23.266532711717065	24.979423812328843
17	24.402270250709794	25.97000570011414	24.339759452462545	25.287964596713525
18	25.755537205148332	24.1184208915305	25.79417282365153	24.331869079669637
19	26.054895228092995	24.497294826500223	24.68088203484552	24.766927910561265
20	26.367245157963904	23.722201740507405	24.340711738834102	25.56984136269459
21	27.51315517391494	23.685774998911672	23.80664751208045	24.994422315092944
22	26.406170819885112	24.00361869067337	24.507514564889856	25.08269592455166
23	24.492397353732215	23.658534594523264	25.358229726843458	26.490838324901063
24	25.264837641975696	24.58490517268353	24.872767738714387	25.27748944662639
25	25.28616244395107	24.137839558573855	24.813759740542423	25.76223825693266
26	24.850814035350254	25.371715034911524	25.27825486601662	24.499216063721605
27	25.76621850134205	24.245549069409623	24.46131054007028	25.526921889178052
28	24.73611464439586	24.43566829416942	25.358569829119016	25.4696472323157
29	24.77536244699506	24.4657333353286	25.223753287088492	25.535150930587847
30	24.55205129286479	24.795020358522216	25.615891210804914	25.03703713780808
31	25.631739976845836	24.52021771987272	24.11882901426117	25.729213289020276
32	25.31973014925048	24.440089623751653	24.045366922741007	26.194813304256858
33	24.5194694948665	24.102912227765135	25.129408915849172	26.248209361519198
34	25.682823338634392	24.40866417349025	25.02615386499028	24.88235862288507
35	26.186106686002613	24.049040027317016	25.25749143282368	24.50736185385669
36	24.655102282358353	24.93072116646918	24.76461521508748	25.649561336084986
37	26.06951962594191	24.61027680244003	24.345813272967447	24.974390298650608
38	25.00692108601533	24.297467256631148	24.977876331926442	25.717735325427082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	20.0
2	30.0
3	30.0
4	155.5
5	281.0
6	281.0
7	440.5
8	600.0
9	635.0
10	670.0
11	670.0
12	926.5
13	1183.0
14	1609.0
15	2035.0
16	2035.0
17	3209.5
18	4384.0
19	4384.0
20	5320.5
21	6257.0
22	7080.0
23	7903.0
24	7903.0
25	9890.0
26	11877.0
27	11877.0
28	15556.5
29	19236.0
30	24335.0
31	29434.0
32	29434.0
33	39065.0
34	48696.0
35	48696.0
36	54707.5
37	60719.0
38	70153.0
39	79587.0
40	79587.0
41	83922.5
42	88258.0
43	100013.5
44	111769.0
45	111769.0
46	114285.0
47	116801.0
48	116801.0
49	126268.5
50	135736.0
51	138886.5
52	142037.0
53	142037.0
54	135642.5
55	129248.0
56	129248.0
57	126709.0
58	124170.0
59	111914.5
60	99659.0
61	99659.0
62	94784.5
63	89910.0
64	75471.5
65	61033.0
66	61033.0
67	51060.5
68	41088.0
69	41088.0
70	32604.0
71	24120.0
72	18629.5
73	13139.0
74	13139.0
75	9629.5
76	6120.0
77	6120.0
78	6077.0
79	6034.0
80	4695.5
81	3357.0
82	3357.0
83	3248.0
84	3139.0
85	3139.0
86	2010.0
87	881.0
88	717.0
89	553.0
90	553.0
91	338.5
92	124.0
93	80.0
94	36.0
95	36.0
96	24.5
97	13.0
98	13.0
99	16.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01027108872180042
2	0.0
3	0.0
4	1.3604091022252212E-4
5	2.0406136533378317E-4
6	2.7208182044504423E-4
7	4.761431857788274E-4
8	0.0027208182044504425
9	0.00448935003734323
10	8.842659164463938E-4
11	0.004625390947565752
12	1.3604091022252212E-4
13	6.802045511126106E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3604091022252212E-4
22	6.802045511126106E-5
23	0.0
24	0.0
25	1.3604091022252212E-4
26	6.802045511126106E-5
27	2.7208182044504423E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.802045511126106E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1470146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.670944609792045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.28156707301395	39.15930004514619
2	13.696212537940081	13.880000537478024
3	4.109377923473638	6.24678183423105
4	1.6930894707626167	3.431617711697388
5	0.8755479230909526	2.2182420157080065
6	0.5315518761744983	1.6160541404921427
7	0.3366423919343475	1.194059159650926
8	0.2596515461391876	1.0525431289812512
9	0.1676323062429836	0.7644678574004928
>10	0.8627846581228825	7.805747161541015
>50	0.07952962920288112	2.853895168202178
>100	0.08494112312111263	9.433307562529656
>500	0.01471948393160864	5.068770282618383
>1k	0.0067520568493617625	5.2752133943233845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2770	0.18841666065819312	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2761	0.1878044765621918	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2546	0.17318007871327065	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2279	0.15501861719856397	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2188	0.1488287557834392	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2176	0.14801251032210405	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2162	0.1470602239505464	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2052	0.1395779738883077	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1953	0.13284394883229284	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1941	0.1320277033709577	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1929	0.1312114579096226	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1861	0.12658606696205685	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1854	0.126109923776278	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1759	0.1196479805407082	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1663	0.11311801685002713	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1639	0.11148552592735686	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1623	0.11039719864557669	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1558	0.10597586906334472	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1539	0.10468348041623077	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1519	0.10332307131400555	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1505	0.10237078494244789	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1489	0.10128245766066772	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1482	0.10080631447488889	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3604091022252212E-4
2	0.0	0.0	0.0	0.0	1.3604091022252212E-4
3	0.0	0.0	0.0	0.0	1.3604091022252212E-4
4	0.0	0.0	0.0	0.0	1.3604091022252212E-4
5	0.0	0.0	0.0	0.0	1.3604091022252212E-4
6	0.0	0.0	0.0	0.0	2.0406136533378317E-4
7	0.0	0.0	0.0	6.802045511126106E-5	2.0406136533378317E-4
8	0.0	0.0	0.0	6.802045511126106E-5	2.0406136533378317E-4
9	0.0	0.0	0.0	6.802045511126106E-5	2.0406136533378317E-4
10	0.0	0.0	0.0	6.802045511126106E-5	2.0406136533378317E-4
11	0.0	0.0	0.0	6.802045511126106E-5	2.0406136533378317E-4
12	0.0	0.0	0.0	6.802045511126106E-5	6.121840960013495E-4
13	0.0	0.0	0.0	6.802045511126106E-5	6.121840960013495E-4
14	0.0	0.0	0.0	6.802045511126106E-5	6.121840960013495E-4
15	0.0	0.0	0.0	6.802045511126106E-5	7.482250062238716E-4
16	0.0	0.0	0.0	6.802045511126106E-5	7.482250062238716E-4
17	0.0	0.0	0.0	2.0406136533378317E-4	7.482250062238716E-4
18	0.0	0.0	0.0	2.0406136533378317E-4	7.482250062238716E-4
19	0.0	0.0	0.0	2.0406136533378317E-4	7.482250062238716E-4
20	0.0	0.0	0.0	2.7208182044504423E-4	0.001020306826668916
21	0.0	0.0	0.0	3.401022755563053E-4	0.001020306826668916
22	0.0	0.0	0.0	4.0812273066756635E-4	0.001020306826668916
23	0.0	0.0	0.0	5.441636408900885E-4	0.001020306826668916
24	0.0	0.0	0.0	6.802045511126106E-4	0.001088327281780177
25	0.0	0.0	0.0	7.482250062238716E-4	0.001088327281780177
26	0.0	0.0	0.0	8.842659164463938E-4	0.001088327281780177
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	555	0.0	21.332495	28
CTAGCGG	555	0.0	21.332495	29
TAGCGGC	570	0.0	20.771112	30
AGCGGCG	620	0.0	19.354078	31
GGACCGT	75	1.5072637E-6	19.20251	6
ACCGGTC	60	1.137316E-4	18.669107	8
AAGACGG	590	0.0	17.629423	5
CGCAAGA	605	0.0	17.454453	2
CAAGACG	590	0.0	17.35761	4
GCGCAAG	610	0.0	17.311972	1
TCGCGTA	310	0.0	17.033905	9
CGCGTAA	315	0.0	16.762957	10
CGGTCCA	720	0.0	16.667713	10
GCGGCGC	710	0.0	16.450058	32
AGACGGA	625	0.0	16.38614	6
GTATCAA	2420	0.0	16.331049	1
GCGTAAC	345	0.0	16.231798	11
TAGGACG	435	0.0	16.185474	4
CCGGTCC	775	0.0	16.104782	9
GTATTAC	120	3.1106538E-8	16.00046	1
>>END_MODULE