##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062946_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1523152
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28020775339559	32.0	32.0	32.0	32.0	32.0
2	30.928733310923665	32.0	32.0	32.0	32.0	32.0
3	30.984565558788617	32.0	32.0	32.0	32.0	32.0
4	31.043192668886626	32.0	32.0	32.0	32.0	32.0
5	30.968081977373238	32.0	32.0	32.0	32.0	32.0
6	34.6357008361608	36.0	36.0	36.0	32.0	36.0
7	34.56810482473187	36.0	36.0	36.0	32.0	36.0
8	34.5601568326733	36.0	36.0	36.0	32.0	36.0
9	34.673798806685085	36.0	36.0	36.0	32.0	36.0
10	34.419594367469564	36.0	36.0	36.0	32.0	36.0
11	34.657153061546055	36.0	36.0	36.0	32.0	36.0
12	34.492191192999776	36.0	36.0	36.0	32.0	36.0
13	34.56431334495835	36.0	36.0	36.0	32.0	36.0
14	34.46946594955723	36.0	36.0	36.0	32.0	36.0
15	34.42507576394214	36.0	36.0	36.0	32.0	36.0
16	34.438778270323645	36.0	36.0	36.0	32.0	36.0
17	34.36623921972331	36.0	36.0	36.0	32.0	36.0
18	34.394095270859374	36.0	36.0	36.0	32.0	36.0
19	34.378285292603756	36.0	36.0	36.0	32.0	36.0
20	34.35558499742639	36.0	36.0	36.0	32.0	36.0
21	34.33961088584724	36.0	36.0	36.0	32.0	36.0
22	34.31662434215364	36.0	36.0	36.0	32.0	36.0
23	34.256548263075516	36.0	36.0	36.0	32.0	36.0
24	34.24389752303119	36.0	36.0	36.0	32.0	36.0
25	34.2218800224797	36.0	36.0	36.0	32.0	36.0
26	34.173523062701555	36.0	36.0	36.0	32.0	36.0
27	34.17132827189933	36.0	36.0	36.0	32.0	36.0
28	34.1643900280471	36.0	36.0	36.0	32.0	36.0
29	34.127347106526464	36.0	36.0	36.0	32.0	36.0
30	34.10613648539345	36.0	36.0	36.0	32.0	36.0
31	34.1236501675473	36.0	36.0	36.0	32.0	36.0
32	34.07329669002174	36.0	36.0	36.0	32.0	36.0
33	34.04513141170415	36.0	36.0	36.0	32.0	36.0
34	34.050785476433084	36.0	36.0	36.0	32.0	36.0
35	34.001138428732	36.0	36.0	36.0	32.0	36.0
36	33.97226737712323	36.0	36.0	36.0	32.0	36.0
37	33.97225424645735	36.0	36.0	36.0	32.0	36.0
38	33.377676686240115	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	2.0
9	5.0
10	8.0
11	21.0
12	9.0
13	7.0
14	206.0
15	414.0
16	532.0
17	638.0
18	806.0
19	976.0
20	1306.0
21	1850.0
22	2745.0
23	4171.0
24	6128.0
25	9226.0
26	13354.0
27	19291.0
28	27401.0
29	37210.0
30	50723.0
31	68131.0
32	93496.0
33	141451.0
34	323076.0
35	719965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65851407657769	17.000809146045274	11.293996083759433	26.046680693617606
2	16.88009427864226	19.73426981349225	37.08792508158729	26.297710826278202
3	19.067286339574274	23.574710726653596	28.347409048467647	29.01059388530448
4	12.747413349930964	15.278223685895101	35.87149704978436	36.10286591438958
5	14.914138575795455	36.218184396567125	33.31512547664317	15.552551550994254
6	34.33918808993736	35.21119732869207	16.886135236933484	13.563479344437084
7	30.54601940319771	30.43119165466851	20.622577800887765	18.400211141246007
8	28.425199176703465	32.22949883300671	19.62052201202118	19.724779978268646
9	27.72085987647958	14.337993196988608	18.419406111943086	39.521740814588725
10	16.06575889281353	26.161810470475466	31.261079086623685	26.51135155008732
11	37.65661889642603	21.141023317865123	21.966957384953556	19.23540040075529
12	25.232091223997248	23.618548389214684	28.21585862591491	22.933501760873163
13	29.577459239754944	19.149468458826078	24.998128607355557	26.274943694063417
14	24.010022232231616	19.649917860242912	24.84258188040294	31.497478027122533
15	25.439045228077617	26.58233916231556	22.39273492851622	25.5858806810906
16	26.12477388303967	25.585376183112878	23.384515379236305	24.905334554611144
17	24.32647357606792	25.60848360599221	24.967511424355628	25.097531393584237
18	25.390378530772985	24.397780014483875	26.011753868658953	24.200087586084187
19	25.755738332434913	24.887413192107662	25.422524715258294	23.934323760199128
20	25.912894237158156	23.92585229287202	24.969797802855815	25.19145566711401
21	27.215068116295054	24.06292082305521	24.224438087346698	24.497572973303033
22	26.001038710003595	24.1007640624723	24.765026332152146	25.133170895371965
23	24.422689279781483	23.857230090413065	25.430496188468886	26.289584441336565
24	24.841901917794907	24.997455591035013	24.991611658186397	25.16903083298368
25	25.124877125636203	24.196186610125306	25.063086998004465	25.615849266234026
26	24.79817606585495	25.225660631101178	25.354825322320735	24.621337980723137
27	25.626565552108467	24.630587153854993	24.62224848574383	25.120598808292705
28	24.649276755388964	24.51303849403867	25.49106506805019	25.346619682522174
29	24.58177553831749	24.86812091509705	25.619276867942318	24.93082667864314
30	24.63883060163823	24.827326755555088	25.776832634324027	24.757010008482656
31	25.195822625736835	24.842980784761224	24.356854809587528	25.604341779914407
32	24.867027650238565	24.68765203757433	24.539135333384547	25.906184978802553
33	24.439587164788062	24.362832224468477	25.186060147246046	26.011520463497412
34	25.341523551253974	24.48430846610701	25.274679816540868	24.899488166098145
35	26.075936400539497	24.301893879201998	25.204050676798172	24.418119043460333
36	24.739302385594115	25.11870187329318	24.7581476007152	25.383848140397507
37	25.873974811771138	24.874114666125635	24.67608615290874	24.575824369194486
38	24.697123692766688	24.618926059574648	25.281537742340436	25.402412505318228
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	447.0
1	349.5
2	252.0
3	252.0
4	608.5
5	965.0
6	965.0
7	1117.5
8	1270.0
9	1199.0
10	1128.0
11	1128.0
12	1456.5
13	1785.0
14	2388.5
15	2992.0
16	2992.0
17	4320.0
18	5648.0
19	5648.0
20	7034.5
21	8421.0
22	9011.0
23	9601.0
24	9601.0
25	11257.0
26	12913.0
27	12913.0
28	16806.5
29	20700.0
30	25878.0
31	31056.0
32	31056.0
33	40115.0
34	49174.0
35	49174.0
36	55579.0
37	61984.0
38	71776.5
39	81569.0
40	81569.0
41	88358.5
42	95148.0
43	107781.0
44	120414.0
45	120414.0
46	125735.0
47	131056.0
48	131056.0
49	137883.5
50	144711.0
51	146047.0
52	147383.0
53	147383.0
54	139609.5
55	131836.0
56	131836.0
57	127709.0
58	123582.0
59	110450.0
60	97318.0
61	97318.0
62	91773.0
63	86228.0
64	71331.5
65	56435.0
66	56435.0
67	47080.0
68	37725.0
69	37725.0
70	30363.0
71	23001.0
72	18168.5
73	13336.0
74	13336.0
75	10107.0
76	6878.0
77	6878.0
78	6735.5
79	6593.0
80	5115.0
81	3637.0
82	3637.0
83	3805.0
84	3973.0
85	3973.0
86	2649.5
87	1326.0
88	1141.5
89	957.0
90	957.0
91	682.5
92	408.0
93	362.5
94	317.0
95	317.0
96	282.0
97	247.0
98	247.0
99	492.5
100	738.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11784772629389582
2	0.0561992499763648
3	0.012999359223504942
4	0.0028230931647005683
5	0.0
6	1.969599882349234E-4
7	6.565332941164112E-5
8	4.595733058814879E-4
9	1.969599882349234E-4
10	7.878399529396935E-4
11	0.0018382932235259516
12	0.0028887464941122092
13	0.01457503912938433
14	0.009060159458806475
15	0.023569545258779162
16	0.010307572717627656
17	0.020286878788197106
18	0.0051866130235196485
19	0.006893599588222318
20	0.005514879670577855
21	0.006237066294105907
22	0.007156212905868883
23	0.009322772776453039
24	0.01345893252938643
25	0.016741598999968486
26	0.019039465529375927
27	0.008337972835278424
28	0.005580532999989496
29	0.010898452682332426
30	0.0028887464941122092
31	0.005908799647047701
32	0.005974452976459342
33	0.008075359517631858
34	0.012933705894093301
35	0.016150719035263716
36	0.014378079141149405
37	0.009125812788218116
38	0.0057774929882244185
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1523152.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.863841050905116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.87457778459138	47.37577555037563
2	11.679754767399778	13.51670946748744
3	3.0817758574112326	5.349701651032812
4	1.1588963869813715	2.6823278532303325
5	0.5713859707294378	1.6531293494502652
6	0.35192617337419907	1.221828009466676
7	0.23573744907297822	0.9548471998033257
8	0.16389752428190185	0.7586992234947865
9	0.11471744970390149	0.5974193047888582
>10	0.6174337775541073	6.417979873842264
>50	0.0634019942154031	2.602156345249242
>100	0.07200307909329245	9.20936751026451
>500	0.010041552220583786	3.9090973683662296
>1k	0.0044502333704859965	3.7509612931477117
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3651	0.23970030568190173	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2233	0.1466038845761946	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2093	0.13741241845856486	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2046	0.13432671197621773	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1959	0.12861487231740498	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1959	0.12861487231740498	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1846	0.12119604609388951	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1844	0.12106473943506624	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1749	0.11482767314096032	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1740	0.11423679317625555	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1717	0.11272676659978781	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1702	0.11174196665861319	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.565332941164113E-5	0.0
5	0.0	0.0	0.0	6.565332941164113E-5	0.0
6	0.0	0.0	0.0	6.565332941164113E-5	0.0
7	0.0	0.0	0.0	6.565332941164113E-5	0.0
8	0.0	0.0	0.0	6.565332941164113E-5	0.0
9	0.0	0.0	0.0	1.3130665882328226E-4	0.0
10	0.0	0.0	0.0	1.3130665882328226E-4	0.0
11	0.0	0.0	0.0	1.3130665882328226E-4	0.0
12	0.0	0.0	0.0	1.3130665882328226E-4	6.565332941164113E-5
13	0.0	0.0	0.0	1.3130665882328226E-4	6.565332941164113E-5
14	0.0	0.0	0.0	1.3130665882328226E-4	6.565332941164113E-5
15	0.0	0.0	0.0	1.9695998823492336E-4	1.3130665882328226E-4
16	0.0	0.0	0.0	2.626133176465645E-4	1.3130665882328226E-4
17	0.0	0.0	0.0	2.626133176465645E-4	1.3130665882328226E-4
18	0.0	0.0	0.0	2.626133176465645E-4	1.3130665882328226E-4
19	0.0	0.0	0.0	2.626133176465645E-4	1.3130665882328226E-4
20	0.0	0.0	0.0	3.939199764698467E-4	1.3130665882328226E-4
21	0.0	0.0	0.0	6.565332941164112E-4	1.3130665882328226E-4
22	0.0	0.0	0.0	0.001050453270586258	1.3130665882328226E-4
23	0.0	0.0	0.0	0.0017726398941143103	1.3130665882328226E-4
24	0.0	0.0	0.0	0.0022322131999957984	1.3130665882328226E-4
25	0.0	0.0	0.0	0.0029543998235238506	1.3130665882328226E-4
26	0.0	0.0	0.0	0.0035452797882286205	1.3130665882328226E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAA	45	0.008852657	17.777393	13
ACCGTCG	385	0.0	17.037476	8
TACCGTC	390	0.0	16.818495	7
ATACCGT	410	0.0	16.77737	6
ATCGTTT	415	0.0	16.577412	29
CATCGTT	415	0.0	16.576323	28
GCATCGT	425	0.0	16.18629	27
CGTCGTA	400	0.0	15.999655	10
TAGGACG	110	2.0246989E-7	15.997029	4
TAAACGC	405	0.0	15.800573	28
CGCTTCG	420	0.0	15.618197	32
GTAAACG	420	0.0	15.617172	27
AACGCTT	410	0.0	15.608907	30
TTTTCGG	535	0.0	15.550556	29
CCGTCGT	425	0.0	15.434961	9
TTCGGAA	545	0.0	15.265224	31
GTCCTAT	495	0.0	15.21207	1
CGGCATC	465	0.0	15.13946	25
TCGTTTA	465	0.0	15.138963	30
CGTTTAT	465	0.0	15.138963	31
>>END_MODULE