FastQCFastQC Report
Thu 2 Feb 2017
SRR4062948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875677
Sequences flagged as poor quality0
Sequence length38
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46100.5264498211098385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31220.35652415217026373No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA26960.30787607759482094No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC26430.30182361761243015No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22300.2546601086930455No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20510.23421878158270687No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20250.23124965027059063No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17520.20007377149337027No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17390.19858920583731213No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16540.18888243039385527No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15750.17986083909934827No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15320.17495035269854067No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14340.16375901159902567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14030.16021889349611787No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13950.15930531463085135No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13910.15884852519821807No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13500.15416643351372708No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13310.15199668370871908No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12990.14834236824765296No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12280.14023435581841248No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG12190.13920657959498767No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11700.13361090904523015No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11410.130299185658639No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10980.12538869925783136No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT10970.12527450189967307No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10890.12436092303440652No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG10660.12173438379676524No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10450.1193362392754406No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC10450.1193362392754406No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG10320.1178516736193825No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10240.11693809475411596No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10170.11613871324700775No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG10150.11591031853069111No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT10010.11431155551647468No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9980.11396896344199973No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT9910.11316958193489152No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9810.11202760835330836No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT9790.11179921363699173No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA9490.10837329289224223No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT9470.1081448981759256No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC9000.10277762234248472No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG8990.10266342498432642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC356.423696E-527.431854
CTAGCGG3900.020.92199529
TCTAGCG4050.020.14710628
CGTATGA400.004483985219.99896619
TAGCGGC4100.019.51118530
AAGACGG5000.018.8833355
CGCAAGA4950.018.1011072
GTTCAAA4250.018.0706841
CAAGACG5350.017.9460724
GTCGTAT450.00885127917.77685717
CCCGCAA450.00885127917.77685721
AGCGGCG4550.017.58150931
AACCGCT550.001362679217.457637
CTTAAAC550.001363200817.4566333
TATATGT550.001363200817.4566334
GAATATT550.001364244717.454641
CGGCATC3250.017.22987725
TACCGTC2750.016.8757087
GCGCAAG5500.016.8728181
ATCGTTT3250.016.73759529
Produced by FastQC (version 0.11.5)