FastQCFastQC Report
Thu 2 Feb 2017
SRR4062945_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062945_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247772
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT52822.1317985890253945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33071.334694800058118No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21800.8798411442778037No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA20740.8370598776294335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19870.801946951229356No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC17030.6873254443601375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16480.6651276173256058No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11270.45485365578031417No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA10530.42498748849748963No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG8700.35112926400077493No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA8620.3479004891593885No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA6860.2768674426488869No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6060.24457969423502252No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5610.22641783575222382No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5110.2062379929935586No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4860.19614807161422598No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA4660.18807613451075988No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4470.1804077942624671No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA4420.1783898099866006No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.17556463200038747No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA4140.16708909804174807No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC4030.16264953263484172No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA4030.16264953263484172No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3590.1448912710072163No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT3510.14166249616582988No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC3200.12915099365545743No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3190.12874739680028413No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3180.12834379994511083No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3070.12390423453820448No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA3050.12309704082785787No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA3020.12188625026233796No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2990.12067545969681803No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC2980.12027186284164473No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.11986826598647142No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT2880.11623589428991168No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG2830.11421791001404516No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2600.10493518234505916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGA252.894431E-431.98894
TGTCCAC308.3953585E-426.6681810
AATGTAA401.5902001E-423.9965232
GTACTTA350.00205647722.8676741
GACAGTG350.002061313522.8584429
CCAATAA350.002061313522.85844222
CTTACTT350.002061313522.85844220
ATTATTC350.002063735222.8538273
TTTGCCT350.002066159222.8492164
GGCCTAA350.002066159222.8492166
TGTAGGC502.7762791E-522.410322
AAGTACT453.5342382E-421.3259334
AGGACCT604.710064E-621.3259325
TCAATGT707.7445475E-720.56844330
CATGTAA555.8161546E-520.36068332
AGTACTC555.8252022E-520.3565735
TTGTAGG400.0044618120.0092161
AAACATG400.004472255720.00113726
GAAAGCG400.00447748619.99709930
AAGTTCC400.00447748619.99709931
Produced by FastQC (version 0.11.5)