##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062942_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1506624
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19857641986322	32.0	32.0	32.0	32.0	32.0
2	31.27928335138694	32.0	32.0	32.0	32.0	32.0
3	31.364687539824136	32.0	32.0	32.0	32.0	32.0
4	31.46271133341829	32.0	32.0	32.0	32.0	32.0
5	31.385595875281425	32.0	32.0	32.0	32.0	32.0
6	34.9017976615267	36.0	36.0	36.0	36.0	36.0
7	34.917182389235805	36.0	36.0	36.0	36.0	36.0
8	34.86373043307421	36.0	36.0	36.0	32.0	36.0
9	34.970449163162144	36.0	36.0	36.0	36.0	36.0
10	34.81748664563952	36.0	36.0	36.0	32.0	36.0
11	34.9958788655962	36.0	36.0	36.0	36.0	36.0
12	34.88743110424366	36.0	36.0	36.0	32.0	36.0
13	34.93619575952594	36.0	36.0	36.0	36.0	36.0
14	34.87833859011937	36.0	36.0	36.0	32.0	36.0
15	34.85926946603798	36.0	36.0	36.0	32.0	36.0
16	34.8699237500531	36.0	36.0	36.0	32.0	36.0
17	34.83656838069751	36.0	36.0	36.0	32.0	36.0
18	34.82881528503462	36.0	36.0	36.0	32.0	36.0
19	34.822572187884965	36.0	36.0	36.0	32.0	36.0
20	34.810524722824006	36.0	36.0	36.0	32.0	36.0
21	34.79921732296844	36.0	36.0	36.0	32.0	36.0
22	34.78103362219107	36.0	36.0	36.0	32.0	36.0
23	34.72997045049063	36.0	36.0	36.0	32.0	36.0
24	34.704425921796016	36.0	36.0	36.0	32.0	36.0
25	34.68404724735568	36.0	36.0	36.0	32.0	36.0
26	34.611776395437744	36.0	36.0	36.0	32.0	36.0
27	34.60244958264305	36.0	36.0	36.0	32.0	36.0
28	34.563852029438	36.0	36.0	36.0	32.0	36.0
29	34.53153208763434	36.0	36.0	36.0	32.0	36.0
30	34.51650511341914	36.0	36.0	36.0	32.0	36.0
31	34.511195228537446	36.0	36.0	36.0	32.0	36.0
32	34.47770180217493	36.0	36.0	36.0	32.0	36.0
33	34.44767506690455	36.0	36.0	36.0	32.0	36.0
34	34.434663194001956	36.0	36.0	36.0	32.0	36.0
35	34.391889416337456	36.0	36.0	36.0	32.0	36.0
36	34.38141898708636	36.0	36.0	36.0	32.0	36.0
37	34.368117725457715	36.0	36.0	36.0	32.0	36.0
38	33.92757848009855	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	13.0
21	35.0
22	126.0
23	354.0
24	1073.0
25	2511.0
26	5489.0
27	10674.0
28	18546.0
29	28625.0
30	43243.0
31	62211.0
32	88695.0
33	140244.0
34	341172.0
35	763612.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89162192732313	18.619067722355865	12.077963284531604	26.411347065789403
2	15.853988785523129	20.90753897455503	36.55703081857185	26.681441421349984
3	18.01265611061552	24.599236438554012	28.705171296886284	28.682936153944183
4	12.12653206975605	16.221520726499413	36.186780758544614	35.46516644519993
5	14.342084477725667	36.73655369429093	33.02082406867814	15.900537759305253
6	34.05058863431661	36.09018314468849	16.52112041300312	13.338107807991781
7	30.10085522844257	30.65142720602145	20.73124188993207	18.516475675603917
8	28.057573535024343	32.75432349105101	19.361606802093462	19.82649617183118
9	27.560407203927873	14.175672240265452	18.521977449393223	39.74194310641346
10	15.98546667144562	26.4997617160691	30.91996182147157	26.594809791013706
11	37.632165337684135	21.236395159507772	22.167534770046085	18.963904732762003
12	25.271635601421465	23.775300110048097	28.104544362391433	22.848519926139012
13	29.3316633712128	19.180868977665916	25.247889150622488	26.2395785004988
14	23.769434178667005	19.99702646446625	24.916900301601462	31.316639055265284
15	25.26058260056922	27.01038878977104	22.25093984962406	25.478088760035682
16	26.018104052504142	25.843408839896355	23.36594930121915	24.772537806380356
17	24.089288369228154	25.915158659360266	24.921413703750904	25.074139267660676
18	25.24133426787307	24.554434284864705	26.144014697761353	24.06021674950087
19	25.759446285204536	24.74539101992269	25.457778450363193	24.03738424450958
20	26.003501869079475	24.02629985981904	24.856035746145025	25.114162524956456
21	27.355585777710516	24.07214554954431	24.271266629099156	24.301002043646015
22	25.880794332756103	24.15700543533452	24.965502318761892	24.996697913147482
23	24.235308173262005	23.8082595478621	25.683450372356663	26.272981906519227
24	24.91238689945202	24.960839598997495	25.18305828129646	24.943715220254028
25	24.96877119808419	24.33304438671412	25.338671544687386	25.3595128705143
26	24.736961577672997	25.43580880166518	25.674620874219446	24.15260874644238
27	25.51580587844471	24.41865018654302	24.83939246911802	25.226151465894254
28	24.386973790408224	24.65631770103224	25.905136251646066	25.051572256913467
29	24.527884860456226	24.665941867380315	25.676213839683953	25.129959432479502
30	24.4733257932968	24.880394843039802	25.95757136485281	24.688707998810585
31	25.307442334650187	24.62505575379126	24.622467184911432	25.445034726647126
32	24.7905250414171	24.770015611061552	24.61967949534854	25.819779852172804
33	24.39613334182915	24.217787583365194	25.483664139161462	25.902414935644195
34	25.37102820610849	24.383522365235123	25.53742672358863	24.70802270506775
35	26.07697740112994	24.30228112654518	25.444503738158957	24.176237734165923
36	24.58649271483794	25.070024106877366	25.04254545261459	25.30093772567011
37	25.67428900641434	24.91855964062699	24.7848832887303	24.62226806422837
38	24.75632557889476	24.465343307971946	25.5066801803506	25.2716509327827
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	268.0
1	216.5
2	165.0
3	165.0
4	587.5
5	1010.0
6	1010.0
7	1153.5
8	1297.0
9	1264.0
10	1231.0
11	1231.0
12	1589.5
13	1948.0
14	2634.0
15	3320.0
16	3320.0
17	5067.5
18	6815.0
19	6815.0
20	8271.5
21	9728.0
22	10420.5
23	11113.0
24	11113.0
25	13215.5
26	15318.0
27	15318.0
28	19464.0
29	23610.0
30	28779.5
31	33949.0
32	33949.0
33	43818.5
34	53688.0
35	53688.0
36	58885.0
37	64082.0
38	73222.0
39	82362.0
40	82362.0
41	87304.0
42	92246.0
43	104196.5
44	116147.0
45	116147.0
46	118362.5
47	120578.0
48	120578.0
49	130268.0
50	139958.0
51	140873.5
52	141789.0
53	141789.0
54	134852.5
55	127916.0
56	127916.0
57	124970.0
58	122024.0
59	108893.0
60	95762.0
61	95762.0
62	91590.0
63	87418.0
64	71640.5
65	55863.0
66	55863.0
67	46990.0
68	38117.0
69	38117.0
70	30809.5
71	23502.0
72	18339.5
73	13177.0
74	13177.0
75	9801.0
76	6425.0
77	6425.0
78	6655.5
79	6886.0
80	5303.0
81	3720.0
82	3720.0
83	3600.0
84	3480.0
85	3480.0
86	2209.5
87	939.0
88	755.5
89	572.0
90	572.0
91	340.5
92	109.0
93	82.5
94	56.0
95	56.0
96	37.5
97	19.0
98	19.0
99	18.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007964827322543647
2	0.0
3	0.0
4	1.3274712204239413E-4
5	1.3274712204239413E-4
6	6.637356102119706E-5
7	5.973620491907736E-4
8	0.0019248332696147148
9	0.0037832929782082325
10	0.0011947240983815471
11	0.005509005564759356
12	6.637356102119705E-4
13	1.9912068306359118E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.9912068306359118E-4
22	6.637356102119706E-5
23	2.6549424408478826E-4
24	0.0
25	1.3274712204239413E-4
26	0.0
27	6.637356102119706E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.9912068306359118E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1506624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.83140828777514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.33404878544897	42.44160930794101
2	12.34170164691407	13.04058957348048
3	3.3486842950074607	5.3074712164919875
4	1.3251109332165418	2.8002990695743137
5	0.6792804759240804	1.794367208272965
6	0.41605467693198206	1.3188452704219156
7	0.27161334548113064	1.0044800886065497
8	0.19761233372210862	0.8352110308458236
9	0.14837114261925397	0.7054790772457368
>10	0.7473401341818369	7.14031621586886
>50	0.0785194817650503	2.9267093751157294
>100	0.09178634258996818	10.624812408503725
>500	0.013672941842967341	4.976597529178281
>1k	0.0062034643546796265	5.083212628452659
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4595	0.3049865128924005	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2947	0.19560288432946774	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2439	0.16188511533069963	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2351	0.15604424196083427	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2215	0.1470174376619515	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2160	0.14336689180578566	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2152	0.1428359033176161	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2098	0.13925173102247143	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1971	0.1308222887727794	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1970	0.1307559152117582	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1882	0.12491504184189287	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1860	0.12345482349942653	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1858	0.12332207637738414	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1769	0.11741482944649759	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1766	0.11721570876343401	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1689	0.11210494456480183	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1656	0.10991461705110234	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1569	0.10414011724225818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6549424408478826E-4
2	0.0	0.0	0.0	0.0	2.6549424408478826E-4
3	0.0	0.0	0.0	0.0	2.6549424408478826E-4
4	0.0	0.0	0.0	6.637356102119706E-5	2.6549424408478826E-4
5	0.0	0.0	0.0	6.637356102119706E-5	2.6549424408478826E-4
6	0.0	0.0	0.0	6.637356102119706E-5	2.6549424408478826E-4
7	0.0	0.0	0.0	6.637356102119706E-5	2.6549424408478826E-4
8	0.0	0.0	0.0	6.637356102119706E-5	2.6549424408478826E-4
9	0.0	0.0	0.0	6.637356102119706E-5	2.6549424408478826E-4
10	0.0	0.0	0.0	6.637356102119706E-5	3.318678051059853E-4
11	0.0	0.0	0.0	6.637356102119706E-5	3.318678051059853E-4
12	0.0	0.0	0.0	6.637356102119706E-5	5.973620491907736E-4
13	0.0	0.0	0.0	6.637356102119706E-5	6.637356102119706E-4
14	0.0	0.0	0.0	6.637356102119706E-5	6.637356102119706E-4
15	0.0	0.0	0.0	6.637356102119706E-5	7.301091712331677E-4
16	0.0	0.0	0.0	1.3274712204239413E-4	7.301091712331677E-4
17	0.0	0.0	0.0	1.9912068306359118E-4	7.301091712331677E-4
18	0.0	0.0	0.0	1.9912068306359118E-4	7.301091712331677E-4
19	0.0	0.0	0.0	2.6549424408478826E-4	7.964827322543647E-4
20	0.0	0.0	0.0	5.309884881695765E-4	8.628562932755618E-4
21	0.0	0.0	0.0	5.973620491907736E-4	9.956034153179559E-4
22	0.0	0.0	0.0	7.301091712331677E-4	9.956034153179559E-4
23	0.0	0.0	0.0	0.0015929654645087294	9.956034153179559E-4
24	0.0	0.0	0.0	0.002986810245953868	9.956034153179559E-4
25	0.0	0.0	0.0	0.003451425173102247	9.956034153179559E-4
26	0.0	0.0	0.0	0.004115160783314218	0.001261097659402744
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	390	0.0	18.050964	29
CATCGTT	400	0.0	17.59969	28
GATATAC	215	0.0	17.117682	1
GTACTAA	75	3.2324955E-5	17.068066	1
ACGAACG	190	0.0	16.841808	15
CTAGCGG	490	0.0	16.65277	29
CGTAAAC	125	2.8558134E-9	16.639708	32
CGGCATC	425	0.0	16.564964	25
TCTAGCG	485	0.0	16.164663	28
ATAATAC	160	1.8189894E-11	16.00025	3
ACTATAC	130	4.803951E-9	16.000248	3
TCGTTTA	440	0.0	15.999719	30
CGTTTAT	440	0.0	15.999719	31
CGAACGA	200	0.0	15.999718	16
GCATCGT	445	0.0	15.819945	27
GGCATCG	440	0.0	15.636088	26
ACCGTCG	485	0.0	15.505396	8
CTATACT	155	1.891749E-10	15.484112	4
ATACCGT	490	0.0	15.3471775	6
CGTCGTA	490	0.0	15.346669	10
>>END_MODULE