##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062948_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	875677
Sequences flagged as poor quality	0
Sequence length	38
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189705793346178	32.0	32.0	32.0	32.0	32.0
2	30.799328976323462	32.0	32.0	32.0	32.0	32.0
3	30.861539129153787	32.0	32.0	32.0	32.0	32.0
4	30.91631046607368	32.0	32.0	32.0	32.0	32.0
5	30.819873081056144	32.0	32.0	32.0	32.0	32.0
6	34.48403806426342	36.0	36.0	36.0	32.0	36.0
7	34.37784479893842	36.0	36.0	36.0	32.0	36.0
8	34.363331456690084	36.0	36.0	36.0	32.0	36.0
9	34.50559509956297	36.0	36.0	36.0	32.0	36.0
10	34.20623471896601	36.0	36.0	36.0	32.0	36.0
11	34.51018354941377	36.0	36.0	36.0	32.0	36.0
12	34.297809580473164	36.0	36.0	36.0	32.0	36.0
13	34.400305135341	36.0	36.0	36.0	32.0	36.0
14	34.282977627595564	36.0	36.0	36.0	32.0	36.0
15	34.22357558780235	36.0	36.0	36.0	32.0	36.0
16	34.234737237588746	36.0	36.0	36.0	32.0	36.0
17	34.12625431523267	36.0	36.0	36.0	32.0	36.0
18	34.17092032792913	36.0	36.0	36.0	32.0	36.0
19	34.1640707703868	36.0	36.0	36.0	32.0	36.0
20	34.13112825847887	36.0	36.0	36.0	32.0	36.0
21	34.09451201755898	36.0	36.0	36.0	32.0	36.0
22	34.07092797915213	36.0	36.0	36.0	32.0	36.0
23	34.014913033001896	36.0	36.0	36.0	32.0	36.0
24	33.98455709125625	36.0	36.0	36.0	32.0	36.0
25	33.95826200756672	36.0	36.0	36.0	32.0	36.0
26	33.893624018901946	36.0	36.0	36.0	32.0	36.0
27	33.89321290841258	36.0	36.0	36.0	32.0	36.0
28	33.880211539186256	36.0	36.0	36.0	32.0	36.0
29	33.8387133612051	36.0	36.0	36.0	32.0	36.0
30	33.803553136601735	36.0	36.0	36.0	32.0	36.0
31	33.81466796547129	36.0	36.0	36.0	32.0	36.0
32	33.77502321061304	36.0	36.0	36.0	32.0	36.0
33	33.70371038636392	36.0	36.0	36.0	27.0	36.0
34	33.703589337164274	36.0	36.0	36.0	27.0	36.0
35	33.63659203107995	36.0	36.0	36.0	27.0	36.0
36	33.58689334080945	36.0	36.0	36.0	27.0	36.0
37	33.58181041639783	36.0	36.0	36.0	27.0	36.0
38	32.931140135004114	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	5.0
10	9.0
11	10.0
12	4.0
13	5.0
14	115.0
15	222.0
16	307.0
17	381.0
18	461.0
19	611.0
20	885.0
21	1272.0
22	1970.0
23	2958.0
24	4299.0
25	6499.0
26	9618.0
27	13371.0
28	18644.0
29	25406.0
30	34140.0
31	45537.0
32	60931.0
33	89341.0
34	192387.0
35	366286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.46745487705176	16.291028008530052	11.004911069057979	25.23660604536021
2	17.472691956124315	19.170932358318097	36.03324954296161	27.323126142595978
3	19.678953830340063	23.624589555434998	27.046569397253233	29.649887216971706
4	12.834255134238804	14.159912566908128	34.160232332656875	38.84559996619619
5	15.488869145366882	35.74058382457398	31.885039409643312	16.885507620415815
6	35.94461415479488	33.78056927513061	16.1935649641705	14.081251605904017
7	31.868257359734244	29.50460044057341	19.75637135610505	18.8707708435873
8	30.45037308404726	30.268684464766572	18.731856832565544	20.549085618620627
9	28.075109972181494	13.76890539423257	17.895317446178723	40.260667187407215
10	17.24570701620354	24.849458644950797	29.030471420267613	28.874362918578058
11	39.25806142166066	20.78713918314582	20.378072567641027	19.5767268275525
12	26.399733226441185	22.920599463940462	26.510850808942855	24.1688165006755
13	30.15697525675231	18.10686287050912	24.961852636965023	26.774309235773547
14	25.22941699815781	19.090121049944667	23.31733267549044	32.36312927640708
15	26.524323033922837	25.544982392656884	22.098786161302225	25.831908412118054
16	27.508385936980694	24.57727682329172	22.442103628138373	25.47223361158921
17	24.951485032033013	24.8944892250512	24.18301250823812	25.971013234677663
18	26.798185055372258	22.74859270984298	25.750354891796544	24.702867342988217
19	26.42332365245218	24.12172556472124	24.791088259714098	24.663862523112485
20	26.81723495230786	22.211220443664804	24.689475751927787	26.28206885209955
21	28.6498055096834	22.771812494432528	23.132241994833386	25.446140001050683
22	26.88218216869231	22.860738734088162	24.137911343686543	26.119167753532977
23	24.662313797195264	22.748292296289023	25.07255099698375	27.516842909531963
24	25.382129922460432	24.364487358275284	24.480985160235875	25.77239755902841
25	26.03913963167537	23.086192166591285	24.142121590073234	26.732546611660112
26	25.30099014650605	24.448475032751702	24.87587475885952	25.374660061882732
27	26.544669206662462	23.601060768028944	24.198370930753136	25.65589909455546
28	25.286109267987396	23.14958309873953	25.274574676203454	26.289732957069624
29	24.687522841897522	23.992101271836855	25.65042303194211	25.669952854323512
30	24.90475624908639	24.173526348487062	25.745162439701797	25.176554962724744
31	25.61992437453676	24.271296927587077	23.58927447749109	26.51950422038507
32	25.333622653791902	24.099404418608636	23.600555038458683	26.966417889140775
33	24.632766942058147	23.48715512298967	24.608326614146613	27.27175132080557
34	25.983877857294598	23.961643097150873	24.65799265846321	25.396486387091322
35	27.170327662140675	23.363745180095393	24.750438588476086	24.715488569287842
36	24.964251287182087	25.018045676118945	23.949695964621338	26.068007072077627
37	26.68649332049626	24.503163013697066	24.160422298589864	24.64992136721681
38	25.269377784426965	24.062131336445	24.31703381226524	26.351457066862793
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	118.5
2	155.0
3	155.0
4	371.5
5	588.0
6	588.0
7	836.5
8	1085.0
9	1096.0
10	1107.0
11	1107.0
12	1382.0
13	1657.0
14	2141.0
15	2625.0
16	2625.0
17	3618.5
18	4612.0
19	4612.0
20	5232.0
21	5852.0
22	4999.5
23	4147.0
24	4147.0
25	4061.5
26	3976.0
27	3976.0
28	5480.0
29	6984.0
30	9400.0
31	11816.0
32	11816.0
33	16022.5
34	20229.0
35	20229.0
36	23462.0
37	26695.0
38	32257.0
39	37819.0
40	37819.0
41	41400.0
42	44981.0
43	54300.0
44	63619.0
45	63619.0
46	69380.0
47	75141.0
48	75141.0
49	79257.0
50	83373.0
51	84450.5
52	85528.0
53	85528.0
54	81704.0
55	77880.0
56	77880.0
57	77591.0
58	77302.0
59	70509.0
60	63716.0
61	63716.0
62	62017.5
63	60319.0
64	50262.5
65	40206.0
66	40206.0
67	33991.0
68	27776.0
69	27776.0
70	22527.0
71	17278.0
72	13648.0
73	10018.0
74	10018.0
75	7475.5
76	4933.0
77	4933.0
78	4911.5
79	4890.0
80	3906.5
81	2923.0
82	2923.0
83	3038.5
84	3154.0
85	3154.0
86	2133.5
87	1113.0
88	932.5
89	752.0
90	752.0
91	539.0
92	326.0
93	287.5
94	249.0
95	249.0
96	227.0
97	205.0
98	205.0
99	385.5
100	566.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12812943585363096
2	0.05447213984151691
3	0.01164813053214827
4	0.004111104893699389
5	3.425920744749491E-4
6	2.2839471631663273E-4
7	0.0
8	3.425920744749491E-4
9	1.1419735815831636E-4
10	4.5678943263326546E-4
11	0.0028549339539579092
12	0.003197526028432858
13	0.014274669769789547
14	0.010049367517931842
15	0.02169749805008011
16	0.011876525248464902
17	0.01987034031954705
18	0.006623446773182349
19	0.007765420354765513
20	0.006966038847657298
21	0.0062808546987074
22	0.008108012429240462
23	0.009820972801615207
24	0.01416047241163123
25	0.016558616932955874
26	0.017129603723747455
27	0.00970677544345689
28	0.0057098679079158185
29	0.010620354308723422
30	0.0033117233865911744
31	0.0052530784752825525
32	0.007080236205815615
33	0.008564801861873727
34	0.012675906755573117
35	0.01610182750032261
36	0.01381788033715628
37	0.010049367517931842
38	0.005481473191599186
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	875677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.647389911307776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.05069496828176	30.42370776636039
2	14.346033167516826	11.94949674016254
3	5.283445891124388	6.601251933088631
4	2.456045095407424	4.091514709127524
5	1.3503884990606467	2.8120078176062213
6	0.8186779518518965	2.0457479923540074
7	0.49983936185038924	1.4571903357201692
8	0.3688272735787389	1.228855461812666
9	0.2568055354283395	0.9625752238829595
>10	1.2632314075051503	9.619031984896127
>50	0.12581072063102505	3.7442014467017857
>100	0.15108242419221993	13.838238846703993
>500	0.020876844069954364	5.920835096464701
>1k	0.008240859501297776	5.30534464511833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3994	0.4561042484843156	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2779	0.3173544583219612	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2456	0.28046871163682496	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2102	0.24004284684878102	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2052	0.23433297894086516	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1934	0.22085769067818387	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1764	0.20144413979127007	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1604	0.18317256248593944	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1585	0.18100281268093144	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1503	0.17163862931194948	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1478	0.1687836953579916	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1472	0.16809851120904168	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1472	0.16809851120904168	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1458	0.16649974819482527	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1404	0.16033309085427616	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1358	0.1550800123789936	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1214	0.13863559280419607	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1186	0.1354380667757632	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1183	0.13509547470128827	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1176	0.13429609319418004	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1171	0.13372510640338844	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1171	0.13372510640338844	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1168	0.13338251432891352	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1162	0.13269733017996363	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1154	0.13178375131469708	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1113	0.12710165963020612	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1088	0.12424672567624821	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1088	0.12424672567624821	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1084	0.12378993624361495	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1001	0.11431155551647468	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	997	0.11385476608384142	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	968	0.11054304269725024	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	955	0.10905847704119212	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	932	0.10643193780355084	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	930	0.10620354308723422	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	915	0.10449058271485946	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT	911	0.1040337932822262	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	911	0.1040337932822262	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	898	0.10254922762616808	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	897	0.10243503026800978	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2839471631663273E-4
2	0.0	0.0	0.0	0.0	2.2839471631663273E-4
3	0.0	0.0	0.0	0.0	2.2839471631663273E-4
4	0.0	0.0	0.0	1.1419735815831636E-4	2.2839471631663273E-4
5	0.0	0.0	0.0	1.1419735815831636E-4	2.2839471631663273E-4
6	0.0	0.0	0.0	1.1419735815831636E-4	2.2839471631663273E-4
7	0.0	0.0	0.0	1.1419735815831636E-4	2.2839471631663273E-4
8	0.0	0.0	0.0	1.1419735815831636E-4	2.2839471631663273E-4
9	0.0	0.0	0.0	1.1419735815831636E-4	2.2839471631663273E-4
10	0.0	0.0	0.0	1.1419735815831636E-4	2.2839471631663273E-4
11	0.0	0.0	0.0	3.425920744749491E-4	3.425920744749491E-4
12	0.0	0.0	0.0	3.425920744749491E-4	3.425920744749491E-4
13	0.0	0.0	0.0	3.425920744749491E-4	3.425920744749491E-4
14	0.0	0.0	0.0	3.425920744749491E-4	3.425920744749491E-4
15	0.0	0.0	0.0	4.5678943263326546E-4	5.709867907915818E-4
16	0.0	0.0	0.0	4.5678943263326546E-4	5.709867907915818E-4
17	0.0	0.0	0.0	5.709867907915818E-4	6.851841489498982E-4
18	0.0	0.0	0.0	6.851841489498982E-4	6.851841489498982E-4
19	0.0	0.0	0.0	7.993815071082145E-4	6.851841489498982E-4
20	0.0	0.0	0.0	9.135788652665309E-4	0.0010277762234248472
21	0.0	0.0	0.0	0.0017129603723747455	0.0011419735815831637
22	0.0	0.0	0.0	0.0023981445213246437	0.0011419735815831637
23	0.0	0.0	0.0	0.0035401181029078074	0.0011419735815831637
24	0.0	0.0	0.0	0.005595670549757502	0.0011419735815831637
25	0.0	0.0	0.0	0.0069660388476572984	0.0011419735815831637
26	0.0	0.0	0.0	0.007651222996607196	0.0011419735815831637
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	50	9.70238E-7	25.590727	6
ACTGAGC	100	1.4551915E-10	20.797215	8
TAGAATA	40	0.004493535	19.991613	4
TACAATA	65	9.426549E-6	19.693047	2
GTGTAGG	75	1.4956786E-6	19.21609	1
TAAGCTG	85	2.4035216E-7	18.81671	5
GTTCAAA	425	0.0	18.462515	1
GTATAGA	45	0.008806043	17.792675	1
ATCGTTT	230	0.0	17.390963	29
CTAGCGG	470	0.0	17.36136	29
TCTAGCG	470	0.0	17.360369	28
GTATAGG	65	2.0862854E-4	17.245209	1
TATGGAC	65	2.1055045E-4	17.224527	3
GTCTTAG	75	3.209117E-5	17.080969	1
GTTATTC	160	1.8189894E-12	16.993841	3
TCTGTAC	105	1.1930206E-7	16.755833	3
ACCGTCG	240	0.0	16.664436	8
TAGCGGC	495	0.0	16.485468	30
TCGTTCT	380	0.0	16.421669	30
AGGCCCG	430	0.0	16.372707	10
>>END_MODULE