FastQCFastQC Report
Thu 2 Feb 2017
SRR4062947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995469
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48650.48871436478684926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33240.3339129596200384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20230.2032207934149632No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19210.19297436685622554No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18080.1816229335117417No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16770.16846330724512767No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16240.16313918364107774No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16210.16283781815405604No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16140.16213463201767206No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15620.1569109635759627No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14940.15008001253680425No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14770.14837227477701467No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14610.14676499217956562No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14520.14586089571850053No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14340.14405270279637034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14230.14294769601062413No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14220.14284724084828357No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13240.13300263493890818No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13220.13280172461422707No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12720.1277789664971988No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12720.1277789664971988No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT11840.11893891221122908No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG11600.11652798831505552No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT11350.11401660925654139No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT10990.11040022341228105No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA10880.10929521662653484No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10880.10929521662653484No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC10720.1076879340290858No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10540.10587974110695562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGA350.00206649822.8568572
GTATAGA400.004482087720.0007531
ATGGGCG1154.5474735E-1119.4789945
CTAGCGG3650.018.8490829
TCTAGCG3700.018.5943628
ACCGTCC701.786289E-518.2864048
GTATTAG1700.017.8830281
ACCGGTC450.00884754317.7784488
GGTATAC450.00884754317.7784481
CATTACG450.00885010317.7775552
TGTAGGA5850.017.7775542
ATTAGAG2800.017.7149523
GTAGGAC6200.017.5490473
ACACCGT652.0994437E-417.2314196
GGCCCGT652.0994437E-417.2314196
AATTAGA2550.016.9409642
TAGGACG5400.016.5932184
TAGCGGC4300.016.37188730
AGCGGCG4250.016.18803431
CTGTAGG6150.016.1306881
Produced by FastQC (version 0.11.5)