##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062943_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	953098
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20875817596931	32.0	32.0	32.0	32.0	32.0
2	30.857316876123967	32.0	32.0	32.0	32.0	32.0
3	30.91741772619395	32.0	32.0	32.0	32.0	32.0
4	30.960350352219812	32.0	32.0	32.0	32.0	32.0
5	30.90617229288069	32.0	32.0	32.0	32.0	32.0
6	34.540118644672425	36.0	36.0	36.0	32.0	36.0
7	34.45406558402179	36.0	36.0	36.0	32.0	36.0
8	34.444592266482566	36.0	36.0	36.0	32.0	36.0
9	34.55079540613872	36.0	36.0	36.0	32.0	36.0
10	34.31709750728676	36.0	36.0	36.0	32.0	36.0
11	34.555179005726586	36.0	36.0	36.0	32.0	36.0
12	34.381153879244316	36.0	36.0	36.0	32.0	36.0
13	34.45995165240091	36.0	36.0	36.0	32.0	36.0
14	34.365188049917215	36.0	36.0	36.0	32.0	36.0
15	34.323465163078716	36.0	36.0	36.0	32.0	36.0
16	34.328399597942706	36.0	36.0	36.0	32.0	36.0
17	34.25434110658085	36.0	36.0	36.0	32.0	36.0
18	34.281360363782106	36.0	36.0	36.0	32.0	36.0
19	34.2684183578184	36.0	36.0	36.0	32.0	36.0
20	34.23623279033216	36.0	36.0	36.0	32.0	36.0
21	34.22141584600954	36.0	36.0	36.0	32.0	36.0
22	34.18726091125991	36.0	36.0	36.0	32.0	36.0
23	34.157235667266114	36.0	36.0	36.0	32.0	36.0
24	34.131383131640185	36.0	36.0	36.0	32.0	36.0
25	34.11396729402433	36.0	36.0	36.0	32.0	36.0
26	34.057988790239826	36.0	36.0	36.0	32.0	36.0
27	34.062928471154066	36.0	36.0	36.0	32.0	36.0
28	34.04925936262588	36.0	36.0	36.0	32.0	36.0
29	34.02650409506683	36.0	36.0	36.0	32.0	36.0
30	33.986811429674596	36.0	36.0	36.0	32.0	36.0
31	34.01327040870928	36.0	36.0	36.0	32.0	36.0
32	33.95525328979811	36.0	36.0	36.0	32.0	36.0
33	33.90896319161303	36.0	36.0	36.0	32.0	36.0
34	33.927625490768	36.0	36.0	36.0	32.0	36.0
35	33.87405597325773	36.0	36.0	36.0	32.0	36.0
36	33.83600322317327	36.0	36.0	36.0	32.0	36.0
37	33.830279782351866	36.0	36.0	36.0	32.0	36.0
38	33.214615915677086	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	1.0
9	3.0
10	9.0
11	11.0
12	5.0
13	8.0
14	172.0
15	293.0
16	363.0
17	419.0
18	541.0
19	709.0
20	1001.0
21	1374.0
22	2059.0
23	2901.0
24	4548.0
25	6557.0
26	9526.0
27	13403.0
28	18731.0
29	25205.0
30	33833.0
31	45154.0
32	60583.0
33	89432.0
34	200406.0
35	435848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.231307809881976	16.783919070104577	11.037308219573816	25.94746490043963
2	17.38139078840457	19.745491726696493	36.227012477665966	26.646105007232972
3	19.160322565412887	24.080651846566315	27.850910559977333	28.90811502804346
4	12.952435513249897	15.185653849059456	35.11065363920996	36.75125699848069
5	15.272680344226277	35.79311169727225	32.68103670781686	16.253171250684613
6	34.46732074489795	34.805061814274936	16.781188063754264	13.946429377072848
7	30.82967333894311	29.999328505568158	20.645830754025294	18.525167401463438
8	28.898903047440182	31.12302551162266	19.53488372093023	20.443187720006925
9	28.154305871194236	14.069546130811862	18.194008146101016	39.58213985189289
10	16.557968049321474	25.427502428931458	30.393854121419295	27.620675400327777
11	37.90432242549387	20.887841053599082	21.76332212751134	19.4445143933957
12	25.99315894950004	23.27677925021247	27.433766669814393	23.2962951304731
13	29.868966031067444	18.663552157018298	24.87001014734107	26.597471664573185
14	24.796643889663045	19.300047848496547	24.16873730335947	31.734570958480933
15	25.936999544539617	25.766464263151157	22.24986934373826	26.046666848570965
16	26.829670110578753	24.86038619664927	22.84577108163184	25.46417261114014
17	24.679300169164964	24.97397462105789	24.506462269496996	25.84026294028015
18	26.09591182760046	23.54607666818461	25.517028630427617	24.840982873787304
19	26.36133458269894	24.1632625650959	24.945200347947623	24.53020250425753
20	26.429243714055772	23.116157890705416	24.42638758749642	26.02821080774239
21	28.066738577508417	23.341398819766805	23.583783125855167	25.00807947686961
22	26.786117135748995	23.29703860629927	24.191553055461995	25.725291202489743
23	24.8649783052377	23.1645496566089	24.903698340494966	27.066773697658437
24	25.368824117671135	24.452836999625376	24.4658490746211	25.71248980808239
25	25.69763049090184	23.42319558419208	24.577937750540432	26.30123617436565
26	25.393968713369592	24.518459363794438	25.00726705291359	25.080304869922376
27	26.14474139830642	23.980021196000042	24.07928563184017	25.79595177385337
28	25.143908821720945	23.589504676643223	25.23635043450706	26.03023606712877
29	24.945409749540666	24.184136771006358	25.172480008058685	25.697973471394285
30	25.05833815987174	24.29994208153912	25.235556059194348	25.406163699394796
31	25.734210114886086	24.3374035052375	23.65883166444743	26.269554715428985
32	25.253297909400352	24.225101466063954	23.75229269234482	26.769307932190873
33	24.8306950370089	23.66596923025265	24.67739193757909	26.825943795159358
34	25.90605829749815	23.833493883358834	24.716309154289622	25.544138664853396
35	26.915371982349267	23.572226857025925	24.589714933337532	24.922686227287276
36	25.20215831414762	24.621235175231856	24.106316489780326	26.070290020840197
37	26.445540398740818	24.490766002098635	24.06232948583421	25.001364113326336
38	25.150492267367863	24.220624759322547	24.75355153356788	25.87533143974171
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	421.0
1	343.0
2	265.0
3	265.0
4	567.0
5	869.0
6	869.0
7	981.0
8	1093.0
9	1062.0
10	1031.0
11	1031.0
12	1312.0
13	1593.0
14	1939.0
15	2285.0
16	2285.0
17	3125.5
18	3966.0
19	3966.0
20	4748.0
21	5530.0
22	5334.5
23	5139.0
24	5139.0
25	5768.0
26	6397.0
27	6397.0
28	8239.0
29	10081.0
30	12786.0
31	15491.0
32	15491.0
33	20959.0
34	26427.0
35	26427.0
36	29644.5
37	32862.0
38	38895.0
39	44928.0
40	44928.0
41	48223.5
42	51519.0
43	60716.5
44	69914.0
45	69914.0
46	73938.5
47	77963.0
48	77963.0
49	84059.5
50	90156.0
51	91302.0
52	92448.0
53	92448.0
54	88928.0
55	85408.0
56	85408.0
57	84640.0
58	83872.0
59	75592.5
60	67313.0
61	67313.0
62	65060.5
63	62808.0
64	52165.0
65	41522.0
66	41522.0
67	34411.5
68	27301.0
69	27301.0
70	22032.0
71	16763.0
72	13186.5
73	9610.0
74	9610.0
75	7212.5
76	4815.0
77	4815.0
78	4837.0
79	4859.0
80	3758.5
81	2658.0
82	2658.0
83	2823.5
84	2989.0
85	2989.0
86	2017.0
87	1045.0
88	868.5
89	692.0
90	692.0
91	489.0
92	286.0
93	241.0
94	196.0
95	196.0
96	174.0
97	152.0
98	152.0
99	291.5
100	431.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12202312878633677
2	0.05413923856728269
3	0.011856073562215009
4	0.003567314169162038
5	4.1968401990141626E-4
6	1.0492100497535407E-4
7	0.0
8	3.1476301492606214E-4
9	4.1968401990141626E-4
10	4.1968401990141626E-4
11	0.0023082621094577893
12	0.0029377881393099136
13	0.014584019691574215
14	0.009442890447781864
15	0.022662937074676474
16	0.011751152557239655
17	0.01909562290551444
18	0.005875576278619827
19	0.008498601403003679
20	0.0061903392935458885
21	0.006924786328373368
22	0.0070297073333487215
23	0.010806863512461467
24	0.014374177681623506
25	0.01657751878610594
26	0.01752180783088413
27	0.009233048437831157
28	0.00671494431842266
29	0.009337969442806512
30	0.003567314169162038
31	0.0070297073333487215
32	0.0073444703482747845
33	0.00881336441792974
34	0.013010204616943903
35	0.017102123810982712
36	0.013849572656746736
37	0.010282258487584697
38	0.0061903392935458885
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	953098.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.58953163077852
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62009542805818	42.39773058590248
2	11.821069997214861	12.433290690563558
3	3.3869010439914686	5.343466187499184
4	1.360380515851754	2.861670966731224
5	0.7014685044678043	1.8444950051852247
6	0.435695866031146	1.3747824916826636
7	0.2832042317700991	1.0425504533250771
8	0.20577378123614892	0.8657237421682683
9	0.14208315859096557	0.6724878086628454
>10	0.8189376781231706	7.980231572072741
>50	0.09697076776309846	3.5463309233755687
>100	0.11139147637783053	12.488235828756657
>500	0.012221007274131794	4.403423024477178
>1k	0.0038065432493197392	2.745580719597329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3004	0.31518269894596357	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1739	0.18245762765214069	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1519	0.15937500655756281	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1499	0.15727658645805573	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1457	0.15286990424909086	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1428	0.14982719510480558	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1404	0.14730909098539707	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1320	0.13849572656746734	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1300	0.13639730646796028	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1268	0.13303983430874894	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1257	0.13188570325402005	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1219	0.1278987050649566	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1176	0.12338710185101638	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1112	0.11667215753259372	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1107	0.11614755250771694	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1091	0.11446881642811128	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1089	0.11425897441816057	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1049	0.1100621342191464	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1021	0.1071243460798365	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1000	0.10492100497535406	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	993	0.10418655794052659	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	984	0.1032422688957484	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	956	0.10030448075643847	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.196840199014162E-4
2	0.0	0.0	0.0	0.0	4.196840199014162E-4
3	0.0	0.0	0.0	0.0	4.196840199014162E-4
4	0.0	0.0	0.0	0.0	4.196840199014162E-4
5	0.0	0.0	0.0	0.0	4.196840199014162E-4
6	0.0	0.0	0.0	0.0	5.246050248767703E-4
7	0.0	0.0	0.0	0.0	5.246050248767703E-4
8	0.0	0.0	0.0	0.0	5.246050248767703E-4
9	0.0	0.0	0.0	0.0	5.246050248767703E-4
10	0.0	0.0	0.0	0.0	6.295260298521243E-4
11	0.0	0.0	0.0	0.0	6.295260298521243E-4
12	0.0	0.0	0.0	0.0	0.0010492100497535406
13	0.0	0.0	0.0	0.0	0.001573815074630311
14	0.0	0.0	0.0	0.0	0.001573815074630311
15	0.0	0.0	0.0	0.0	0.001888578089556373
16	0.0	0.0	0.0	1.0492100497535405E-4	0.001888578089556373
17	0.0	0.0	0.0	1.0492100497535405E-4	0.001888578089556373
18	0.0	0.0	0.0	1.0492100497535405E-4	0.001993499094531727
19	0.0	0.0	0.0	1.0492100497535405E-4	0.001993499094531727
20	0.0	0.0	0.0	1.0492100497535405E-4	0.002203341104482435
21	0.0	0.0	0.0	3.1476301492606214E-4	0.002203341104482435
22	0.0	0.0	0.0	4.196840199014162E-4	0.0024131831144331433
23	0.0	0.0	0.0	0.0010492100497535406	0.0024131831144331433
24	0.0	0.0	0.0	0.003147630149260622	0.0024131831144331433
25	0.0	0.0	0.0	0.003567314169162038	0.0024131831144331433
26	0.0	0.0	0.0	0.004196840199014162	0.0026230251243838515
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACCG	40	0.0044847145	19.998623	24
TATACCT	50	7.203121E-4	19.196665	5
GACCTAT	45	0.00877804	17.80271	1
CTATTAT	65	2.0775596E-4	17.254932	1
CCTATTC	275	0.0	16.874222	3
GTCCTAT	310	0.0	16.53929	1
CATCGTT	310	0.0	16.515858	28
ATCGTTT	305	0.0	16.262882	29
GTGCTAA	100	1.2947239E-6	16.022438	1
CAACCGG	60	0.0024426165	15.9988985	13
CTATTCC	300	0.0	15.9988985	4
TAGGACA	70	3.6975084E-4	15.9988985	4
TCTAGCG	285	0.0	15.719041	28
AAACGCT	310	0.0	15.484429	29
CTAGCGG	290	0.0	15.448833	29
CATGCAC	370	0.0	15.136476	3
ATAATAG	85	9.422378E-5	15.060157	3
GGACCGT	75	6.251201E-4	14.930739	6
ACCGTCG	215	0.0	14.882696	8
ACTAAGG	65	0.0041584363	14.770538	3
>>END_MODULE