FastQCFastQC Report
Thu 2 Feb 2017
SRR4062946_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062946_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523152
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36510.23970030568190173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22330.1466038845761946No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20930.13741241845856486No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20460.13432671197621773No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19590.12861487231740498No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19590.12861487231740498No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18460.12119604609388951No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18440.12106473943506624No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17490.11482767314096032No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17400.11423679317625555No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17170.11272676659978781No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17020.11174196665861319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAA450.00885265717.77739313
ACCGTCG3850.017.0374768
TACCGTC3900.016.8184957
ATACCGT4100.016.777376
ATCGTTT4150.016.57741229
CATCGTT4150.016.57632328
GCATCGT4250.016.1862927
CGTCGTA4000.015.99965510
TAGGACG1102.0246989E-715.9970294
TAAACGC4050.015.80057328
CGCTTCG4200.015.61819732
GTAAACG4200.015.61717227
AACGCTT4100.015.60890730
TTTTCGG5350.015.55055629
CCGTCGT4250.015.4349619
TTCGGAA5450.015.26522431
GTCCTAT4950.015.212071
CGGCATC4650.015.1394625
TCGTTTA4650.015.13896330
CGTTTAT4650.015.13896331
Produced by FastQC (version 0.11.5)