FastQCFastQC Report
Thu 2 Feb 2017
SRR4062947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995469
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42010.42201213699271395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24740.24852607163055804No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17370.17449061698556156No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17010.17087423114130124No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16790.1686642175698088No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15700.15771460487468722No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15420.15490186032915138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15220.1528927570823401No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15010.15078319867318823No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14940.15008001253680425No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14830.14897500575105804No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14770.14837227477701467No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14510.14576044055615997No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14480.14545907506913827No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12880.12938624909464785No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12840.1289844284452856No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12340.12396167032825732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12290.1234593945165545No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12250.12305757386719225No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11640.11692980896441778No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11450.11502116087994704No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT11380.11431797474356307No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11330.11381569893186025No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11190.11240932665909234No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10840.10889339597717257No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT10700.10748702370440466No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10680.10728611337972353No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC10550.10598019626929618No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT10500.10547792045759335No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT10390.10437291367184713No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG10060.1010578933146085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGG350.002056106722.8766821
GGTCTAT453.5159528E-421.3515681
GTATTAG2700.018.3860741
GTAGGAC4900.018.2811223
TCTAGCG3750.017.919128
TGTAGGA5300.017.8149012
CTAGCGG3850.017.45454629
ATTAGCT1750.017.3670653
TAGGACG4800.017.328984
AGGACGT4900.017.3017775
TCGTTCT4450.017.25842730
TTCGTTC4400.017.0909129
ATCGTTT3100.017.03225929
CGTTTAT3100.017.03225931
TAGCGGC3950.017.0126630
CTTTCGT4350.016.9186927
CCTCTAT957.62142E-716.8565031
GGACGTG4950.016.803866
CGACTTT4550.016.52747324
CATCGTT3200.016.49917228
Produced by FastQC (version 0.11.5)