##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247772
Sequences flagged as poor quality	0
Sequence length	38
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.56914421322829	32.0	32.0	32.0	32.0	32.0
2	31.236063800590866	32.0	32.0	32.0	32.0	32.0
3	31.34702064801511	32.0	32.0	32.0	32.0	32.0
4	31.48707682869735	32.0	32.0	32.0	32.0	32.0
5	31.318748688310222	32.0	32.0	32.0	32.0	32.0
6	34.813663367935035	36.0	36.0	36.0	32.0	36.0
7	34.86090437983307	36.0	36.0	36.0	32.0	36.0
8	34.846334533361315	36.0	36.0	36.0	32.0	36.0
9	34.90644221300228	36.0	36.0	36.0	32.0	36.0
10	34.78414025797911	36.0	36.0	36.0	32.0	36.0
11	34.95908335082253	36.0	36.0	36.0	36.0	36.0
12	34.859612869896516	36.0	36.0	36.0	32.0	36.0
13	34.89051224512858	36.0	36.0	36.0	32.0	36.0
14	34.82002809034112	36.0	36.0	36.0	32.0	36.0
15	34.79318889947209	36.0	36.0	36.0	32.0	36.0
16	34.78321602118076	36.0	36.0	36.0	32.0	36.0
17	34.76064688503947	36.0	36.0	36.0	32.0	36.0
18	34.71419692297758	36.0	36.0	36.0	32.0	36.0
19	34.67118157015321	36.0	36.0	36.0	32.0	36.0
20	34.60171447944077	36.0	36.0	36.0	32.0	36.0
21	34.56304586474662	36.0	36.0	36.0	32.0	36.0
22	34.57351920313837	36.0	36.0	36.0	32.0	36.0
23	34.51939686485963	36.0	36.0	36.0	32.0	36.0
24	34.52106371987149	36.0	36.0	36.0	32.0	36.0
25	34.47836317259416	36.0	36.0	36.0	32.0	36.0
26	34.4102198795667	36.0	36.0	36.0	32.0	36.0
27	34.36370937797653	36.0	36.0	36.0	32.0	36.0
28	34.32171108922719	36.0	36.0	36.0	32.0	36.0
29	34.259032497618776	36.0	36.0	36.0	32.0	36.0
30	34.181513649645645	36.0	36.0	36.0	32.0	36.0
31	34.1595337649129	36.0	36.0	36.0	32.0	36.0
32	34.07529502930113	36.0	36.0	36.0	32.0	36.0
33	33.94425116639491	36.0	36.0	36.0	32.0	36.0
34	33.85493921831361	36.0	36.0	36.0	32.0	36.0
35	33.72981612127278	36.0	36.0	36.0	32.0	36.0
36	33.65515070306572	36.0	36.0	36.0	27.0	36.0
37	33.5924640395202	36.0	36.0	36.0	27.0	36.0
38	33.19846875353147	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	7.0
22	23.0
23	81.0
24	200.0
25	538.0
26	1150.0
27	2258.0
28	3983.0
29	6255.0
30	9253.0
31	12776.0
32	17400.0
33	25794.0
34	56001.0
35	112050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.456912209889	18.866195761856712	13.357416750756812	21.319475277497478
2	14.65016224593578	22.0715012188625	38.48699610932631	24.7913404258754
3	19.216053468511372	29.647417787320602	28.32684887719355	22.809679866974477
4	10.897966267238699	17.419310573069488	35.54411129631797	36.138611863373846
5	11.46013270264598	40.39156966888914	31.530197116704066	16.61810051176081
6	29.117730152964445	37.0755135811438	18.25321871090124	15.553537554990516
7	26.99702947919021	34.336556778922215	19.906525459300635	18.759888282586935
8	30.559793356742137	29.777212737619568	18.651572022440167	21.01142188319813
9	29.45533065606522	13.856678707646358	19.593970092628098	37.09402054366032
10	17.740906962852947	26.597032755920765	29.45288410312707	26.20917617809922
11	34.15172625342062	24.226879020995955	21.294629523494322	20.3267652020891
12	25.483113234961742	26.978463724129025	28.097252268250944	19.441170772658293
13	30.932873771047575	21.58637779894419	26.505416269796427	20.975332160211806
14	25.319245112442086	20.799363931356247	27.165297128004777	26.71609382819689
15	25.74786497263613	25.498845712994207	28.782509726684207	19.970779587685453
16	22.946499200878225	26.904573559562824	27.375167492694896	22.77375974686405
17	20.486576368596936	25.600552120497877	28.69250762798056	25.220363882924623
18	21.9173272201863	23.643510969762524	32.83623654004488	21.602925270006295
19	22.849635955636632	25.735757066980934	31.80262499394605	19.611981983436387
20	23.86791082123888	22.190158694283454	33.87509484526097	20.0668356392167
21	25.678146352882298	22.586581964798143	29.391656004940046	22.343615677379518
22	23.602344090534846	24.145585457598116	30.281872043653035	21.970198408214003
23	22.386891068329497	24.550187674052548	30.902449852685958	22.160471404931993
24	21.23928450349515	26.908609528114557	30.639055260481413	21.213050707908884
25	23.153947984437306	24.440211161874625	30.752869573640286	21.652971280047787
26	20.57133170818333	24.71990378250973	32.92341346076231	21.78535104854463
27	22.199934617045578	26.170940101948975	31.602164902268626	20.026960378736817
28	21.028606945094683	24.799412362978867	32.0270248454224	22.144955846504043
29	20.41836850007265	25.7922606267052	32.66309348917553	21.126277384046624
30	21.738533813344528	25.260723568441957	32.47421016095443	20.52653245725909
31	20.74972151816993	25.99446265114702	30.761748704454096	22.494067126228952
32	19.69350854818139	27.16166475630822	31.13547939234457	22.009347303165814
33	20.484154787465897	26.62205576094151	30.686276092536684	22.207513359055906
34	20.123742795796133	27.076505819866654	32.09523271394669	20.70451867039052
35	22.0186300308348	26.856949130652374	30.512325847956994	20.612094990555835
36	20.83165167977011	27.641541417109277	30.377120901473937	21.149686001646675
37	22.20065221251796	28.073390052144713	29.056551991346886	20.669405743990442
38	20.71549939258428	28.789487066686576	29.153936497814513	21.341077042914627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	75.5
2	96.0
3	96.0
4	417.5
5	739.0
6	739.0
7	1100.0
8	1461.0
9	1625.0
10	1789.0
11	1789.0
12	2215.5
13	2642.0
14	3156.5
15	3671.0
16	3671.0
17	5339.0
18	7007.0
19	7007.0
20	7535.5
21	8064.0
22	6591.0
23	5118.0
24	5118.0
25	4271.0
26	3424.0
27	3424.0
28	4883.5
29	6343.0
30	7904.0
31	9465.0
32	9465.0
33	9309.5
34	9154.0
35	9154.0
36	9623.5
37	10093.0
38	11419.0
39	12745.0
40	12745.0
41	13381.0
42	14017.0
43	16188.5
44	18360.0
45	18360.0
46	23104.0
47	27848.0
48	27848.0
49	25707.0
50	23566.0
51	19325.5
52	15085.0
53	15085.0
54	13790.0
55	12495.0
56	12495.0
57	11971.5
58	11448.0
59	10590.0
60	9732.0
61	9732.0
62	9250.5
63	8769.0
64	8313.0
65	7857.0
66	7857.0
67	7021.0
68	6185.0
69	6185.0
70	5351.0
71	4517.0
72	3602.5
73	2688.0
74	2688.0
75	2126.5
76	1565.0
77	1565.0
78	1246.5
79	928.0
80	717.0
81	506.0
82	506.0
83	351.0
84	196.0
85	196.0
86	144.0
87	92.0
88	58.0
89	24.0
90	24.0
91	19.5
92	15.0
93	10.5
94	6.0
95	6.0
96	5.0
97	4.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008879130813812698
2	0.0
3	0.0
4	4.035968551733045E-4
5	0.0
6	8.07193710346609E-4
7	0.001614387420693218
8	8.07193710346609E-4
9	0.0028251779862131315
10	0.0
11	0.004035968551733045
12	0.001614387420693218
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.035968551733045E-4
22	0.0
23	8.07193710346609E-4
24	0.0
25	0.0
26	0.0
27	4.035968551733045E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.035968551733045E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	247772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.2428845874433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.50711160990357	24.496311124743716
2	14.732827924234442	10.384547083609124
3	5.7854836123771785	6.116913937006603
4	2.8240305993907606	3.981079379429475
5	1.7212157302856097	3.0330303666273832
6	0.9940221250085889	2.10193242174257
7	0.7214676713772016	1.7798621313142728
8	0.5313666659032088	1.4981515264033063
9	0.3802020109479856	1.205947403257834
>10	2.346487712145851	16.236297886766867
>50	0.2725544536313873	6.5297935198488934
>100	0.1614713359748975	10.414009654036775
>500	0.013742241359565746	3.508467462021536
>1k	0.006871120679782873	6.10924559675831
>5k	0.0011451867799638121	2.604410506433334
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6453	2.604410506433334	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4307	1.7382916552314225	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2948	1.1898035290509015	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2164	0.873383594595031	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2145	0.8657152543467381	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1967	0.79387501412589	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1606	0.648176549408327	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	957	0.3862421904008524	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	936	0.377766656442213	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	822	0.3317566149524563	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	791	0.31924511244208387	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	735	0.29664368855237877	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	723	0.2918005262902991	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	696	0.2809034112006199	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	679	0.2740422646626738	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	626	0.2526516313384886	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	594	0.23973653197294284	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	585	0.23610416027638315	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	549	0.22157467349014415	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	495	0.1997804433107857	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	492	0.19856965274526583	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.18202218168316034	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	419	0.16910708231761457	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	415	0.16749269489692137	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	415	0.16749269489692137	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	391	0.15780637037276207	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	374	0.15094522383481587	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	368	0.14852364270377605	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	362	0.14610206157273622	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	347	0.14004810874513665	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	346	0.13964451188996335	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	318	0.12834379994511083	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	309	0.12471142824855108	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	304	0.12269344397268457	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	302	0.12188625026233796	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	290	0.11704308800025831	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	283	0.11421791001404516	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	268	0.10816395718644561	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	262	0.10574237605540578	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	259	0.10453158548988585	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	259	0.10453158548988585	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	252	0.10170640750367273	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	248	0.1000920200829795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	4.035968551733045E-4	0.0
13	0.0	0.0	0.0	4.035968551733045E-4	0.0
14	0.0	0.0	0.0	8.07193710346609E-4	0.0
15	0.0	0.0	0.0	8.07193710346609E-4	0.0
16	0.0	0.0	0.0	8.07193710346609E-4	0.0
17	0.0	0.0	0.0	0.0012107905655199135	0.0
18	0.0	0.0	0.0	0.0012107905655199135	0.0
19	0.0	0.0	0.0	0.0012107905655199135	0.0
20	0.0	0.0	4.035968551733045E-4	0.001614387420693218	0.0
21	0.0	0.0	4.035968551733045E-4	0.0020179842758665223	0.0
22	0.0	0.0	4.035968551733045E-4	0.0036323716965597406	0.0
23	0.0	0.0	4.035968551733045E-4	0.009686324524159308	0.0
24	0.0	0.0	4.035968551733045E-4	0.016951067917278788	0.0
25	0.0	0.0	4.035968551733045E-4	0.020179842758665224	0.0
26	0.0	0.0	4.035968551733045E-4	0.025830198731091488	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTGTG	20	0.0037451035	32.004845	5
ACACCTA	20	0.0037451035	32.004845	6
ACCTACT	20	0.0037451035	32.004845	8
TAAGGCG	30	2.2369097E-5	32.004845	5
TGCAAAC	20	0.0037488078	31.998383	12
CTAAGGC	35	6.4128355E-5	27.427187	4
CCGTTAT	30	8.3907234E-4	26.670704	9
TACACCT	30	8.3907234E-4	26.670704	5
ATCGGTC	30	8.4006245E-4	26.665321	16
AGGCGCT	45	1.2315286E-5	24.892658	7
ACAGATC	40	1.5870063E-4	24.003633	8
GAGAGAA	40	1.5891815E-4	23.998787	15
CCTTAGG	35	0.0020601817	22.860603	1
GTCCTAA	35	0.0020601817	22.860603	1
TTAGGAC	70	3.0611773E-8	22.85599	3
ACCGCTC	35	0.0020626017	22.85599	23
GACCGCT	35	0.0020626017	22.85599	22
ACCCGCA	35	0.0020626017	22.85599	24
TTGTGCA	45	3.522358E-4	21.336565	9
GGACCGT	45	3.522358E-4	21.336565	6
>>END_MODULE