##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062942_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1506624
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229864252793	32.0	32.0	32.0	32.0	32.0
2	30.87234041140988	32.0	32.0	32.0	32.0	32.0
3	30.918156753111592	32.0	32.0	32.0	32.0	32.0
4	30.975474305467056	32.0	32.0	32.0	32.0	32.0
5	30.919399266173908	32.0	32.0	32.0	32.0	32.0
6	34.55581020878467	36.0	36.0	36.0	32.0	36.0
7	34.47677788220551	36.0	36.0	36.0	32.0	36.0
8	34.46897898878552	36.0	36.0	36.0	32.0	36.0
9	34.581809396372286	36.0	36.0	36.0	32.0	36.0
10	34.32285958540419	36.0	36.0	36.0	32.0	36.0
11	34.56141213733486	36.0	36.0	36.0	32.0	36.0
12	34.401100075400365	36.0	36.0	36.0	32.0	36.0
13	34.475470986789006	36.0	36.0	36.0	32.0	36.0
14	34.38371152988403	36.0	36.0	36.0	32.0	36.0
15	34.32638136655197	36.0	36.0	36.0	32.0	36.0
16	34.35402661845291	36.0	36.0	36.0	32.0	36.0
17	34.27499561934497	36.0	36.0	36.0	32.0	36.0
18	34.2890887175566	36.0	36.0	36.0	32.0	36.0
19	34.289305095365535	36.0	36.0	36.0	32.0	36.0
20	34.270296371224674	36.0	36.0	36.0	32.0	36.0
21	34.244846092986705	36.0	36.0	36.0	32.0	36.0
22	34.22398023660847	36.0	36.0	36.0	32.0	36.0
23	34.177173601376325	36.0	36.0	36.0	32.0	36.0
24	34.163479408266426	36.0	36.0	36.0	32.0	36.0
25	34.14087589206066	36.0	36.0	36.0	32.0	36.0
26	34.089668689732804	36.0	36.0	36.0	32.0	36.0
27	34.08766354445436	36.0	36.0	36.0	32.0	36.0
28	34.08103946306444	36.0	36.0	36.0	32.0	36.0
29	34.0385424631494	36.0	36.0	36.0	32.0	36.0
30	34.016611311116776	36.0	36.0	36.0	32.0	36.0
31	34.0391212406015	36.0	36.0	36.0	32.0	36.0
32	33.991847999235375	36.0	36.0	36.0	32.0	36.0
33	33.9519422231426	36.0	36.0	36.0	32.0	36.0
34	33.95982939339875	36.0	36.0	36.0	32.0	36.0
35	33.919233332271354	36.0	36.0	36.0	32.0	36.0
36	33.872260099401046	36.0	36.0	36.0	32.0	36.0
37	33.88054683849454	36.0	36.0	36.0	32.0	36.0
38	33.287108794231344	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	5.0
10	11.0
11	19.0
12	8.0
13	6.0
14	261.0
15	525.0
16	595.0
17	729.0
18	859.0
19	1161.0
20	1484.0
21	2056.0
22	3002.0
23	4491.0
24	6820.0
25	10072.0
26	14546.0
27	20854.0
28	29170.0
29	39106.0
30	51930.0
31	69525.0
32	96027.0
33	142411.0
34	317707.0
35	693236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.427994814076946	17.404672779818227	11.436674789330183	25.73065761677464
2	16.789989161964872	19.77662490835485	37.400983392304994	26.032402537375283
3	19.056444707022564	23.914486567580305	28.378683377256163	28.650385348140965
4	12.586935887479505	15.314323264103228	36.1626741538727	35.93606669454456
5	14.89331763375286	36.20018823566893	33.31041229983367	15.59608183074454
6	34.08944132524547	35.532871947054964	16.897038999242675	13.480647728456896
7	30.434733549976638	30.398294464976	20.702643791682597	18.464328193364768
8	28.224871865330165	32.43410074750202	19.568530310934822	19.77249707623299
9	27.645506069542375	14.363134457836443	18.722425878837477	39.26893359378371
10	16.189383234093334	26.155220603511044	30.943431542322962	26.711964620072653
11	37.59874152470653	21.28774679158494	22.251383094880143	18.862128588828377
12	25.45178303794141	23.56287659649034	28.1352010439497	22.850139321618546
13	29.372755014727037	19.18617271624714	25.360774236547346	26.080298032478478
14	23.907294439392228	20.0506475316796	24.859308724253065	31.182749304675106
15	25.274286086064023	26.859057515432117	22.379460841785757	25.4871955567181
16	25.997105797697916	25.781235479202902	23.51638941624736	24.705269306851825
17	24.1086958253559	25.720292737590384	25.039964629311466	25.13104680774225
18	25.168448337089167	24.604641746512716	26.204394823918303	24.022515092479814
19	25.820288308165946	24.748077182208185	25.47439019776132	23.95724431186455
20	25.91190560110133	24.101801276166697	24.893282169841815	25.09301095289016
21	27.295893136996547	24.01350921689422	24.34008816303989	24.350509483069338
22	26.014748023075896	24.01548748196988	25.005194134511154	24.964570360443066
23	24.328280236765575	23.802082883882775	25.641881777852717	26.227755101498936
24	24.742068207375702	25.111955209950036	25.219761472006887	24.92621511066738
25	24.981760801289987	24.405408760524466	25.24185608990083	25.37097434828472
26	24.6037557482386	25.48224792558873	25.808139114178758	24.10585721199391
27	25.425110405571623	24.552420160492165	24.798289535085175	25.22417989885104
28	24.44379247309401	24.671001538622885	25.723610758943327	25.161595229339774
29	24.447989209046188	24.843091061285264	25.611123391662634	25.097796338005917
30	24.34390702058318	25.098070451422767	25.929495476479687	24.628527051514368
31	25.219544250695307	24.883142054920913	24.50936921269407	25.38794448168971
32	24.80289036602187	24.78662774257528	24.48752822726784	25.922953664135008
33	24.359476456055155	24.282476890173395	25.435744526061033	25.922302127710417
34	25.389477066175086	24.595288371348932	25.4089268561771	24.606307706298878
35	26.001690145707023	24.43966624601113	25.435431587933717	24.123212020348134
36	24.478856185417133	25.28295974901719	24.847749399561074	25.3904346660046
37	25.660512372534157	24.97779595109666	24.76126498690326	24.60042668946592
38	24.5808439077913	24.796902038380686	25.469702410624617	25.152551643203395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	624.0
1	465.5
2	307.0
3	307.0
4	653.0
5	999.0
6	999.0
7	1168.5
8	1338.0
9	1280.0
10	1222.0
11	1222.0
12	1617.5
13	2013.0
14	2688.5
15	3364.0
16	3364.0
17	4869.5
18	6375.0
19	6375.0
20	7791.0
21	9207.0
22	10075.5
23	10944.0
24	10944.0
25	13252.0
26	15560.0
27	15560.0
28	19707.0
29	23854.0
30	29089.5
31	34325.0
32	34325.0
33	43550.0
34	52775.0
35	52775.0
36	58667.0
37	64559.0
38	73420.0
39	82281.0
40	82281.0
41	86916.0
42	91551.0
43	103743.0
44	115935.0
45	115935.0
46	118172.0
47	120409.0
48	120409.0
49	129538.5
50	138668.0
51	140528.0
52	142388.0
53	142388.0
54	134966.0
55	127544.0
56	127544.0
57	124560.0
58	121576.0
59	108579.5
60	95583.0
61	95583.0
62	91235.5
63	86888.0
64	71532.0
65	56176.0
66	56176.0
67	46995.5
68	37815.0
69	37815.0
70	30601.0
71	23387.0
72	18279.0
73	13171.0
74	13171.0
75	10011.0
76	6851.0
77	6851.0
78	6825.0
79	6799.0
80	5301.5
81	3804.0
82	3804.0
83	3982.0
84	4160.0
85	4160.0
86	2757.0
87	1354.0
88	1191.0
89	1028.0
90	1028.0
91	710.5
92	393.0
93	378.5
94	364.0
95	364.0
96	318.0
97	272.0
98	272.0
99	516.5
100	761.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11821131217875196
2	0.0541608257932968
3	0.01287647083811223
4	0.0035841722951446414
5	1.3274712204239413E-4
6	3.3186780510598526E-4
7	0.0
8	3.9824136612718236E-4
9	1.9912068306359118E-4
10	4.6461492714837936E-4
11	0.0025885688798266853
12	0.002522195318805488
13	0.014004821375472579
14	0.008894057176840407
15	0.02323074635741897
16	0.010885264007476319
17	0.01938107981818954
18	0.005243511320674568
19	0.007367465273352874
20	0.005376258442716962
21	0.006438235419056115
22	0.007765706639480056
23	0.010287901958285543
24	0.014469436302620958
25	0.016792510938362856
26	0.01944745337921074
27	0.00882768361581921
28	0.005442632003738159
29	0.011748120300751879
30	0.0035841722951446414
31	0.006239114735992524
32	0.0067701032241621
33	0.008097574444586041
34	0.012013614544836668
35	0.01626152245019328
36	0.015000424790790536
37	0.009425045665009983
38	0.00564175268680175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1506624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.75418330585672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.67766889679612	44.72194054770247
2	11.459073402954964	12.548644112413267
3	3.167042693809903	5.202265085830251
4	1.2339622165609578	2.7025837359231977
5	0.6167624193833007	1.688516128353846
6	0.39564367805901096	1.299788788334787
7	0.2628501377544766	1.0074501237204807
8	0.18442294425661057	0.80783421565058
9	0.13554361477380616	0.6679421936337071
>10	0.6901137988037842	6.875634734613113
>50	0.07285236854417067	2.865894639517087
>100	0.08491031518156626	10.037691005724573
>500	0.014029643369154767	5.189501335026208
>1k	0.005123869752213045	4.384313353556426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3885	0.2578612845673506	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2400	0.15929654645087293	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2340	0.15531413278960113	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2232	0.14814578819931185	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2135	0.1417075527802557	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2132	0.14150843209719213	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2029	0.13467195531200882	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2017	0.13387547257975446	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1985	0.13175151862707615	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1944	0.1290302026252071	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1872	0.1242513062316809	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1856	0.12318932925534175	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1845	0.12245922008410857	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1578	0.10473747929144897	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1566	0.1039409965591946	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1522	0.10102055987426192	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.637356102119706E-5
2	0.0	0.0	0.0	0.0	6.637356102119706E-5
3	0.0	0.0	0.0	0.0	6.637356102119706E-5
4	0.0	0.0	0.0	0.0	6.637356102119706E-5
5	0.0	0.0	0.0	0.0	6.637356102119706E-5
6	0.0	0.0	0.0	0.0	6.637356102119706E-5
7	0.0	0.0	0.0	0.0	6.637356102119706E-5
8	0.0	0.0	0.0	0.0	6.637356102119706E-5
9	0.0	0.0	0.0	6.637356102119706E-5	6.637356102119706E-5
10	0.0	0.0	0.0	6.637356102119706E-5	6.637356102119706E-5
11	0.0	0.0	0.0	6.637356102119706E-5	6.637356102119706E-5
12	0.0	0.0	0.0	6.637356102119706E-5	2.6549424408478826E-4
13	0.0	0.0	0.0	6.637356102119706E-5	3.318678051059853E-4
14	0.0	0.0	0.0	6.637356102119706E-5	3.318678051059853E-4
15	0.0	0.0	0.0	6.637356102119706E-5	5.309884881695765E-4
16	0.0	0.0	0.0	1.3274712204239413E-4	5.973620491907736E-4
17	0.0	0.0	0.0	1.9912068306359118E-4	6.637356102119706E-4
18	6.637356102119706E-5	0.0	0.0	1.9912068306359118E-4	6.637356102119706E-4
19	6.637356102119706E-5	0.0	0.0	1.9912068306359118E-4	6.637356102119706E-4
20	6.637356102119706E-5	0.0	0.0	5.309884881695765E-4	8.628562932755618E-4
21	6.637356102119706E-5	0.0	0.0	6.637356102119706E-4	9.292298542967588E-4
22	6.637356102119706E-5	0.0	0.0	8.628562932755618E-4	9.956034153179559E-4
23	6.637356102119706E-5	0.0	0.0	0.0017920861475723207	9.956034153179559E-4
24	6.637356102119706E-5	0.0	0.0	0.003185930929017459	9.956034153179559E-4
25	6.637356102119706E-5	0.0	0.0	0.0037169194171870353	9.956034153179559E-4
26	6.637356102119706E-5	0.0	0.0	0.004380655027399006	9.956034153179559E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	455	0.0	17.230324	28
CGAGCCG	560	0.0	16.285833	15
ACGTAAC	60	0.0024255342	16.016598	1
CTAGCGG	490	0.0	15.999585	29
AGGCCCG	635	0.0	15.370191	10
TAGGACG	295	0.0	15.183527	4
TACCGTC	330	0.0	15.027918	7
GATATAC	320	0.0	15.015559	1
TAGCGGC	515	0.0	14.912234	30
CGCAAGA	540	0.0	14.820334	2
GCGCAAG	530	0.0	14.807798	1
CGTAACA	65	0.0041520833	14.774734	2
CAAGACG	565	0.0	14.722837	4
AAGACGG	555	0.0	14.699394	5
TATACAC	455	0.0	14.41673	3
ACCGTCG	370	0.0	14.268006	8
TGGATAC	655	0.0	14.167573	24
AGAGCGA	590	0.0	14.101797	15
ATACCGT	380	0.0	13.89161	6
GAGCCGC	680	0.0	13.882453	16
>>END_MODULE