FastQCFastQC Report
Thu 2 Feb 2017
SRR4062946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523152
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42280.2775822767524187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27430.1800870825761316No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21350.1401698582938538No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21040.1381346050820929No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21000.13787199176444634No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19760.12973097891740287No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19580.12854921898799332No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18530.121655619399771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17650.11587812641154659No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17580.1154185531056651No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17420.11436809983507883No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17340.1138428731997857No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16160.10609578032921205No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15740.10333834049392314No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15560.10215658056451357No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15510.1018283139174554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATA308.418932E-426.6662332
TTACACG350.00206625522.8582694
ATACCGT4250.015.8125436
TACCGTC4250.015.8125437
TGCACCG951.43233265E-515.1586425
ATCGCTC1807.2759576E-1215.11086516
TAGCGGC5300.015.09409230
GTATTAC1501.9699655E-914.9345591
GGACCGT756.2436715E-414.934076
ACCGTCG4500.014.9335788
AGGCGTA756.246782E-414.93308812
CATCGTT3750.014.93308828
TCTAGCG5400.014.81457128
CTAGCGG5400.014.81457129
GTTTACG650.004161951614.7694739
AAGACGG4350.014.7133675
ATCGTTT3700.014.70246129
CCGTCGT4600.014.6089359
CAAGACG4500.014.5784954
CGTCGTA4650.014.45137610
Produced by FastQC (version 0.11.5)