##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062941_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2003689
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24486434771065	32.0	32.0	32.0	32.0	32.0
2	30.85754525777204	32.0	32.0	32.0	32.0	32.0
3	30.898113928858223	32.0	32.0	32.0	32.0	32.0
4	30.968286495558942	32.0	32.0	32.0	32.0	32.0
5	30.874998565146587	32.0	32.0	32.0	32.0	32.0
6	34.52803304305209	36.0	36.0	36.0	32.0	36.0
7	34.456402166204434	36.0	36.0	36.0	32.0	36.0
8	34.4455581679592	36.0	36.0	36.0	32.0	36.0
9	34.57917620948161	36.0	36.0	36.0	32.0	36.0
10	34.296887890286364	36.0	36.0	36.0	32.0	36.0
11	34.557801634884456	36.0	36.0	36.0	32.0	36.0
12	34.37348959843568	36.0	36.0	36.0	32.0	36.0
13	34.458617080794475	36.0	36.0	36.0	32.0	36.0
14	34.360097300529176	36.0	36.0	36.0	32.0	36.0
15	34.2997031974523	36.0	36.0	36.0	32.0	36.0
16	34.315979176409115	36.0	36.0	36.0	32.0	36.0
17	34.23350330315733	36.0	36.0	36.0	32.0	36.0
18	34.25704937243255	36.0	36.0	36.0	32.0	36.0
19	34.251870924080535	36.0	36.0	36.0	32.0	36.0
20	34.22844213847558	36.0	36.0	36.0	32.0	36.0
21	34.21453628781712	36.0	36.0	36.0	32.0	36.0
22	34.19476176193012	36.0	36.0	36.0	32.0	36.0
23	34.141946180270494	36.0	36.0	36.0	32.0	36.0
24	34.1242593037143	36.0	36.0	36.0	32.0	36.0
25	34.10714686760271	36.0	36.0	36.0	32.0	36.0
26	34.049436314717504	36.0	36.0	36.0	32.0	36.0
27	34.04835630679212	36.0	36.0	36.0	32.0	36.0
28	34.037326151912794	36.0	36.0	36.0	32.0	36.0
29	34.00843045003491	36.0	36.0	36.0	32.0	36.0
30	33.98747909480962	36.0	36.0	36.0	32.0	36.0
31	34.00476171701297	36.0	36.0	36.0	32.0	36.0
32	33.96082026701749	36.0	36.0	36.0	32.0	36.0
33	33.919515952825016	36.0	36.0	36.0	32.0	36.0
34	33.92748974516504	36.0	36.0	36.0	32.0	36.0
35	33.88843528112397	36.0	36.0	36.0	32.0	36.0
36	33.846030496748746	36.0	36.0	36.0	32.0	36.0
37	33.853334524469616	36.0	36.0	36.0	32.0	36.0
38	33.261105391106106	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	4.0
9	15.0
10	17.0
11	20.0
12	10.0
13	10.0
14	201.0
15	480.0
16	672.0
17	720.0
18	879.0
19	1239.0
20	1808.0
21	2478.0
22	3886.0
23	5943.0
24	8973.0
25	13647.0
26	19854.0
27	28506.0
28	39465.0
29	53655.0
30	71721.0
31	96776.0
32	130611.0
33	195855.0
34	432412.0
35	893828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.891547548319814	17.001790335386833	11.499569530020722	25.60709258627263
2	17.220156846491676	18.975579186006154	37.05691864805415	26.74734531944802
3	19.60266963359174	22.79995068435441	27.672915478074756	29.924464203979095
4	12.867160439604316	14.7523482496681	35.29930825206377	37.08118305866382
5	15.394800474925423	35.77292416015907	32.806586670646006	16.0256886942695
6	35.335626440470215	34.58850338825544	16.39448496421096	13.681385207063382
7	31.670898862497555	29.747046446352925	19.774036676368777	18.808018014780746
8	28.940456149999775	31.943426180688263	18.907531780226712	20.20858588908525
9	27.525647756831916	14.494101864365838	18.36891445956548	39.611335919236765
10	16.532530144534057	25.529226223748303	30.287071787910246	27.651171843807393
11	38.19341437267049	21.0681980160069	21.554414075211266	19.18397353611135
12	25.888076085942057	23.123941607952368	27.363798387825288	23.62418391828028
13	29.456215898376097	18.872239861794053	25.008198615644407	26.663345624185443
14	24.118785076396918	19.568960870164172	24.299071018286146	32.013183035152764
15	25.595873390195855	26.577805245023693	21.930626789017936	25.895694575762505
16	26.58134338005799	25.47594411409581	22.945145821759485	24.997566684086713
17	24.637800530838184	25.57298226329554	24.340283549657133	25.448933656209142
18	25.673401345604823	24.264858551777834	25.56329733075126	24.498442771866078
19	25.987739541625366	24.567435016675702	25.024382160543922	24.42044328115501
20	26.389403251615377	23.635851819612906	24.44621019206831	25.528534736703406
21	27.625141061481095	23.593116049989042	24.069119002734634	24.712623885795225
22	26.486447096333997	23.650636704718046	24.644288374604884	25.21862782434307
23	24.69176102757305	23.5444561906844	25.409562770342376	26.354220011400177
24	25.155025739039633	24.61948000800648	24.86182081555294	25.363673437400948
25	25.31441227429579	24.06325233697109	24.8427002692003	25.779635119532816
26	24.965057585099757	25.19452941552322	25.305047282079133	24.535365717297893
27	25.89084102820065	24.1161467431994	24.44077863738458	25.552233591215373
28	24.836827701932112	24.268688138146306	25.432168887633345	25.462315272288233
29	24.742972484903785	24.62123443085588	25.370030776418258	25.265762307822083
30	24.669011423828582	24.738585529484396	25.582857818776922	25.009545227910095
31	25.565714068958773	24.66736941086279	24.122742658868603	25.644173861309838
32	25.265954526442723	24.39095240519521	24.004585887564897	26.338507180797173
33	24.67307147106857	24.049611279483464	25.011729496839525	26.26558775260844
34	25.754161891489336	24.275537382151285	24.959968374017176	25.010332352342203
35	26.28957012831559	23.989663272671365	25.13584799799535	24.584918601017698
36	24.6738635076039	24.967367632385084	24.596194729005347	25.762574131005668
37	26.09796989557737	24.661628465884334	24.35496405527746	24.885437583260835
38	24.89194277409102	24.32520383635594	25.06119083554938	25.721662554003654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	126.0
1	187.0
2	248.0
3	248.0
4	518.5
5	789.0
6	789.0
7	1018.5
8	1248.0
9	1264.0
10	1280.0
11	1280.0
12	1669.5
13	2059.0
14	2809.0
15	3559.0
16	3559.0
17	5270.0
18	6981.0
19	6981.0
20	8719.0
21	10457.0
22	11014.5
23	11572.0
24	11572.0
25	13735.5
26	15899.0
27	15899.0
28	20603.0
29	25307.0
30	31596.0
31	37885.0
32	37885.0
33	49509.0
34	61133.0
35	61133.0
36	69496.0
37	77859.0
38	90097.5
39	102336.0
40	102336.0
41	109788.0
42	117240.0
43	135289.0
44	153338.0
45	153338.0
46	157378.5
47	161419.0
48	161419.0
49	174143.0
50	186867.0
51	190819.0
52	194771.0
53	194771.0
54	187171.0
55	179571.0
56	179571.0
57	174455.0
58	169339.0
59	151786.5
60	134234.0
61	134234.0
62	127359.5
63	120485.0
64	101409.0
65	82333.0
66	82333.0
67	69105.5
68	55878.0
69	55878.0
70	44913.5
71	33949.0
72	26655.0
73	19361.0
74	19361.0
75	14662.5
76	9964.0
77	9964.0
78	9658.0
79	9352.0
80	7368.5
81	5385.0
82	5385.0
83	5498.5
84	5612.0
85	5612.0
86	3808.0
87	2004.0
88	1683.0
89	1362.0
90	1362.0
91	1000.0
92	638.0
93	551.0
94	464.0
95	464.0
96	429.5
97	395.0
98	395.0
99	692.5
100	990.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11918017217242796
2	0.05459929160663157
3	0.01337532920528086
4	0.003443648190911863
5	2.994476687749446E-4
6	1.497238343874723E-4
7	4.9907944795824104E-5
8	2.994476687749446E-4
9	2.4953972397912053E-4
10	4.491715031624169E-4
11	0.0026451210741786774
12	0.002994476687749446
13	0.015072199328338878
14	0.010231128683143942
15	0.024704432673932932
16	0.012476986198956026
17	0.022059311599754255
18	0.006438124878661309
19	0.008733890339269218
20	0.006537940768252958
21	0.0064880328234571336
22	0.008484350615290098
23	0.010530576351918886
24	0.01557127877629712
25	0.017367964788946786
26	0.019613822304758874
27	0.009432601566410756
28	0.006587848713048781
29	0.010330944572735588
30	0.0034935561357076874
31	0.005739413651519772
32	0.007536099664169439
33	0.009632233345594052
34	0.01362486892925998
35	0.017318056844150966
36	0.015471462886705473
37	0.009781957179981524
38	0.0060388613202947165
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2003689.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.53241241869318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27915939297452	41.647254974390506
2	12.574812472389127	13.21170469774545
3	3.800167520550236	5.988959023490031
4	1.5308118131949444	3.216689500246573
5	0.7698116178443085	2.0220030696649323
6	0.44494905844597094	1.4024548466155777
7	0.2981893969672463	1.096522586625536
8	0.19693843319950022	0.8276520795141922
9	0.16272457842849813	0.7693483198197463
>10	0.7779868929826322	7.309270550380143
>50	0.06890498323564666	2.5502952706610746
>100	0.07414365959448865	8.525352077227518
>500	0.013948331732923059	5.006367455782515
>1k	0.007451848460054785	6.426125547836204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4927	0.24589644400902533	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3324	0.1658940085013193	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3220	0.1607035822425536	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2846	0.1420380108889154	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2679	0.13370338410801277	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2641	0.13180688220577147	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2623	0.13090853919944662	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2611	0.13030964386189672	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2584	0.1289621293524095	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2511	0.12531884938231433	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2369	0.1182319212213073	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2268	0.11319121879692906	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2232	0.1113945327842794	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2191	0.10934830704765061	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2180	0.10879931965489655	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2164	0.10800079253816336	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2088	0.10420778873368074	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2077	0.10365880134092667	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2023	0.10096377232195217	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.9907944795824104E-5	0.0	0.0	0.0
6	0.0	4.9907944795824104E-5	0.0	0.0	4.9907944795824104E-5
7	0.0	4.9907944795824104E-5	0.0	0.0	4.9907944795824104E-5
8	0.0	4.9907944795824104E-5	0.0	0.0	4.9907944795824104E-5
9	0.0	4.9907944795824104E-5	0.0	0.0	4.9907944795824104E-5
10	0.0	4.9907944795824104E-5	0.0	0.0	9.981588959164821E-5
11	0.0	4.9907944795824104E-5	0.0	0.0	9.981588959164821E-5
12	0.0	4.9907944795824104E-5	0.0	0.0	3.493556135707687E-4
13	0.0	4.9907944795824104E-5	0.0	0.0	3.9926355836659283E-4
14	0.0	4.9907944795824104E-5	0.0	0.0	3.9926355836659283E-4
15	0.0	4.9907944795824104E-5	0.0	0.0	4.491715031624169E-4
16	0.0	4.9907944795824104E-5	0.0	4.9907944795824104E-5	4.491715031624169E-4
17	0.0	4.9907944795824104E-5	0.0	4.9907944795824104E-5	4.491715031624169E-4
18	0.0	4.9907944795824104E-5	0.0	4.9907944795824104E-5	4.990794479582411E-4
19	0.0	4.9907944795824104E-5	0.0	1.497238343874723E-4	4.990794479582411E-4
20	0.0	9.981588959164821E-5	0.0	2.4953972397912053E-4	4.990794479582411E-4
21	0.0	9.981588959164821E-5	0.0	2.994476687749446E-4	5.988953375498892E-4
22	0.0	9.981588959164821E-5	0.0	4.491715031624169E-4	5.988953375498892E-4
23	0.0	9.981588959164821E-5	0.0	8.983430063248338E-4	5.988953375498892E-4
24	0.0	9.981588959164821E-5	0.0	0.001447330399078899	5.988953375498892E-4
25	0.0	9.981588959164821E-5	0.0	0.0020462257366287884	5.988953375498892E-4
26	0.0	9.981588959164821E-5	0.0	0.0026451210741786774	6.987112271415374E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	620	0.0	19.354446	28
CTAGCGG	635	0.0	18.645756	29
TAGCGGC	660	0.0	17.93948	30
CGCACGA	95	7.7010554E-7	16.841764	28
CACGATT	90	8.586794E-6	16.000074	30
GTATCAA	4215	0.0	15.954286	1
CGCCGGT	815	0.0	15.705595	7
CAAGACG	755	0.0	15.677888	4
CTATTCC	720	0.0	15.551358	4
CGGTCCA	815	0.0	15.509275	10
CGCAAGA	745	0.0	15.4662485	2
GTATTAG	425	0.0	15.446162	1
AAGACGG	770	0.0	15.372475	5
GTCCTAT	745	0.0	15.259059	1
CCTATTC	745	0.0	15.244588	3
CGCTTCG	505	0.0	15.207991	32
GGTCCAA	875	0.0	15.177215	11
GCGCAAG	735	0.0	15.030985	1
AGCGGCG	790	0.0	14.784879	31
GGGTATA	130	8.2480256E-8	14.77963	1
>>END_MODULE