FastQCFastQC Report
Thu 2 Feb 2017
SRR4062942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506624
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT38850.2578612845673506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24000.15929654645087293No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23400.15531413278960113No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22320.14814578819931185No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG21350.1417075527802557No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21320.14150843209719213No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20290.13467195531200882No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20170.13387547257975446No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19850.13175151862707615No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19440.1290302026252071No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18720.1242513062316809No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18560.12318932925534175No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18450.12245922008410857No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15780.10473747929144897No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15660.1039409965591946No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15220.10102055987426192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4550.017.23032428
CGAGCCG5600.016.28583315
ACGTAAC600.002425534216.0165981
CTAGCGG4900.015.99958529
AGGCCCG6350.015.37019110
TAGGACG2950.015.1835274
TACCGTC3300.015.0279187
GATATAC3200.015.0155591
TAGCGGC5150.014.91223430
CGCAAGA5400.014.8203342
GCGCAAG5300.014.8077981
CGTAACA650.004152083314.7747342
CAAGACG5650.014.7228374
AAGACGG5550.014.6993945
TATACAC4550.014.416733
ACCGTCG3700.014.2680068
TGGATAC6550.014.16757324
AGAGCGA5900.014.10179715
ATACCGT3800.013.891616
GAGCCGC6800.013.88245316
Produced by FastQC (version 0.11.5)