FastQCFastQC Report
Thu 2 Feb 2017
SRR4062945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247772
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT64532.604410506433334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT43071.7382916552314225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT29481.1898035290509015No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC21640.873383594595031No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21450.8657152543467381No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.79387501412589No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA16060.648176549408327No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA9570.3862421904008524No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9360.377766656442213No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA8220.3317566149524563No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7910.31924511244208387No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA7350.29664368855237877No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7230.2918005262902991No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.2809034112006199No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA6790.2740422646626738No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.2526516313384886No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA5940.23973653197294284No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC5850.23610416027638315No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5490.22157467349014415No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG4950.1997804433107857No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA4920.19856965274526583No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.18202218168316034No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC4190.16910708231761457No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA4150.16749269489692137No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT4150.16749269489692137No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3910.15780637037276207No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3740.15094522383481587No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA3680.14852364270377605No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3620.14610206157273622No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT3470.14004810874513665No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA3460.13964451188996335No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3180.12834379994511083No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA3090.12471142824855108No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3040.12269344397268457No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3020.12188625026233796No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA2900.11704308800025831No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC2830.11421791001404516No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC2680.10816395718644561No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA2620.10574237605540578No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2590.10453158548988585No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA2590.10453158548988585No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC2520.10170640750367273No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC2480.1000920200829795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGTG200.003745103532.0048455
ACACCTA200.003745103532.0048456
ACCTACT200.003745103532.0048458
TAAGGCG302.2369097E-532.0048455
TGCAAAC200.003748807831.99838312
CTAAGGC356.4128355E-527.4271874
CCGTTAT308.3907234E-426.6707049
TACACCT308.3907234E-426.6707045
ATCGGTC308.4006245E-426.66532116
AGGCGCT451.2315286E-524.8926587
ACAGATC401.5870063E-424.0036338
GAGAGAA401.5891815E-423.99878715
CCTTAGG350.002060181722.8606031
GTCCTAA350.002060181722.8606031
TTAGGAC703.0611773E-822.855993
ACCGCTC350.002062601722.8559923
GACCGCT350.002062601722.8559922
ACCCGCA350.002062601722.8559924
TTGTGCA453.522358E-421.3365659
GGACCGT453.522358E-421.3365656
Produced by FastQC (version 0.11.5)