FastQCFastQC Report
Thu 2 Feb 2017
SRR4062944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587278
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT62600.39438586057388814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT42740.2692660012927792No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28190.17759963913063748No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26940.16972452210639852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT25860.16292042099745602No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22980.1447761513736094No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22090.13916906805235127No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21910.13803505120086085No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20890.13160895570908185No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19800.12474185366394544No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19480.12272582370574027No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.12152880591805594No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18920.1191977712788812No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18710.11787475161880905No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18380.11579572072440997No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17970.11321268234045959No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17720.11163765893561178No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17450.10993663365837615No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17370.10943262616882486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5250.022.85579328
CTAGCGG5350.022.12953429
TAGCGGC5550.021.90861730
TACCGTA400.00448084820.00267
AGCGGCG6250.019.96682231
AGCGTTC1500.018.1362618
CGCTTCG4400.017.8171332
AAACGCT4650.017.5473529
GCGGCGC7050.017.24720832
TAAACGC5050.017.107928
ACCGTAT753.230467E-517.0694228
CGTCTTA854.989144E-616.94017615
GTCCTAC1354.4383341E-1016.5937021
CGAACGA2800.016.5704516
ATAACGA2900.016.55126812
GTAAACG4800.016.33288427
CGCCGGT7500.016.215447
CGGTCCA7700.016.20887810
CGCGGTT5950.016.13553410
ACGGGTC703.692963E-416.0025849
Produced by FastQC (version 0.11.5)