FastQCFastQC Report
Thu 2 Feb 2017
SRR4062943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953098
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37070.3889421654436375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23540.24698404571198346No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16970.17805094544317582No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15590.16357184675657696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15100.15843071751278465No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14670.15391911429884442No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14550.15266006223914017No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13830.14510574988091465No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13780.1445811448560379No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13640.14311225078638293No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13610.14279748777145687No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13090.13734159551273845No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12460.13073157219929116No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12220.12821346807988265No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11680.12254773381121353No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.12023947170175574No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11250.1180361305972733No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11140.11688199954254441No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11040.11583278949279087No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10910.11446881642811128No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10690.11216055431865349No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT10180.10680958306491042No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT9930.10418655794052659No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9820.1030324268857977No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT9630.10103892779126594No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9560.10030448075643847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT308.4117753E-426.6688527
ACGCGTA308.4117753E-426.6688526
TACGCGT350.002065298422.8590185
ATGATCG400.004480800620.001645
ATACGCG400.004483522419.9995424
GTTAATA507.195813E-419.199562
GTATAGA852.395518E-718.8230971
GTTTATA802.7957776E-617.9995881
TAATACC1006.812297E-817.5995984
ACCGTCG2600.017.2321838
TGTAATA652.100297E-417.2303752
TTATCCG753.2333926E-517.06627527
CGTCTTA957.690851E-716.8417215
TACCGTC2800.016.5727887
CGATAAC2050.016.39158810
TAACGAA2150.016.37171713
AACCGCG1900.016.0013127
CGTCGTA2800.016.00131210
GATAACG2100.016.0013111
CCGATAA2100.016.001319
Produced by FastQC (version 0.11.5)