##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062947_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	995469
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16436674572488	32.0	32.0	32.0	32.0	32.0
2	31.317113842821826	32.0	32.0	32.0	32.0	32.0
3	31.390155795911273	32.0	32.0	32.0	32.0	32.0
4	31.49040201151417	32.0	32.0	32.0	32.0	32.0
5	31.41865994822541	32.0	32.0	32.0	32.0	32.0
6	34.953922221586005	36.0	36.0	36.0	36.0	36.0
7	34.97054755095337	36.0	36.0	36.0	36.0	36.0
8	34.92402977892832	36.0	36.0	36.0	36.0	36.0
9	35.00878379939506	36.0	36.0	36.0	36.0	36.0
10	34.86404900604639	36.0	36.0	36.0	32.0	36.0
11	35.03520049343576	36.0	36.0	36.0	36.0	36.0
12	34.924286944143915	36.0	36.0	36.0	32.0	36.0
13	34.97353508748138	36.0	36.0	36.0	36.0	36.0
14	34.914839136125785	36.0	36.0	36.0	32.0	36.0
15	34.89968246123184	36.0	36.0	36.0	32.0	36.0
16	34.89897324778572	36.0	36.0	36.0	32.0	36.0
17	34.86526250440747	36.0	36.0	36.0	32.0	36.0
18	34.862128303342445	36.0	36.0	36.0	32.0	36.0
19	34.848120835505675	36.0	36.0	36.0	32.0	36.0
20	34.83109469004057	36.0	36.0	36.0	32.0	36.0
21	34.817956159358054	36.0	36.0	36.0	32.0	36.0
22	34.802330358855976	36.0	36.0	36.0	32.0	36.0
23	34.74669427174528	36.0	36.0	36.0	32.0	36.0
24	34.71579828201581	36.0	36.0	36.0	32.0	36.0
25	34.68584958446722	36.0	36.0	36.0	32.0	36.0
26	34.63186297112215	36.0	36.0	36.0	32.0	36.0
27	34.59620239304288	36.0	36.0	36.0	32.0	36.0
28	34.558882295681734	36.0	36.0	36.0	32.0	36.0
29	34.52857396865196	36.0	36.0	36.0	32.0	36.0
30	34.49371703187141	36.0	36.0	36.0	32.0	36.0
31	34.496537812829935	36.0	36.0	36.0	32.0	36.0
32	34.448221893398994	36.0	36.0	36.0	32.0	36.0
33	34.40769325815269	36.0	36.0	36.0	32.0	36.0
34	34.390194973424585	36.0	36.0	36.0	32.0	36.0
35	34.348778314543196	36.0	36.0	36.0	32.0	36.0
36	34.30513556926434	36.0	36.0	36.0	32.0	36.0
37	34.28416655867737	36.0	36.0	36.0	32.0	36.0
38	33.85202552766585	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	22.0
22	89.0
23	249.0
24	684.0
25	1636.0
26	3626.0
27	7064.0
28	11905.0
29	18962.0
30	28697.0
31	40899.0
32	58060.0
33	91390.0
34	222937.0
35	509244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44704257189502	18.2029888233078	11.996182343337939	26.35378626145925
2	15.67190942158922	21.051283364926483	36.86312682765611	26.413680385828187
3	18.01422244188418	25.47362097664518	28.534087952512838	27.978068628957807
4	12.021770642782448	16.18855032150675	35.906793682173934	35.88288535353688
5	14.100905302832437	36.89942820864157	32.69165859676052	16.30800789176548
6	33.787022787271916	35.894875576110934	16.633482944705406	13.684618691911743
7	29.686275691640045	30.684306752657065	21.100797621200247	18.52861993450264
8	28.308001406399114	32.20332512933849	19.520317444371894	19.9683560198905
9	28.10754824833262	13.648268123199886	18.398610439983806	39.84557318848369
10	16.459410507832004	25.904826578882073	30.560028609687716	27.07573430359821
11	37.856278907163556	20.66819498748277	22.400916992489663	19.074609112864014
12	25.98921305965164	23.484750312166298	28.041423940417676	22.48461268776439
13	30.108451502208005	19.082883628604836	25.035259797401828	25.773405071785334
14	24.298018620387595	20.026058095287844	24.535093041664823	31.140830242659735
15	25.233131318001867	27.185979673902448	22.480860780195062	25.100028227900616
16	25.78714153831008	25.16863910377922	24.14379553758078	24.900423820329916
17	23.983167732998215	25.618477320740276	25.178081889039237	25.220273057222276
18	25.491200630054777	23.492243354639875	27.130427969128117	23.88612804617723
19	26.139035971989085	24.119585843456704	25.51691715161396	24.224461032940255
20	26.334923538553184	23.046021523523084	25.326454163816255	25.29260077410748
21	27.872892073987238	23.47707462512645	23.906922264781727	24.74311103610459
22	26.57089271489117	23.86332472432592	24.746928332273534	24.818854228509377
23	23.909304787908376	23.646412835797506	25.944532510402162	26.499749865891953
24	25.221076698521	24.620656193211442	25.420681106091703	24.73758600217586
25	25.167935917642843	23.810183943447765	25.52354719232844	25.49833294658096
26	24.527232892234714	25.127452487219593	26.109703064585638	24.235611555960055
27	25.709288787496142	24.253090754207314	24.909565240102907	25.128055218193634
28	24.28774778521481	24.052582250175544	26.098853907052856	25.56081605755679
29	24.440540087134806	24.241136589888786	25.931796972080495	25.38652635089591
30	24.051879064039163	24.525826519961946	26.519560126935144	24.902734289063748
31	25.306363131348135	24.26825948372074	24.71829861100647	25.707078773924653
32	24.780982632306984	24.52974427129323	24.602373353665456	26.08689974273433
33	23.945095226471143	24.062527311247262	26.05304635302556	25.939331109256038
34	25.231021759592714	24.22024191612195	25.94103884701583	24.607697477269507
35	26.1457664678659	24.056901822156192	25.755397707010463	24.041934002967448
36	24.455809271810576	25.076320809588243	25.25312189530764	25.21474802329354
37	25.718329752106794	24.876214126205838	24.837337978380038	24.568118143307323
38	24.6211832022727	24.692405983919123	25.494239895205066	25.192170918603107
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	293.0
1	223.0
2	153.0
3	153.0
4	577.5
5	1002.0
6	1002.0
7	1273.0
8	1544.0
9	1517.5
10	1491.0
11	1491.0
12	1842.0
13	2193.0
14	2566.0
15	2939.0
16	2939.0
17	4536.0
18	6133.0
19	6133.0
20	6905.0
21	7677.0
22	7102.0
23	6527.0
24	6527.0
25	7281.0
26	8035.0
27	8035.0
28	10789.5
29	13544.0
30	18001.5
31	22459.0
32	22459.0
33	29716.5
34	36974.0
35	36974.0
36	40147.0
37	43320.0
38	48953.0
39	54586.0
40	54586.0
41	56337.0
42	58088.0
43	65238.0
44	72388.0
45	72388.0
46	75805.0
47	79222.0
48	79222.0
49	84245.0
50	89268.0
51	89367.0
52	89466.0
53	89466.0
54	85134.5
55	80803.0
56	80803.0
57	80511.5
58	80220.0
59	72160.5
60	64101.0
61	64101.0
62	63014.5
63	61928.0
64	51224.5
65	40521.0
66	40521.0
67	34009.5
68	27498.0
69	27498.0
70	22339.0
71	17180.0
72	13444.0
73	9708.0
74	9708.0
75	7218.0
76	4728.0
77	4728.0
78	4762.0
79	4796.0
80	3717.0
81	2638.0
82	2638.0
83	2676.0
84	2714.0
85	2714.0
86	1697.5
87	681.0
88	596.5
89	512.0
90	512.0
91	296.0
92	80.0
93	57.5
94	35.0
95	35.0
96	24.5
97	14.0
98	14.0
99	12.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009442785260013119
2	0.0
3	0.0
4	0.0
5	1.0045516234056512E-4
6	1.0045516234056512E-4
7	9.04096461065086E-4
8	0.001908648084470737
9	0.004620937467665995
10	2.0091032468113023E-4
11	0.004118661655963169
12	6.027309740433906E-4
13	1.0045516234056512E-4
14	1.0045516234056512E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	3.013654870216953E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0045516234056512E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	995469.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.978349503903914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.70397042586697	37.2810825093326
2	13.626237607397668	13.075287807019295
3	4.0465802753345965	5.82444728236821
4	1.6395586732319298	3.146532762259141
5	0.8107483868929021	1.9449184733036984
6	0.47478105867346426	1.3667526942521389
7	0.297164478604568	0.998022285024474
8	0.20684593867845266	0.7939301391502304
9	0.14631775570273425	0.6318075979559353
>10	0.7891659190747694	7.082975062656066
>50	0.10206455029778	3.4532465296167105
>100	0.1315229589051672	13.546955293542053
>500	0.018939306054760262	6.182232038039748
>1k	0.00610266528431164	4.671809525479737
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4865	0.48871436478684926	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3324	0.3339129596200384	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2023	0.2032207934149632	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1921	0.19297436685622554	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1808	0.1816229335117417	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1677	0.16846330724512767	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1624	0.16313918364107774	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1621	0.16283781815405604	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1614	0.16213463201767206	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1562	0.1569109635759627	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1494	0.15008001253680425	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1477	0.14837227477701467	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1461	0.14676499217956562	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1452	0.14586089571850053	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1434	0.14405270279637034	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1423	0.14294769601062413	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1422	0.14284724084828357	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1324	0.13300263493890818	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1322	0.13280172461422707	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1272	0.1277789664971988	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1272	0.1277789664971988	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1184	0.11893891221122908	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1160	0.11652798831505552	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1135	0.11401660925654139	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1099	0.11040022341228105	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1088	0.10929521662653484	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1088	0.10929521662653484	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1072	0.1076879340290858	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1054	0.10587974110695562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.004551623405651E-4
13	0.0	0.0	0.0	0.0	5.022758117028254E-4
14	0.0	0.0	0.0	0.0	5.022758117028254E-4
15	0.0	0.0	0.0	1.004551623405651E-4	6.027309740433906E-4
16	0.0	0.0	0.0	2.009103246811302E-4	6.027309740433906E-4
17	0.0	0.0	0.0	3.013654870216953E-4	6.027309740433906E-4
18	0.0	0.0	0.0	3.013654870216953E-4	6.027309740433906E-4
19	0.0	0.0	0.0	3.013654870216953E-4	6.027309740433906E-4
20	0.0	0.0	0.0	4.018206493622604E-4	6.027309740433906E-4
21	0.0	0.0	0.0	9.040964610650859E-4	6.027309740433906E-4
22	0.0	0.0	0.0	0.0013059171104273463	6.027309740433906E-4
23	0.0	0.0	0.0	0.0029131997078763877	6.027309740433906E-4
24	0.0	0.0	0.0	0.004620937467665995	6.027309740433906E-4
25	0.0	0.0	0.0	0.005625489091071646	6.027309740433906E-4
26	0.0	0.0	0.0	0.006730495876817862	6.027309740433906E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTAGA	35	0.002066498	22.856857	2
GTATAGA	40	0.0044820877	20.000753	1
ATGGGCG	115	4.5474735E-11	19.478994	5
CTAGCGG	365	0.0	18.84908	29
TCTAGCG	370	0.0	18.59436	28
ACCGTCC	70	1.786289E-5	18.286404	8
GTATTAG	170	0.0	17.883028	1
ACCGGTC	45	0.008847543	17.778448	8
GGTATAC	45	0.008847543	17.778448	1
CATTACG	45	0.008850103	17.777555	2
TGTAGGA	585	0.0	17.777554	2
ATTAGAG	280	0.0	17.714952	3
GTAGGAC	620	0.0	17.549047	3
ACACCGT	65	2.0994437E-4	17.231419	6
GGCCCGT	65	2.0994437E-4	17.231419	6
AATTAGA	255	0.0	16.940964	2
TAGGACG	540	0.0	16.593218	4
TAGCGGC	430	0.0	16.371887	30
AGCGGCG	425	0.0	16.188034	31
CTGTAGG	615	0.0	16.130688	1
>>END_MODULE