##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062944_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587278
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206755212382458	32.0	32.0	32.0	32.0	32.0
2	30.836033133452364	32.0	32.0	32.0	32.0	32.0
3	30.900268887995676	32.0	32.0	32.0	32.0	32.0
4	30.949469469116313	32.0	32.0	32.0	32.0	32.0
5	30.87717715485252	32.0	32.0	32.0	32.0	32.0
6	34.515800634797436	36.0	36.0	36.0	32.0	36.0
7	34.436433315399064	36.0	36.0	36.0	32.0	36.0
8	34.41678206338146	36.0	36.0	36.0	32.0	36.0
9	34.53815966705265	36.0	36.0	36.0	32.0	36.0
10	34.28254848867054	36.0	36.0	36.0	32.0	36.0
11	34.53546826705845	36.0	36.0	36.0	32.0	36.0
12	34.35708174623475	36.0	36.0	36.0	32.0	36.0
13	34.44865234697387	36.0	36.0	36.0	32.0	36.0
14	34.340816164528206	36.0	36.0	36.0	32.0	36.0
15	34.29204524979241	36.0	36.0	36.0	32.0	36.0
16	34.3099091652502	36.0	36.0	36.0	32.0	36.0
17	34.227552451429425	36.0	36.0	36.0	32.0	36.0
18	34.25749490637431	36.0	36.0	36.0	32.0	36.0
19	34.248209198388686	36.0	36.0	36.0	32.0	36.0
20	34.21926719831057	36.0	36.0	36.0	32.0	36.0
21	34.20342435288588	36.0	36.0	36.0	32.0	36.0
22	34.18404400489391	36.0	36.0	36.0	32.0	36.0
23	34.13003897237913	36.0	36.0	36.0	32.0	36.0
24	34.12797758174686	36.0	36.0	36.0	32.0	36.0
25	34.10317474317668	36.0	36.0	36.0	32.0	36.0
26	34.056558460458724	36.0	36.0	36.0	32.0	36.0
27	34.0620080414395	36.0	36.0	36.0	32.0	36.0
28	34.0530562384157	36.0	36.0	36.0	32.0	36.0
29	34.01424325165472	36.0	36.0	36.0	32.0	36.0
30	33.997692275707216	36.0	36.0	36.0	32.0	36.0
31	34.00692632292516	36.0	36.0	36.0	32.0	36.0
32	33.96180190237627	36.0	36.0	36.0	32.0	36.0
33	33.920428557568364	36.0	36.0	36.0	32.0	36.0
34	33.923027976195726	36.0	36.0	36.0	32.0	36.0
35	33.87769439253867	36.0	36.0	36.0	32.0	36.0
36	33.84084073489332	36.0	36.0	36.0	32.0	36.0
37	33.84181535937624	36.0	36.0	36.0	32.0	36.0
38	33.23415368952383	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	3.0
9	6.0
10	22.0
11	17.0
12	6.0
13	12.0
14	176.0
15	347.0
16	503.0
17	574.0
18	788.0
19	1072.0
20	1521.0
21	2144.0
22	3177.0
23	4959.0
24	7276.0
25	10876.0
26	16254.0
27	22656.0
28	31779.0
29	42508.0
30	56897.0
31	75975.0
32	103437.0
33	153525.0
34	339642.0
35	711124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.176253708248886	17.051605451780304	11.332812301892933	25.43932853807787
2	17.026847968673273	19.343636474810214	37.25684373890948	26.372671817607035
3	19.538188277087034	23.440470242209518	27.79590661440353	29.225434866299913
4	12.638440955904914	14.947181534611579	35.68439084719344	36.72998666229007
5	15.017246538879526	36.20469042856468	32.9532689672552	15.824794065300592
6	34.54795397386207	35.11068046308383	16.77469268027156	13.566672882782536
7	31.034048268858093	30.22461122073288	20.140227660469886	18.601112849939142
8	28.832406271636952	31.80826486685025	19.30518568639421	20.054143175118583
9	27.670172043173334	14.301671542945272	18.615792086568437	39.41236432731296
10	16.24650107007562	25.828292711200547	30.65884779599703	27.266358422726807
11	37.89846658446953	21.25222319735944	21.720520072251155	19.12879014591987
12	25.57146583301517	23.664878650532938	27.73661602511035	23.02703949134154
13	29.299320113671453	19.092392708392406	25.38735877708676	26.220928400849385
14	24.043162213027422	19.724357629405045	24.8119087988437	31.420571358723837
15	25.43814155260062	26.739442916982014	22.424541850545065	25.3978736798723
16	26.256492199279563	25.72993333110705	23.35062066149706	24.662953808116328
17	24.284607924624133	25.70401789118214	24.85174176253854	25.159632421655186
18	25.35629229198957	24.355649642762636	26.247117529202736	24.04094053604506
19	25.722597071367233	24.567008808832398	25.82649353529613	23.883900584504243
20	26.038760569059587	23.79509570433095	25.071069443919402	25.095074282690057
21	27.52919814034135	23.71801939675133	24.486051615041333	24.266730847865986
22	26.07114212105766	23.88339935268067	25.180810264365117	24.86464826189656
23	24.257316883380366	23.77707503605594	25.891348904964158	26.07425917559953
24	24.64510909323475	24.857895562611333	25.413333148502527	25.083662195651392
25	24.847273096183134	24.292635774926385	25.452194509960098	25.407896618930387
26	24.64619438813479	25.386478163582044	25.74439541286196	24.222932035421206
27	25.487892011638593	24.591874410100218	24.891849081426017	25.028384496835177
28	24.44185705114029	24.482936120157536	26.035498112063955	25.03970871663822
29	24.21278335861038	25.034796629531357	25.886487062080715	24.865932949777548
30	24.347005404397358	25.204791252159424	26.035296800833656	24.41290654260956
31	25.14179182528072	25.035125057806713	24.50210624337664	25.320976873535933
32	24.737914188200264	24.8785417576304	24.619086412239135	25.7644576419302
33	24.30237767154903	24.363367841924568	25.466231418632624	25.86802306789378
34	25.251231347068803	24.769214968593324	25.39986881593464	24.579684868403227
35	26.05097985594375	24.466499770954222	25.357226347006556	24.125294026095464
36	24.47199716701386	25.398010608575326	24.910618531106767	25.21937369330405
37	25.744352810788367	25.25806226006022	24.700636437011262	24.29694849214015
38	24.54036548984523	24.805173940025895	25.471447877846625	25.183012692282247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128.0
1	189.5
2	251.0
3	251.0
4	587.0
5	923.0
6	923.0
7	1220.5
8	1518.0
9	1594.5
10	1671.0
11	1671.0
12	2201.0
13	2731.0
14	3472.0
15	4213.0
16	4213.0
17	5958.5
18	7704.0
19	7704.0
20	9317.0
21	10930.0
22	11089.0
23	11248.0
24	11248.0
25	12886.5
26	14525.0
27	14525.0
28	18852.0
29	23179.0
30	28696.5
31	34214.0
32	34214.0
33	43482.5
34	52751.0
35	52751.0
36	59174.0
37	65597.0
38	76215.5
39	86834.0
40	86834.0
41	92626.5
42	98419.0
43	111233.5
44	124048.0
45	124048.0
46	127935.0
47	131822.0
48	131822.0
49	138030.0
50	144238.0
51	146079.0
52	147920.0
53	147920.0
54	140307.0
55	132694.0
56	132694.0
57	128250.0
58	123806.0
59	110967.5
60	98129.0
61	98129.0
62	94189.5
63	90250.0
64	75852.0
65	61454.0
66	61454.0
67	51979.0
68	42504.0
69	42504.0
70	34838.0
71	27172.0
72	21533.5
73	15895.0
74	15895.0
75	12272.5
76	8650.0
77	8650.0
78	8357.0
79	8064.0
80	6305.0
81	4546.0
82	4546.0
83	4523.5
84	4501.0
85	4501.0
86	3056.5
87	1612.0
88	1390.0
89	1168.0
90	1168.0
91	850.0
92	532.0
93	445.5
94	359.0
95	359.0
96	336.5
97	314.0
98	314.0
99	539.0
100	764.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12354483587626112
2	0.05676384351071457
3	0.011403169451098044
4	0.0030870458735016803
5	1.8900280858173552E-4
6	3.7800561716347104E-4
7	1.260018723878237E-4
8	3.150046809695592E-4
9	1.260018723878237E-4
10	4.410065533573829E-4
11	0.002331034639174738
12	0.002772041192532121
13	0.015624232176090136
14	0.009387139492892865
15	0.025515379158534295
16	0.011403169451098044
17	0.022113328604063058
18	0.006174091747003361
19	0.0076231132794633335
20	0.006174091747003361
21	0.006615098300360744
22	0.0073711095346876854
23	0.010143150727219807
24	0.015183225622732754
25	0.018585276177203992
26	0.020097298645857877
27	0.009072134811923305
28	0.005985088938421625
29	0.012537186302588455
30	0.003276048682083416
31	0.0057960861298398895
32	0.006741100172748568
33	0.008631128258565922
34	0.01228518255781281
35	0.016317242474223167
36	0.016380243410417078
37	0.010143150727219807
38	0.0058590870660338015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1587278.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.15090334381883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.01027178619519	39.90295871960707
2	13.162331918345146	13.465303354690683
3	4.134808346403316	6.344975462162742
4	1.700052734060728	3.4783693231734096
5	0.8289873896670442	2.120172692105183
6	0.48595105941366756	1.4914101401936923
7	0.3094479611774038	1.107997992648903
8	0.2121266116417633	0.8680374246991704
9	0.15248406639337056	0.7019727967403775
>10	0.8123334932421076	7.62226602749189
>50	0.08242507110063504	2.967215466810486
>100	0.08850714308003134	9.866391530368183
>500	0.013968191332958846	4.868112892185234
>1k	0.006180615634052586	4.853759951275451
>5k	1.2361231268105173E-4	0.34105622584745365
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5394	0.3398270498299605	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3309	0.2084700978656543	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2898	0.18257671308995652	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2711	0.170795538021695	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2127	0.13400299128445048	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2099	0.13223896507102095	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2083	0.13123095009191837	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2044	0.1287739135803558	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2017	0.1270728883031202	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1922	0.12108779936469856	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1883	0.118630762853136	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1840	0.1159217225967978	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1839	0.11585872166060389	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1797	0.11321268234045959	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1725	0.10867661493449791	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1614	0.1016835110169737	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1609	0.10136850633600414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	6.300093619391184E-5
3	0.0	0.0	0.0	0.0	6.300093619391184E-5
4	0.0	0.0	0.0	0.0	6.300093619391184E-5
5	0.0	0.0	0.0	0.0	6.300093619391184E-5
6	0.0	0.0	0.0	0.0	6.300093619391184E-5
7	0.0	0.0	0.0	0.0	6.300093619391184E-5
8	0.0	0.0	0.0	0.0	6.300093619391184E-5
9	0.0	0.0	0.0	0.0	6.300093619391184E-5
10	0.0	0.0	0.0	0.0	6.300093619391184E-5
11	0.0	0.0	0.0	0.0	6.300093619391184E-5
12	0.0	0.0	0.0	0.0	1.8900280858173552E-4
13	0.0	0.0	0.0	0.0	3.150046809695592E-4
14	0.0	0.0	0.0	0.0	3.150046809695592E-4
15	0.0	0.0	0.0	0.0	3.7800561716347104E-4
16	0.0	0.0	0.0	6.300093619391184E-5	3.7800561716347104E-4
17	0.0	0.0	0.0	6.300093619391184E-5	3.7800561716347104E-4
18	0.0	0.0	0.0	1.2600187238782367E-4	3.7800561716347104E-4
19	0.0	0.0	0.0	1.2600187238782367E-4	3.7800561716347104E-4
20	0.0	0.0	0.0	1.2600187238782367E-4	3.7800561716347104E-4
21	0.0	0.0	0.0	2.5200374477564734E-4	3.7800561716347104E-4
22	0.0	0.0	0.0	4.410065533573829E-4	4.410065533573829E-4
23	0.0	0.0	0.0	0.0010080149791025894	4.410065533573829E-4
24	0.0	0.0	0.0	0.0018900280858173552	5.040074895512947E-4
25	0.0	0.0	0.0	0.0022680337029808264	5.040074895512947E-4
26	0.0	0.0	0.0	0.0026460393201442976	5.670084257452066E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	580	0.0	20.690062	29
TCTAGCG	590	0.0	20.609276	28
TAGCGGC	600	0.0	19.7331	30
CTCGACG	95	3.7913196E-8	18.52668	13
CGGTCCA	705	0.0	17.702477	10
CATCGTT	455	0.0	17.230022	28
CCGGTCC	790	0.0	16.810457	9
AGCGGCG	725	0.0	16.551527	31
ATCGTTT	495	0.0	16.485174	29
GCCGGTC	760	0.0	16.419825	8
CGCCGGT	745	0.0	16.320927	7
GCGGCGC	730	0.0	16.218987	32
TCTTGCG	770	0.0	16.212198	2
GTGTACG	60	0.0024313699	16.010908	1
TCGACGC	110	2.0201333E-7	16.000315	14
GTGTAGG	235	0.0	15.670252	1
TCCTACA	195	0.0	15.593983	2
CTTGCGC	835	0.0	15.519796	3
AACCGCG	290	0.0	15.44712	7
ACCTCTA	825	0.0	15.321031	25
>>END_MODULE