FastQCFastQC Report
Thu 2 Feb 2017
SRR4062941_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062941_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003689
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49270.24589644400902533No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT33240.1658940085013193No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32200.1607035822425536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28460.1420380108889154No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG26790.13370338410801277No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26410.13180688220577147No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26230.13090853919944662No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA26110.13030964386189672No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25840.1289621293524095No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG25110.12531884938231433No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23690.1182319212213073No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22680.11319121879692906No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA22320.1113945327842794No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21910.10934830704765061No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21800.10879931965489655No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21640.10800079253816336No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG20880.10420778873368074No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20770.10365880134092667No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC20230.10096377232195217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6200.019.35444628
CTAGCGG6350.018.64575629
TAGCGGC6600.017.9394830
CGCACGA957.7010554E-716.84176428
CACGATT908.586794E-616.00007430
GTATCAA42150.015.9542861
CGCCGGT8150.015.7055957
CAAGACG7550.015.6778884
CTATTCC7200.015.5513584
CGGTCCA8150.015.50927510
CGCAAGA7450.015.46624852
GTATTAG4250.015.4461621
AAGACGG7700.015.3724755
GTCCTAT7450.015.2590591
CCTATTC7450.015.2445883
CGCTTCG5050.015.20799132
GGTCCAA8750.015.17721511
GCGCAAG7350.015.0309851
AGCGGCG7900.014.78487931
GGGTATA1308.2480256E-814.779631
Produced by FastQC (version 0.11.5)