FastQCFastQC Report
Thu 2 Feb 2017
SRR4062948_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062948_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875677
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39940.4561042484843156No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA27790.3173544583219612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24560.28046871163682496No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC21020.24004284684878102No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20520.23433297894086516No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19340.22085769067818387No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17640.20144413979127007No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16040.18317256248593944No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15850.18100281268093144No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15030.17163862931194948No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14780.1687836953579916No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14720.16809851120904168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14720.16809851120904168No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14580.16649974819482527No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14040.16033309085427616No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13580.1550800123789936No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12140.13863559280419607No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11860.1354380667757632No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11830.13509547470128827No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11760.13429609319418004No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11710.13372510640338844No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG11710.13372510640338844No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11680.13338251432891352No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11620.13269733017996363No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11540.13178375131469708No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG11130.12710165963020612No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10880.12424672567624821No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10880.12424672567624821No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT10840.12378993624361495No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC10010.11431155551647468No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT9970.11385476608384142No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9680.11054304269725024No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG9550.10905847704119212No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG9320.10643193780355084No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC9300.10620354308723422No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9150.10449058271485946No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT9110.1040337932822262No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9110.1040337932822262No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8980.10254922762616808No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG8970.10243503026800978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT509.70238E-725.5907276
ACTGAGC1001.4551915E-1020.7972158
TAGAATA400.00449353519.9916134
TACAATA659.426549E-619.6930472
GTGTAGG751.4956786E-619.216091
TAAGCTG852.4035216E-718.816715
GTTCAAA4250.018.4625151
GTATAGA450.00880604317.7926751
ATCGTTT2300.017.39096329
CTAGCGG4700.017.3613629
TCTAGCG4700.017.36036928
GTATAGG652.0862854E-417.2452091
TATGGAC652.1055045E-417.2245273
GTCTTAG753.209117E-517.0809691
GTTATTC1601.8189894E-1216.9938413
TCTGTAC1051.1930206E-716.7558333
ACCGTCG2400.016.6644368
TAGCGGC4950.016.48546830
TCGTTCT3800.016.42166930
AGGCCCG4300.016.37270710
Produced by FastQC (version 0.11.5)