##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062947_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	995469
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.212194453066846	32.0	32.0	32.0	32.0	32.0
2	30.855609767858166	32.0	32.0	32.0	32.0	32.0
3	30.91567190942159	32.0	32.0	32.0	32.0	32.0
4	30.970029202315693	32.0	32.0	32.0	32.0	32.0
5	30.912917428870212	32.0	32.0	32.0	32.0	32.0
6	34.55565467131573	36.0	36.0	36.0	32.0	36.0
7	34.466644365620624	36.0	36.0	36.0	32.0	36.0
8	34.458977627630794	36.0	36.0	36.0	32.0	36.0
9	34.56078491645646	36.0	36.0	36.0	32.0	36.0
10	34.30924820361056	36.0	36.0	36.0	32.0	36.0
11	34.5667177983443	36.0	36.0	36.0	32.0	36.0
12	34.39069222647817	36.0	36.0	36.0	32.0	36.0
13	34.47335677956822	36.0	36.0	36.0	32.0	36.0
14	34.372767007310124	36.0	36.0	36.0	32.0	36.0
15	34.33463925044376	36.0	36.0	36.0	32.0	36.0
16	34.34532567061355	36.0	36.0	36.0	32.0	36.0
17	34.258079357569144	36.0	36.0	36.0	32.0	36.0
18	34.29743266741606	36.0	36.0	36.0	32.0	36.0
19	34.26652562761874	36.0	36.0	36.0	32.0	36.0
20	34.243576645782035	36.0	36.0	36.0	32.0	36.0
21	34.21055904302394	36.0	36.0	36.0	32.0	36.0
22	34.18578278178426	36.0	36.0	36.0	32.0	36.0
23	34.13360536591295	36.0	36.0	36.0	32.0	36.0
24	34.1285514666956	36.0	36.0	36.0	32.0	36.0
25	34.10145368665423	36.0	36.0	36.0	32.0	36.0
26	34.06250018835343	36.0	36.0	36.0	32.0	36.0
27	34.052308007582354	36.0	36.0	36.0	32.0	36.0
28	34.03450433916074	36.0	36.0	36.0	32.0	36.0
29	33.99879956081003	36.0	36.0	36.0	32.0	36.0
30	33.96064568560146	36.0	36.0	36.0	32.0	36.0
31	33.983012027496585	36.0	36.0	36.0	32.0	36.0
32	33.9287883399684	36.0	36.0	36.0	32.0	36.0
33	33.874121645174284	36.0	36.0	36.0	32.0	36.0
34	33.875310029744774	36.0	36.0	36.0	32.0	36.0
35	33.81843332137917	36.0	36.0	36.0	32.0	36.0
36	33.77910010256472	36.0	36.0	36.0	32.0	36.0
37	33.775355134112665	36.0	36.0	36.0	32.0	36.0
38	33.15811743007567	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	8.0
12	8.0
13	10.0
14	190.0
15	370.0
16	405.0
17	487.0
18	591.0
19	776.0
20	1111.0
21	1505.0
22	2227.0
23	3208.0
24	4700.0
25	6840.0
26	9946.0
27	14078.0
28	19317.0
29	26039.0
30	35088.0
31	46770.0
32	63683.0
33	95051.0
34	212549.0
35	450503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13783850112046	17.005218076995803	11.287360345028747	25.56958307685499
2	16.790244069139018	19.98888356184348	37.581099240845774	25.63977312817173
3	18.997825903495837	24.85412254509944	28.047753768500915	28.100297782903805
4	12.581433592882767	15.436042043592652	35.68923584775823	36.29328851576635
5	14.683273964153473	36.536657203761855	32.87033409478576	15.909734737298914
6	33.79392164071902	35.34254309037175	17.09591286894781	13.767622399961423
7	30.139863722526766	30.334244461655764	21.048068799731585	18.477823016085885
8	28.45126960512001	32.090164716660624	19.59904245946877	19.859523218750596
9	28.23046871468366	13.999459550140788	18.5764068522613	39.19366488291425
10	16.699231012642333	25.601502815267235	30.59695720090611	27.102308971184318
11	37.822427287165596	20.84158331299092	22.324620752076683	19.011368647766805
12	26.216363101199764	23.266532522575282	28.1158112887979	22.401293087427053
13	30.08802079304112	19.14736073253876	24.99133455838398	25.77328391603614
14	24.42941045275444	20.154032554597567	24.421976061259386	30.994580931388604
15	25.318037906320367	27.15326809633426	22.486367650915405	25.042326346429967
16	25.88028805774015	25.164061903479762	24.177882084399954	24.777767954380142
17	23.99471497540856	25.599413221604294	25.21147634047213	25.194395462515008
18	25.485324728555696	23.573259823028764	26.99826002394979	23.94315542446575
19	26.007140905291582	24.270940493795408	25.500204944383718	24.221713656529285
20	26.41782573097653	23.189624428002393	25.167091112751965	25.22545872826911
21	27.902969952873356	23.466676311001674	23.780217660585713	24.850136075539254
22	26.586692438447102	23.997090628528717	24.547821788941977	24.8683951440822
23	24.090948363335336	23.76825688331468	25.716059627598764	26.42473512575122
24	25.20585930435831	24.807101091084455	25.246749854321138	24.7402897502361
25	25.06686359023822	23.95474776702736	25.436095286895537	25.542293355838886
26	24.373874068484767	25.346960223533515	26.06604929030521	24.21311641767651
27	25.658490911027123	24.443379526654088	24.75562145990183	25.14250810241696
28	24.260431118402348	24.381988947279062	25.828225727865174	25.529354206453412
29	24.33818048107121	24.479636554339024	25.806490502952695	25.375692461637072
30	24.101458039913766	24.80797552052045	26.258983410451194	24.831583029114586
31	25.41440293829264	24.622968672142477	24.353531416263483	25.609096973301398
32	24.63477067556625	24.869449729857887	24.432038513083157	26.063741081492704
33	23.982933271179956	24.373635580934852	25.72687808862282	25.916553059262377
34	25.28414713662672	24.57695657896191	25.57932057865247	24.559575705758895
35	26.004077093026645	24.41161961465543	25.636455521205807	23.94784777111212
36	24.279832496754825	25.416346837813613	25.110516776446662	25.193303888984893
37	25.51046242008013	25.17651692030718	24.743512978062473	24.569507681550213
38	24.528707525911667	25.209840054410233	25.06577751077204	25.195674908906057
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	640.0
1	490.0
2	340.0
3	340.0
4	766.5
5	1193.0
6	1193.0
7	1362.5
8	1532.0
9	1450.0
10	1368.0
11	1368.0
12	1750.5
13	2133.0
14	2517.5
15	2902.0
16	2902.0
17	4108.5
18	5315.0
19	5315.0
20	6289.5
21	7264.0
22	6765.5
23	6267.0
24	6267.0
25	7346.0
26	8425.0
27	8425.0
28	11173.5
29	13922.0
30	18552.0
31	23182.0
32	23182.0
33	29947.0
34	36712.0
35	36712.0
36	39920.5
37	43129.0
38	48729.0
39	54329.0
40	54329.0
41	56215.0
42	58101.0
43	65771.0
44	73441.0
45	73441.0
46	75654.5
47	77868.0
48	77868.0
49	83108.5
50	88349.0
51	88882.5
52	89416.0
53	89416.0
54	85390.0
55	81364.0
56	81364.0
57	80363.5
58	79363.0
59	71440.5
60	63518.0
61	63518.0
62	62458.0
63	61398.0
64	50980.5
65	40563.0
66	40563.0
67	34086.0
68	27609.0
69	27609.0
70	22300.5
71	16992.0
72	13391.0
73	9790.0
74	9790.0
75	7340.5
76	4891.0
77	4891.0
78	4939.0
79	4987.0
80	3941.0
81	2895.0
82	2895.0
83	3053.0
84	3211.0
85	3211.0
86	2110.5
87	1010.0
88	896.0
89	782.0
90	782.0
91	558.0
92	334.0
93	286.5
94	239.0
95	239.0
96	225.0
97	211.0
98	211.0
99	347.5
100	484.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12386121516591676
2	0.05484851863794855
3	0.011351433344483856
4	0.0026118342208546926
5	3.013654870216953E-4
6	3.013654870216953E-4
7	0.0
8	2.0091032468113023E-4
9	2.0091032468113023E-4
10	4.0182064936226047E-4
11	0.0012054619480867813
12	0.002210013571492432
13	0.013661902078316855
14	0.009543240422353685
15	0.02270286668896771
16	0.011351433344483856
17	0.02049285311747528
18	0.005324123604049951
19	0.007735047500223513
20	0.0054245787663905155
21	0.006630040714477297
22	0.006529585552136732
23	0.009141419772991424
24	0.012958715941932899
25	0.01597237081214985
26	0.018282839545982848
27	0.008739599123629165
28	0.005826399415752776
29	0.010748702370440465
30	0.0023104687338329974
31	0.006127764902774471
32	0.006730495876817863
33	0.008237323311926338
34	0.012255529805548941
35	0.015269184675765896
36	0.01456599853938194
37	0.009744150747034814
38	0.006830951039158427
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	995469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.096124512985874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06649006227235	39.60924730964356
2	12.870132117994451	12.894874821632573
3	3.7959761612706733	5.704910832700254
4	1.4780498154524342	2.9617827036520374
5	0.7634977255900606	1.9124138563270596
6	0.4448423956581807	1.3370928024928286
7	0.27051495540125325	0.9486225621884201
8	0.1970917593562878	0.7898826653756833
9	0.13954864038968634	0.6291761458121655
>10	0.7295460537106424	6.832262659708883
>50	0.10387452749814974	3.6776927642242643
>100	0.118134823821348	12.919970316886161
>500	0.016072765105971863	5.292066858155029
>1k	0.0062281964785640955	4.490003701201103
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4201	0.42201213699271395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2474	0.24852607163055804	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1737	0.17449061698556156	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1701	0.17087423114130124	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1679	0.1686642175698088	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1570	0.15771460487468722	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1542	0.15490186032915138	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1522	0.1528927570823401	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1501	0.15078319867318823	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1494	0.15008001253680425	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1483	0.14897500575105804	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1477	0.14837227477701467	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1451	0.14576044055615997	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1448	0.14545907506913827	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1288	0.12938624909464785	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1284	0.1289844284452856	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1234	0.12396167032825732	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1229	0.1234593945165545	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1225	0.12305757386719225	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1164	0.11692980896441778	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1145	0.11502116087994704	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1138	0.11431797474356307	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1133	0.11381569893186025	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1119	0.11240932665909234	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1084	0.10889339597717257	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1070	0.10748702370440466	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1068	0.10728611337972353	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1055	0.10598019626929618	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1050	0.10547792045759335	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1039	0.10437291367184713	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1006	0.1010578933146085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.004551623405651E-4
7	0.0	0.0	0.0	0.0	1.004551623405651E-4
8	0.0	0.0	0.0	0.0	1.004551623405651E-4
9	0.0	0.0	0.0	0.0	1.004551623405651E-4
10	0.0	0.0	0.0	0.0	1.004551623405651E-4
11	0.0	0.0	0.0	0.0	1.004551623405651E-4
12	0.0	0.0	0.0	0.0	2.009103246811302E-4
13	0.0	0.0	0.0	0.0	2.009103246811302E-4
14	0.0	0.0	0.0	0.0	2.009103246811302E-4
15	0.0	0.0	0.0	1.004551623405651E-4	2.009103246811302E-4
16	0.0	0.0	0.0	2.009103246811302E-4	3.013654870216953E-4
17	0.0	0.0	0.0	2.009103246811302E-4	3.013654870216953E-4
18	0.0	0.0	0.0	2.009103246811302E-4	3.013654870216953E-4
19	0.0	0.0	0.0	2.009103246811302E-4	3.013654870216953E-4
20	0.0	0.0	0.0	2.009103246811302E-4	4.018206493622604E-4
21	0.0	0.0	0.0	8.036412987245208E-4	4.018206493622604E-4
22	0.0	0.0	0.0	0.0013059171104273463	4.018206493622604E-4
23	0.0	0.0	0.0	0.0029131997078763877	4.018206493622604E-4
24	0.0	0.0	0.0	0.0047213926300065595	4.018206493622604E-4
25	0.0	0.0	0.0	0.006027309740433906	4.018206493622604E-4
26	0.0	0.0	0.0	0.007132316526180123	4.018206493622604E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATGG	35	0.0020561067	22.876682	1
GGTCTAT	45	3.5159528E-4	21.351568	1
GTATTAG	270	0.0	18.386074	1
GTAGGAC	490	0.0	18.281122	3
TCTAGCG	375	0.0	17.9191	28
TGTAGGA	530	0.0	17.814901	2
CTAGCGG	385	0.0	17.454546	29
ATTAGCT	175	0.0	17.367065	3
TAGGACG	480	0.0	17.32898	4
AGGACGT	490	0.0	17.301777	5
TCGTTCT	445	0.0	17.258427	30
TTCGTTC	440	0.0	17.09091	29
ATCGTTT	310	0.0	17.032259	29
CGTTTAT	310	0.0	17.032259	31
TAGCGGC	395	0.0	17.01266	30
CTTTCGT	435	0.0	16.91869	27
CCTCTAT	95	7.62142E-7	16.856503	1
GGACGTG	495	0.0	16.80386	6
CGACTTT	455	0.0	16.527473	24
CATCGTT	320	0.0	16.499172	28
>>END_MODULE