##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062945_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247772
Sequences flagged as poor quality	0
Sequence length	38
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.758184944222915	32.0	32.0	32.0	32.0	32.0
2	30.537925996480634	32.0	32.0	32.0	32.0	32.0
3	30.69413008733836	32.0	32.0	32.0	32.0	32.0
4	30.644867055195906	32.0	32.0	32.0	32.0	32.0
5	30.73702839707473	32.0	32.0	32.0	32.0	32.0
6	34.27621361574351	36.0	36.0	36.0	32.0	36.0
7	34.01886815297935	36.0	36.0	36.0	32.0	36.0
8	33.96422517475744	36.0	36.0	36.0	32.0	36.0
9	33.96380139805951	36.0	36.0	36.0	32.0	36.0
10	33.8732019760102	36.0	36.0	36.0	32.0	36.0
11	34.212752046236055	36.0	36.0	36.0	32.0	36.0
12	34.04660332886686	36.0	36.0	36.0	32.0	36.0
13	34.106783655941754	36.0	36.0	36.0	32.0	36.0
14	34.03979061395154	36.0	36.0	36.0	32.0	36.0
15	34.06047898874772	36.0	36.0	36.0	32.0	36.0
16	34.01138950325299	36.0	36.0	36.0	32.0	36.0
17	33.92949566536978	36.0	36.0	36.0	32.0	36.0
18	33.98781137497377	36.0	36.0	36.0	32.0	36.0
19	33.914639265130845	36.0	36.0	36.0	32.0	36.0
20	33.744987327058745	36.0	36.0	36.0	32.0	36.0
21	33.674862373472386	36.0	36.0	36.0	27.0	36.0
22	33.70101544968762	36.0	36.0	36.0	27.0	36.0
23	33.729561855254026	36.0	36.0	36.0	27.0	36.0
24	33.745693621555304	36.0	36.0	36.0	27.0	36.0
25	33.697076344381124	36.0	36.0	36.0	27.0	36.0
26	33.670600390681756	36.0	36.0	36.0	27.0	36.0
27	33.69276996593643	36.0	36.0	36.0	27.0	36.0
28	33.65218830214875	36.0	36.0	36.0	27.0	36.0
29	33.598530100253456	36.0	36.0	36.0	27.0	36.0
30	33.50961367709023	36.0	36.0	36.0	21.0	36.0
31	33.501279402030896	36.0	36.0	36.0	21.0	36.0
32	33.44070355003794	36.0	36.0	36.0	21.0	36.0
33	33.291275850378575	36.0	36.0	36.0	21.0	36.0
34	33.20335227547907	36.0	36.0	36.0	21.0	36.0
35	33.067856739260286	36.0	36.0	36.0	14.0	36.0
36	33.04313239591237	36.0	36.0	36.0	14.0	36.0
37	32.98939347464604	36.0	36.0	36.0	14.0	36.0
38	32.235272750754724	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	1.0
14	103.0
15	190.0
16	225.0
17	289.0
18	373.0
19	477.0
20	600.0
21	754.0
22	978.0
23	1318.0
24	1757.0
25	2334.0
26	3203.0
27	4450.0
28	6070.0
29	8108.0
30	10688.0
31	13950.0
32	17984.0
33	25052.0
34	50998.0
35	97867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.0869342083463	17.539995878171684	12.54652210633509	20.826547807146927
2	15.011670449130571	21.08313074941244	39.87336149317137	24.031837308285613
3	19.72527428170083	30.01800289015008	27.48347044054606	22.773252387603033
4	11.2421044134563	16.581841664480454	35.61277823744274	36.56327568462051
5	11.693411684936152	40.52112425939977	31.518896404759218	16.266567650904864
6	28.786545695235944	36.873415882343444	19.367805886056537	14.972232536364077
7	27.716610432171514	34.211694622475505	19.55991798911903	18.51177695623396
8	30.88524934213713	29.38951939686486	18.74707392279999	20.97815733819802
9	30.536138062412217	14.117010800251844	19.309284342056408	36.03756679527953
10	17.966921201749997	26.63174208546567	29.17642025733336	26.22491645545098
11	34.41443913936659	24.54564900654233	21.24259901279811	19.797312841292968
12	26.167518939011742	26.559012628698504	27.57084219575492	19.702626236534837
13	30.695395246706276	21.863698010849912	26.371173469387756	21.06973327305606
14	25.525912173070715	20.906926057474976	26.861882466903452	26.705279302550856
15	25.55902288572478	25.770965124400004	28.722412185265778	19.947599804609442
16	23.05633814186653	27.60883779878428	26.838315425785254	22.496508633563938
17	20.54714338192255	26.207901567476576	27.76649725701691	25.478457793583964
18	21.94095211188021	25.060036728351463	31.786975561519988	21.21203559824834
19	22.90684533419438	26.445350339037777	30.505731352922183	20.142072973845657
20	24.318902818026977	22.862262978180674	32.430719803682564	20.38811440010978
21	26.40991592772125	23.1673010094324	27.823767067721977	22.599015995124372
22	24.332516679515173	25.107059682998393	28.370715090067367	22.189708547419066
23	22.639118168205137	25.992000226027923	29.258507327744525	22.11037427802242
24	21.78284561049445	27.835721493440968	29.283551967709386	21.0978809283552
25	23.488594575611646	25.775330698776504	29.33723000165501	21.39884472395684
26	20.78659169166058	26.123111328195957	31.338529913977887	21.751767066165577
27	22.282363008169458	27.708757144241012	29.828215312086282	20.180664535503247
28	21.100321273469916	26.695565134563537	30.149254936148917	22.054858655817632
29	20.28149571338618	27.798408059802703	30.87492129098923	21.045174935821883
30	21.84122371554264	27.195382814707187	30.579973362392543	20.38342010735763
31	20.884962181448323	28.12739645304768	28.90556259636264	22.082078769141354
32	19.644983310663818	29.361123331570898	29.1242033717706	21.869689985994683
33	20.48321150795733	28.397353880181953	28.934569480826127	22.184865131034595
34	20.303052319714546	28.956673367077574	30.066681197678268	20.673593115529616
35	21.485601724402393	28.700885613026667	29.06457628623788	20.74893637633306
36	20.92401834151382	29.884477525187293	28.325610307414106	20.865893825884786
37	21.875908166230747	30.350108172688817	27.32530918014789	20.448674480932546
38	20.59072819883598	31.44751818277218	27.1962609278259	20.765492690565946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	148.0
1	177.0
2	206.0
3	206.0
4	511.5
5	817.0
6	817.0
7	1132.0
8	1447.0
9	1516.5
10	1586.0
11	1586.0
12	1984.5
13	2383.0
14	2850.5
15	3318.0
16	3318.0
17	4570.0
18	5822.0
19	5822.0
20	6534.0
21	7246.0
22	5865.0
23	4484.0
24	4484.0
25	4180.0
26	3876.0
27	3876.0
28	5357.0
29	6838.0
30	8585.0
31	10332.0
32	10332.0
33	10109.5
34	9887.0
35	9887.0
36	10240.5
37	10594.0
38	11955.0
39	13316.0
40	13316.0
41	13980.0
42	14644.0
43	17032.5
44	19421.0
45	19421.0
46	23233.0
47	27045.0
48	27045.0
49	24741.5
50	22438.0
51	18500.5
52	14563.0
53	14563.0
54	13342.0
55	12121.0
56	12121.0
57	11990.5
58	11860.0
59	10909.5
60	9959.0
61	9959.0
62	9496.5
63	9034.0
64	8419.5
65	7805.0
66	7805.0
67	6901.5
68	5998.0
69	5998.0
70	5198.5
71	4399.0
72	3501.0
73	2603.0
74	2603.0
75	2098.5
76	1594.0
77	1594.0
78	1253.5
79	913.0
80	704.0
81	495.0
82	495.0
83	365.0
84	235.0
85	235.0
86	164.5
87	94.0
88	68.5
89	43.0
90	43.0
91	32.0
92	21.0
93	19.0
94	17.0
95	17.0
96	18.5
97	20.0
98	20.0
99	85.0
100	150.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12471142824855108
2	0.05569636601391602
3	0.013722293075892351
4	0.0028251779862131315
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	4.035968551733045E-4
12	0.0012107905655199135
13	0.011300711944852526
14	0.004843162262079654
15	0.025426601875918182
16	0.00726474339311948
17	0.019776245903491918
18	0.0028251779862131315
19	0.004843162262079654
20	0.004035968551733045
21	0.00363237169655974
22	0.005246759117252958
23	0.0060539528275995675
24	0.008879130813812698
25	0.015740277351758876
26	0.018161858482798703
27	0.00807193710346609
28	0.003228774841386436
29	0.009686324524159308
30	8.07193710346609E-4
31	0.004035968551733045
32	0.00363237169655974
33	0.005246759117252958
34	0.010493518234505917
35	0.013722293075892351
36	0.011300711944852526
37	0.00807193710346609
38	0.004035968551733045
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	247772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.544306862760926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19639379280015	27.82759956734417
2	13.538983476785827	10.437014674781654
3	5.301459655295178	6.130232633227322
4	2.6062281418190194	4.0182102901054195
5	1.4711733785679881	2.835267907592464
6	0.9528596259764193	2.2036388292462425
7	0.6387300789512261	1.7233585715900102
8	0.42093359301375893	1.2979674862373474
9	0.3518250926682164	1.2204768900440728
>10	2.1297983288308098	16.182215908173646
>50	0.24083265271931478	6.494680593448816
>100	0.13507570522083306	9.75856836123533
>500	0.006282590940503864	1.6531327187898552
>1k	0.008376787920671818	6.085836979158259
>5k	0.0010470984900839773	2.1317985890253945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5282	2.1317985890253945	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3307	1.334694800058118	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2180	0.8798411442778037	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2074	0.8370598776294335	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1987	0.801946951229356	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1703	0.6873254443601375	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1648	0.6651276173256058	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1127	0.45485365578031417	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1053	0.42498748849748963	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	870	0.35112926400077493	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	862	0.3479004891593885	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	686	0.2768674426488869	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	606	0.24457969423502252	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	561	0.22641783575222382	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	511	0.2062379929935586	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	486	0.19614807161422598	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	466	0.18807613451075988	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	447	0.1804077942624671	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	442	0.1783898099866006	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	435	0.17556463200038747	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	414	0.16708909804174807	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	403	0.16264953263484172	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	403	0.16264953263484172	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	359	0.1448912710072163	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	351	0.14166249616582988	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	320	0.12915099365545743	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	319	0.12874739680028413	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	318	0.12834379994511083	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	307	0.12390423453820448	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	305	0.12309704082785787	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	302	0.12188625026233796	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	299	0.12067545969681803	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	298	0.12027186284164473	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	297	0.11986826598647142	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	288	0.11623589428991168	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	283	0.11421791001404516	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	260	0.10493518234505916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.035968551733045E-4	0.0
10	0.0	0.0	0.0	4.035968551733045E-4	0.0
11	0.0	0.0	0.0	4.035968551733045E-4	0.0
12	0.0	0.0	0.0	4.035968551733045E-4	0.0
13	0.0	0.0	0.0	4.035968551733045E-4	0.0
14	0.0	0.0	0.0	8.07193710346609E-4	0.0
15	0.0	0.0	0.0	8.07193710346609E-4	0.0
16	0.0	0.0	0.0	8.07193710346609E-4	0.0
17	0.0	0.0	0.0	0.0012107905655199135	0.0
18	0.0	0.0	0.0	0.0012107905655199135	0.0
19	0.0	0.0	0.0	0.0012107905655199135	0.0
20	0.0	0.0	0.0	0.001614387420693218	0.0
21	0.0	0.0	0.0	0.002421581131039827	0.0
22	0.0	0.0	0.0	0.004035968551733045	0.0
23	0.0	0.0	0.0	0.010089921379332612	0.0
24	0.0	0.0	0.0	0.016547471062105485	0.0
25	0.0	0.0	0.0	0.01977624590349192	0.0
26	0.0	0.0	0.0	0.025830198731091488	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGA	25	2.894431E-4	31.9889	4
TGTCCAC	30	8.3953585E-4	26.66818	10
AATGTAA	40	1.5902001E-4	23.99652	32
GTACTTA	35	0.002056477	22.867674	1
GACAGTG	35	0.0020613135	22.858442	9
CCAATAA	35	0.0020613135	22.858442	22
CTTACTT	35	0.0020613135	22.858442	20
ATTATTC	35	0.0020637352	22.853827	3
TTTGCCT	35	0.0020661592	22.849216	4
GGCCTAA	35	0.0020661592	22.849216	6
TGTAGGC	50	2.7762791E-5	22.41032	2
AAGTACT	45	3.5342382E-4	21.325933	4
AGGACCT	60	4.710064E-6	21.325932	5
TCAATGT	70	7.7445475E-7	20.568443	30
CATGTAA	55	5.8161546E-5	20.360683	32
AGTACTC	55	5.8252022E-5	20.356573	5
TTGTAGG	40	0.00446181	20.009216	1
AAACATG	40	0.0044722557	20.001137	26
GAAAGCG	40	0.004477486	19.997099	30
AAGTTCC	40	0.004477486	19.997099	31
>>END_MODULE