FastQCFastQC Report
Thu 2 Feb 2017
SRR4062941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003689
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54020.2696027177870418No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC35450.17692366430119644No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT34490.17213250160079732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33890.16913802491304789No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28090.1401914169314699No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC27490.13719694024372048No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27150.13550007012066242No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG27050.1350009906727042No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG25380.12666636389180155No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC25160.12556838910629345No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24360.12157575352262752No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24310.12132621379864839No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA22750.11354057441049982No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21880.10919858321326315No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21480.10720226542143019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21340.10650355419428864No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21210.10585475091194294No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21010.10485659201602644No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG20690.10325953778256007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5950.018.28507228
TACCGTC5500.017.7474887
CTAGCGG6250.017.6633829
ACCGTCG5600.017.1448238
CGTCGTA5850.016.68400810
CCTATAC1354.4383341E-1016.5936663
TAGCGGC6600.016.48427230
ATACCGT6050.016.3985756
GTATCAA39750.016.3427891
GTACCGT1001.3139888E-616.0018376
TCCCCGC4700.015.6601971
GTATTAG4600.015.6527961
CATCGTT5550.015.5670228
ATCGTTT5500.015.41764129
AAGACGG7800.015.3863795
CCGTCGT6250.015.360619
CAAGACG7850.015.2879954
AGCGGCG7300.015.12275731
CGCAAGA7200.014.88948252
CGCCCCT4850.014.8470635
Produced by FastQC (version 0.11.5)