FastQCFastQC Report
Thu 2 Feb 2017
SRR4062943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953098
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30040.31518269894596357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17390.18245762765214069No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15190.15937500655756281No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14990.15727658645805573No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14570.15286990424909086No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14280.14982719510480558No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14040.14730909098539707No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13200.13849572656746734No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13000.13639730646796028No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12680.13303983430874894No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12570.13188570325402005No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12190.1278987050649566No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11760.12338710185101638No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11120.11667215753259372No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11070.11614755250771694No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10910.11446881642811128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10890.11425897441816057No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT10490.1100621342191464No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA10210.1071243460798365No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10000.10492100497535406No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9930.10418655794052659No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9840.1032422688957484No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9560.10030448075643847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCG400.004484714519.99862324
TATACCT507.203121E-419.1966655
GACCTAT450.0087780417.802711
CTATTAT652.0775596E-417.2549321
CCTATTC2750.016.8742223
GTCCTAT3100.016.539291
CATCGTT3100.016.51585828
ATCGTTT3050.016.26288229
GTGCTAA1001.2947239E-616.0224381
CAACCGG600.002442616515.998898513
CTATTCC3000.015.99889854
TAGGACA703.6975084E-415.99889854
TCTAGCG2850.015.71904128
AAACGCT3100.015.48442929
CTAGCGG2900.015.44883329
CATGCAC3700.015.1364763
ATAATAG859.422378E-515.0601573
GGACCGT756.251201E-414.9307396
ACCGTCG2150.014.8826968
ACTAAGG650.004158436314.7705383
Produced by FastQC (version 0.11.5)