FastQCFastQC Report
Thu 2 Feb 2017
SRR4062944_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062944_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587278
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53940.3398270498299605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33090.2084700978656543No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT28980.18257671308995652No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27110.170795538021695No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21270.13400299128445048No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20990.13223896507102095No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20830.13123095009191837No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20440.1287739135803558No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20170.1270728883031202No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19220.12108779936469856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18830.118630762853136No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18400.1159217225967978No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18390.11585872166060389No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17970.11321268234045959No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17250.10867661493449791No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16140.1016835110169737No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16090.10136850633600414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5800.020.69006229
TCTAGCG5900.020.60927628
TAGCGGC6000.019.733130
CTCGACG953.7913196E-818.5266813
CGGTCCA7050.017.70247710
CATCGTT4550.017.23002228
CCGGTCC7900.016.8104579
AGCGGCG7250.016.55152731
ATCGTTT4950.016.48517429
GCCGGTC7600.016.4198258
CGCCGGT7450.016.3209277
GCGGCGC7300.016.21898732
TCTTGCG7700.016.2121982
GTGTACG600.002431369916.0109081
TCGACGC1102.0201333E-716.00031514
GTGTAGG2350.015.6702521
TCCTACA1950.015.5939832
CTTGCGC8350.015.5197963
AACCGCG2900.015.447127
ACCTCTA8250.015.32103125
Produced by FastQC (version 0.11.5)