FastQCFastQC Report
Thu 2 Feb 2017
SRR4062942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506624
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45950.3049865128924005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29470.19560288432946774No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24390.16188511533069963No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23510.15604424196083427No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22150.1470174376619515No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21600.14336689180578566No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21520.1428359033176161No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20980.13925173102247143No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19710.1308222887727794No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA19700.1307559152117582No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18820.12491504184189287No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18600.12345482349942653No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG18580.12332207637738414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17690.11741482944649759No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17660.11721570876343401No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16890.11210494456480183No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16560.10991461705110234No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15690.10414011724225818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT3900.018.05096429
CATCGTT4000.017.5996928
GATATAC2150.017.1176821
GTACTAA753.2324955E-517.0680661
ACGAACG1900.016.84180815
CTAGCGG4900.016.6527729
CGTAAAC1252.8558134E-916.63970832
CGGCATC4250.016.56496425
TCTAGCG4850.016.16466328
ATAATAC1601.8189894E-1116.000253
ACTATAC1304.803951E-916.0002483
TCGTTTA4400.015.99971930
CGTTTAT4400.015.99971931
CGAACGA2000.015.99971816
GCATCGT4450.015.81994527
GGCATCG4400.015.63608826
ACCGTCG4850.015.5053968
CTATACT1551.891749E-1015.4841124
ATACCGT4900.015.34717756
CGTCGTA4900.015.34666910
Produced by FastQC (version 0.11.5)