##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587278
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195977642227763	32.0	32.0	32.0	32.0	32.0
2	31.28602866038589	32.0	32.0	32.0	32.0	32.0
3	31.372639827427836	32.0	32.0	32.0	32.0	32.0
4	31.47302047908432	32.0	32.0	32.0	32.0	32.0
5	31.388851858338615	32.0	32.0	32.0	32.0	32.0
6	34.90917911040158	36.0	36.0	36.0	36.0	36.0
7	34.93377782593849	36.0	36.0	36.0	36.0	36.0
8	34.87758351089097	36.0	36.0	36.0	32.0	36.0
9	34.97592230220541	36.0	36.0	36.0	36.0	36.0
10	34.82904002953484	36.0	36.0	36.0	32.0	36.0
11	35.01195379763343	36.0	36.0	36.0	36.0	36.0
12	34.89586134249955	36.0	36.0	36.0	32.0	36.0
13	34.955629700657354	36.0	36.0	36.0	36.0	36.0
14	34.894879787913645	36.0	36.0	36.0	32.0	36.0
15	34.87295294208072	36.0	36.0	36.0	32.0	36.0
16	34.87974696303987	36.0	36.0	36.0	32.0	36.0
17	34.83956244589795	36.0	36.0	36.0	32.0	36.0
18	34.83788914103263	36.0	36.0	36.0	32.0	36.0
19	34.83593863204807	36.0	36.0	36.0	32.0	36.0
20	34.8174239169194	36.0	36.0	36.0	32.0	36.0
21	34.81108791276638	36.0	36.0	36.0	32.0	36.0
22	34.79420681191323	36.0	36.0	36.0	32.0	36.0
23	34.73821346985216	36.0	36.0	36.0	32.0	36.0
24	34.715071335960054	36.0	36.0	36.0	32.0	36.0
25	34.69112594013147	36.0	36.0	36.0	32.0	36.0
26	34.632256605333154	36.0	36.0	36.0	32.0	36.0
27	34.62810043357244	36.0	36.0	36.0	32.0	36.0
28	34.59000880753088	36.0	36.0	36.0	32.0	36.0
29	34.55901990703582	36.0	36.0	36.0	32.0	36.0
30	34.53801098484323	36.0	36.0	36.0	32.0	36.0
31	34.525282275694614	36.0	36.0	36.0	32.0	36.0
32	34.49975429634885	36.0	36.0	36.0	32.0	36.0
33	34.4571467632009	36.0	36.0	36.0	32.0	36.0
34	34.444542165896586	36.0	36.0	36.0	32.0	36.0
35	34.4064889704261	36.0	36.0	36.0	32.0	36.0
36	34.38157777024566	36.0	36.0	36.0	32.0	36.0
37	34.374315022321234	36.0	36.0	36.0	32.0	36.0
38	33.934214422426315	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	9.0
21	28.0
22	141.0
23	387.0
24	1028.0
25	2597.0
26	5610.0
27	10536.0
28	18743.0
29	29463.0
30	45020.0
31	65367.0
32	92706.0
33	148669.0
34	362596.0
35	804377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40314531982459	18.293764806693886	12.047041181511165	26.256048691970364
2	15.987747577929007	20.55764648662679	36.41851017906126	27.036095756382938
3	18.367229937036864	24.134461638099943	28.237019602111285	29.261288822751908
4	12.13545991429331	15.8462244073802	35.739324149453715	36.27899152887277
5	14.431813456747966	36.6850671400977	32.7248282909484	16.158291112205927
6	34.43917055278421	35.63014325188972	16.396853977322127	13.53383221800395
7	30.628127612578304	30.469301752124554	20.18379951022918	18.718771125067963
8	28.625844545640117	32.19712911184418	19.041810836897163	20.135215505618547
9	27.58837508788996	14.121932041844442	18.368623725123044	39.92106914514255
10	16.125735857425102	26.129301741865245	30.55893663561953	27.186025765090115
11	37.95555180330595	21.22901015375543	21.631795947066397	19.183642095872216
12	25.283253279841134	23.838699353355945	27.7064044473789	23.171642919424016
13	29.44268706722267	19.0591812267172	25.24594005961152	26.2521916464486
14	23.857728586584816	19.70911171088078	24.833425314816076	31.599734387718332
15	25.354852773112206	26.979646917553197	22.260435790075842	25.405064519258758
16	26.169219361207908	25.84356731685774	23.219324667339098	24.76788865459526
17	24.216488857024416	25.84210201363592	24.777701196639782	25.16370793269988
18	25.301112974538803	24.35477591197005	26.21531955964866	24.12879155384249
19	25.62796183151282	24.64993529803853	25.73852847453313	23.983574395915525
20	26.053092148949336	23.70183420925635	25.126411378473083	25.118662263321234
21	27.383975681592666	23.8875431163472	24.466900820588744	24.26158038147139
22	25.92271287413153	24.008553017874647	25.25591012527122	24.812823982722602
23	24.123230064103574	23.702824022302373	25.99965349419603	26.174292419398025
24	24.613457755982253	24.827975943722524	25.474365549072058	25.08420075122316
25	24.80619324793341	24.237546439594347	25.58513731377699	25.37112299869525
26	24.71578684753827	25.25740623722261	25.778361306816645	24.248445608422475
27	25.60506175359547	24.439668967463756	24.875761997283398	25.07950728165738
28	24.37147116006144	24.500182072705602	26.187725149595725	24.940621617637238
29	24.29038895517987	24.816761777080007	26.010440515146055	24.882408752594063
30	24.376259231212174	24.955426837642808	26.15338963937004	24.514924291774975
31	25.139515573201415	24.826715924998645	24.703990101292906	25.32977840050703
32	24.907546126135436	24.657558411317993	24.829424965254983	25.605470497291588
33	24.373550190955836	24.148006839381633	25.617314673296043	25.86112829636648
34	25.279251649679512	24.507868186921257	25.618826695764703	24.59405346763453
35	26.062605290314618	24.28900293458361	25.40834056794084	24.24005120716094
36	24.46357852877694	25.19735043262743	25.157596841889067	25.18147419670656
37	25.816208628860227	24.961663930326004	24.8944419314071	24.327685509406667
38	24.659274972169317	24.50901764468332	25.5465807165353	25.28512666661207
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	68.5
2	93.0
3	93.0
4	415.5
5	738.0
6	738.0
7	1117.0
8	1496.0
9	1606.0
10	1716.0
11	1716.0
12	2236.0
13	2756.0
14	3558.0
15	4360.0
16	4360.0
17	6428.5
18	8497.0
19	8497.0
20	9977.0
21	11457.0
22	11544.5
23	11632.0
24	11632.0
25	12922.0
26	14212.0
27	14212.0
28	18493.0
29	22774.0
30	28526.0
31	34278.0
32	34278.0
33	43873.5
34	53469.0
35	53469.0
36	59708.5
37	65948.0
38	76215.0
39	86482.0
40	86482.0
41	92326.0
42	98170.0
43	111699.0
44	125228.0
45	125228.0
46	128224.5
47	131221.0
48	131221.0
49	138522.0
50	145823.0
51	146678.0
52	147533.0
53	147533.0
54	139703.0
55	131873.0
56	131873.0
57	128017.0
58	124161.0
59	111316.5
60	98472.0
61	98472.0
62	94540.5
63	90609.0
64	76195.0
65	61781.0
66	61781.0
67	52530.5
68	43280.0
69	43280.0
70	35208.5
71	27137.0
72	21358.5
73	15580.0
74	15580.0
75	11949.0
76	8318.0
77	8318.0
78	8042.0
79	7766.0
80	6057.5
81	4349.0
82	4349.0
83	4135.0
84	3921.0
85	3921.0
86	2533.5
87	1146.0
88	935.5
89	725.0
90	725.0
91	434.0
92	143.0
93	92.5
94	42.0
95	42.0
96	32.0
97	22.0
98	22.0
99	24.0
100	26.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00995414791863807
2	0.0
3	0.0
4	2.520037447756474E-4
5	0.0
6	2.520037447756474E-4
7	3.150046809695592E-4
8	0.0021420318305930028
9	0.0041580617887981815
10	8.820131067147658E-4
11	0.005166076767900771
12	3.7800561716347104E-4
13	6.300093619391185E-5
14	1.260018723878237E-4
15	0.0
16	6.300093619391185E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.8900280858173552E-4
22	1.260018723878237E-4
23	1.8900280858173552E-4
24	0.0
25	6.300093619391185E-5
26	6.300093619391185E-5
27	1.260018723878237E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.300093619391185E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1587278.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.036439644779136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23373046643664	37.3823072291378
2	14.204594568125648	13.930854884369017
3	4.525765102574201	6.657822218964824
4	1.8246894365546322	3.579050937043091
5	0.9063971644732074	2.222324492494469
6	0.5184577295579226	1.5253992698301777
7	0.3226642259182186	1.1075613387837673
8	0.22738442922581614	0.8920098271915431
9	0.1666790980839706	0.7356014579916763
>10	0.8663231103504208	7.784660660833631
>50	0.08566125281906657	2.9801661581331724
>100	0.09541574634094431	10.26877035568453
>500	0.015385364390646642	5.2878880980540615
>1k	0.006723016372383405	5.248707755341849
>5k	1.2928877639198857E-4	0.39687531614634763
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6260	0.39438586057388814	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4274	0.2692660012927792	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2819	0.17759963913063748	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2694	0.16972452210639852	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2586	0.16292042099745602	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2298	0.1447761513736094	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2209	0.13916906805235127	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2191	0.13803505120086085	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2089	0.13160895570908185	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1980	0.12474185366394544	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1948	0.12272582370574027	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1929	0.12152880591805594	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1892	0.1191977712788812	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1871	0.11787475161880905	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1838	0.11579572072440997	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1797	0.11321268234045959	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1772	0.11163765893561178	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1745	0.10993663365837615	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1737	0.10943262616882486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.300093619391184E-5
2	0.0	0.0	0.0	0.0	6.300093619391184E-5
3	0.0	0.0	0.0	0.0	6.300093619391184E-5
4	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
5	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
6	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
7	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
8	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
9	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
10	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
11	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
12	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
13	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
14	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
15	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
16	0.0	0.0	0.0	6.300093619391184E-5	6.300093619391184E-5
17	0.0	0.0	0.0	1.2600187238782367E-4	6.300093619391184E-5
18	0.0	0.0	0.0	1.8900280858173552E-4	1.2600187238782367E-4
19	0.0	0.0	0.0	1.8900280858173552E-4	1.2600187238782367E-4
20	0.0	0.0	0.0	1.8900280858173552E-4	1.2600187238782367E-4
21	0.0	0.0	0.0	3.150046809695592E-4	1.2600187238782367E-4
22	0.0	0.0	0.0	4.410065533573829E-4	1.2600187238782367E-4
23	0.0	0.0	0.0	9.450140429086776E-4	1.2600187238782367E-4
24	0.0	0.0	0.0	0.0018270271496234434	1.2600187238782367E-4
25	0.0	0.0	0.0	0.0022680337029808264	1.2600187238782367E-4
26	0.0	0.0	0.0	0.0026460393201442976	1.2600187238782367E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	525	0.0	22.855793	28
CTAGCGG	535	0.0	22.129534	29
TAGCGGC	555	0.0	21.908617	30
TACCGTA	40	0.004480848	20.0026	7
AGCGGCG	625	0.0	19.966822	31
AGCGTTC	150	0.0	18.136261	8
CGCTTCG	440	0.0	17.81713	32
AAACGCT	465	0.0	17.54735	29
GCGGCGC	705	0.0	17.247208	32
TAAACGC	505	0.0	17.1079	28
ACCGTAT	75	3.230467E-5	17.069422	8
CGTCTTA	85	4.989144E-6	16.940176	15
GTCCTAC	135	4.4383341E-10	16.593702	1
CGAACGA	280	0.0	16.57045	16
ATAACGA	290	0.0	16.551268	12
GTAAACG	480	0.0	16.332884	27
CGCCGGT	750	0.0	16.21544	7
CGGTCCA	770	0.0	16.208878	10
CGCGGTT	595	0.0	16.135534	10
ACGGGTC	70	3.692963E-4	16.002584	9
>>END_MODULE