##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062941_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2003689
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20747231731072	32.0	32.0	32.0	32.0	32.0
2	31.276528942365807	32.0	32.0	32.0	32.0	32.0
3	31.35883712492308	32.0	32.0	32.0	32.0	32.0
4	31.461870080636267	32.0	32.0	32.0	32.0	32.0
5	31.373541003618826	32.0	32.0	32.0	32.0	32.0
6	34.90385783422477	36.0	36.0	36.0	36.0	36.0
7	34.92683295661153	36.0	36.0	36.0	36.0	36.0
8	34.85918523283803	36.0	36.0	36.0	32.0	36.0
9	34.9660236693419	36.0	36.0	36.0	32.0	36.0
10	34.80775309940814	36.0	36.0	36.0	32.0	36.0
11	34.99567198302731	36.0	36.0	36.0	36.0	36.0
12	34.88451700837805	36.0	36.0	36.0	32.0	36.0
13	34.94550601415689	36.0	36.0	36.0	36.0	36.0
14	34.88958316385427	36.0	36.0	36.0	32.0	36.0
15	34.85413404974525	36.0	36.0	36.0	32.0	36.0
16	34.87055326450362	36.0	36.0	36.0	32.0	36.0
17	34.82696366551895	36.0	36.0	36.0	32.0	36.0
18	34.829064290915404	36.0	36.0	36.0	32.0	36.0
19	34.82263115683123	36.0	36.0	36.0	32.0	36.0
20	34.81305831393994	36.0	36.0	36.0	32.0	36.0
21	34.80635417971551	36.0	36.0	36.0	32.0	36.0
22	34.79064765040882	36.0	36.0	36.0	32.0	36.0
23	34.73210014128939	36.0	36.0	36.0	32.0	36.0
24	34.70155647907435	36.0	36.0	36.0	32.0	36.0
25	34.67930801636382	36.0	36.0	36.0	32.0	36.0
26	34.615218229974815	36.0	36.0	36.0	32.0	36.0
27	34.604366246458405	36.0	36.0	36.0	32.0	36.0
28	34.57113454233666	36.0	36.0	36.0	32.0	36.0
29	34.53770470367407	36.0	36.0	36.0	32.0	36.0
30	34.51625376992138	36.0	36.0	36.0	32.0	36.0
31	34.509559617285916	36.0	36.0	36.0	32.0	36.0
32	34.482933229657895	36.0	36.0	36.0	32.0	36.0
33	34.452805799702446	36.0	36.0	36.0	32.0	36.0
34	34.44254772072912	36.0	36.0	36.0	32.0	36.0
35	34.41517920196198	36.0	36.0	36.0	32.0	36.0
36	34.39811667379519	36.0	36.0	36.0	32.0	36.0
37	34.38004700330241	36.0	36.0	36.0	32.0	36.0
38	33.93884779524168	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	13.0
21	39.0
22	173.0
23	473.0
24	1340.0
25	3242.0
26	7049.0
27	13767.0
28	24196.0
29	37645.0
30	57022.0
31	82133.0
32	118523.0
33	189216.0
34	460034.0
35	1008818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.09044943960429	18.314195942590324	12.190397280751885	26.404957337053496
2	16.221828836710685	20.01243705984312	36.2690018261317	27.496732277314496
3	18.470630921265727	23.51981769625925	28.044022799945502	29.965528582529526
4	12.344255708856432	15.66548634141594	35.267619411234804	36.722638538492824
5	14.811787064652782	36.26063538834389	32.51334539109882	16.41423215590451
6	35.28417127234506	34.996152091695	16.054112271084392	13.665564364875534
7	31.427925475237352	30.02441004889496	19.713797042745785	18.833867433121906
8	28.821923797345917	32.2042831239884	18.66077136957853	20.313021709087153
9	27.39403528122918	14.330248076601542	18.18937270882879	40.08634393334049
10	16.40948858844021	25.98332060668673	30.158109868391502	27.449080936481558
11	38.28864530438801	21.03899292970532	21.435729993202216	19.23663177270445
12	25.721971572306956	23.30951050067875	27.25584923740318	23.712668689611117
13	29.409099030486814	18.883048541537946	24.966386948853263	26.741465479121977
14	23.96908901531126	19.597402590921046	24.22127386036456	32.21223453340314
15	25.517732542325682	26.75864368172905	21.759963746868902	25.96366002907637
16	26.60699999550828	25.519604608903485	22.764882938303238	25.10851245728499
17	24.677382567853595	25.73787648682006	24.15574472884764	25.428996216478705
18	25.801159760821164	24.210094480730294	25.477357014985856	24.511388743462685
19	25.991059490769274	24.53843884954202	24.9509779212243	24.519523738464404
20	26.359080675693686	23.63934722404525	24.438123880502413	25.563448219758655
21	27.55729812091409	23.748020524163703	24.030000693721128	24.66468066120108
22	26.29915435936134	23.76003649270745	24.625141239554264	25.315667908376955
23	24.511138227815252	23.466612765978685	25.467925347547144	26.55432365865892
24	25.126504163071218	24.571877172555222	24.905661507349695	25.39595715702387
25	25.274269521272295	24.099384833751397	24.906796773546354	25.719548871429954
26	25.04098694057505	25.17369229824818	25.181278313429196	24.604042447747577
27	25.903334251639976	24.10100774172426	24.427705311405767	25.567952695229994
28	24.838984493102473	24.18913314391605	25.52222425735731	25.449658105624177
29	24.81368116509099	24.4800964620757	25.40983156567711	25.2963908071562
30	24.785083912722982	24.582856920410304	25.64120479775055	24.990854369116164
31	25.537545996409623	24.512935889751354	24.26818732847263	25.68133078536639
32	25.29065139350468	24.37563913361804	24.097252617546935	26.236456855330342
33	24.6976451934407	23.96165273153668	25.05114316642952	26.2895589085931
34	25.755993070780946	24.169718953390472	25.05533543379237	25.018952542036217
35	26.278229805124447	23.946081452760385	25.156898101451873	24.618790640663295
36	24.8159769305516	24.770860148456173	24.681674651106032	25.731488269886192
37	26.197378934555214	24.48443845327294	24.428691278936	24.889491333235846
38	24.981384327300457	24.12142010133314	25.108100662378575	25.789094908987824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	63.0
2	85.0
3	85.0
4	366.5
5	648.0
6	648.0
7	863.5
8	1079.0
9	1154.0
10	1229.0
11	1229.0
12	1647.5
13	2066.0
14	2915.0
15	3764.0
16	3764.0
17	5530.5
18	7297.0
19	7297.0
20	8950.0
21	10603.0
22	11341.5
23	12080.0
24	12080.0
25	13933.0
26	15786.0
27	15786.0
28	20721.5
29	25657.0
30	31538.5
31	37420.0
32	37420.0
33	49873.5
34	62327.0
35	62327.0
36	70144.0
37	77961.0
38	90077.5
39	102194.0
40	102194.0
41	110403.0
42	118612.0
43	136203.0
44	153794.0
45	153794.0
46	157170.5
47	160547.0
48	160547.0
49	173708.0
50	186869.0
51	190646.5
52	194424.0
53	194424.0
54	187010.0
55	179596.0
56	179596.0
57	174329.0
58	169062.0
59	152045.0
60	135028.0
61	135028.0
62	128370.0
63	121712.0
64	101754.0
65	81796.0
66	81796.0
67	69268.0
68	56740.0
69	56740.0
70	45355.0
71	33970.0
72	26849.5
73	19729.0
74	19729.0
75	14596.0
76	9463.0
77	9463.0
78	9476.5
79	9490.0
80	7409.5
81	5329.0
82	5329.0
83	5068.0
84	4807.0
85	4807.0
86	3055.5
87	1304.0
88	1086.5
89	869.0
90	869.0
91	522.5
92	176.0
93	124.5
94	73.0
95	73.0
96	52.0
97	31.0
98	31.0
99	31.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009682141290389877
2	0.0
3	0.0
4	1.9963177918329642E-4
5	4.9907944795824104E-5
6	1.497238343874723E-4
7	2.994476687749446E-4
8	0.002894660798157798
9	0.0036931879148909834
10	9.48250951120658E-4
11	0.0047412547556032894
12	4.491715031624169E-4
13	1.497238343874723E-4
14	0.0
15	0.0
16	9.981588959164821E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	9.981588959164821E-5
22	4.9907944795824104E-5
23	1.9963177918329642E-4
24	0.0
25	1.497238343874723E-4
26	9.981588959164821E-5
27	4.9907944795824104E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.9907944795824104E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2003689.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.33205537973484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.50348401142043	39.00909649385207
2	13.604996209949869	13.695348453605588
3	4.099927062404264	6.19073267873415
4	1.6951188222260005	3.4127525774203984
5	0.8614225855740679	2.1678584641234186
6	0.4867531821617941	1.4699572872497748
7	0.3285680596641576	1.157625404251988
8	0.2254659267006689	0.9078530807153042
9	0.1797620141083886	0.8143012484348481
>10	0.8409690790595427	7.640170946882861
>50	0.06985266148800999	2.4813820331601026
>100	0.08060844431711049	8.897212248351668
>500	0.01498177982192182	5.231744718861448
>1k	0.007990282571691636	6.652400864714034
>5k	9.987853214614545E-5	0.2715634996424512
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5402	0.2696027177870418	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3545	0.17692366430119644	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3449	0.17213250160079732	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3389	0.16913802491304789	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2809	0.1401914169314699	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2749	0.13719694024372048	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2715	0.13550007012066242	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2705	0.1350009906727042	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2538	0.12666636389180155	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2516	0.12556838910629345	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2436	0.12157575352262752	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2431	0.12132621379864839	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2275	0.11354057441049982	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2188	0.10919858321326315	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2148	0.10720226542143019	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2134	0.10650355419428864	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2121	0.10585475091194294	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2101	0.10485659201602644	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2069	0.10325953778256007	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	0.0
6	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	9.981588959164821E-5
7	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	9.981588959164821E-5
8	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	9.981588959164821E-5
9	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	9.981588959164821E-5
10	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	9.981588959164821E-5
11	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	9.981588959164821E-5
12	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	3.493556135707687E-4
13	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	3.493556135707687E-4
14	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	3.493556135707687E-4
15	4.9907944795824104E-5	4.9907944795824104E-5	0.0	0.0	4.491715031624169E-4
16	4.9907944795824104E-5	4.9907944795824104E-5	0.0	4.9907944795824104E-5	4.990794479582411E-4
17	4.9907944795824104E-5	4.9907944795824104E-5	0.0	1.497238343874723E-4	4.990794479582411E-4
18	4.9907944795824104E-5	4.9907944795824104E-5	0.0	1.9963177918329642E-4	5.489873927540651E-4
19	4.9907944795824104E-5	4.9907944795824104E-5	0.0	2.4953972397912053E-4	5.489873927540651E-4
20	4.9907944795824104E-5	9.981588959164821E-5	0.0	4.491715031624169E-4	5.489873927540651E-4
21	4.9907944795824104E-5	9.981588959164821E-5	0.0	4.491715031624169E-4	5.988953375498892E-4
22	4.9907944795824104E-5	9.981588959164821E-5	0.0	5.489873927540651E-4	5.988953375498892E-4
23	4.9907944795824104E-5	9.981588959164821E-5	0.0	9.981588959164821E-4	5.988953375498892E-4
24	4.9907944795824104E-5	9.981588959164821E-5	0.0	0.001497238343874723	5.988953375498892E-4
25	4.9907944795824104E-5	9.981588959164821E-5	0.0	0.0018965019022413158	5.988953375498892E-4
26	4.9907944795824104E-5	9.981588959164821E-5	0.0	0.0023955813501995568	5.988953375498892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	595	0.0	18.285072	28
TACCGTC	550	0.0	17.747488	7
CTAGCGG	625	0.0	17.66338	29
ACCGTCG	560	0.0	17.144823	8
CGTCGTA	585	0.0	16.684008	10
CCTATAC	135	4.4383341E-10	16.593666	3
TAGCGGC	660	0.0	16.484272	30
ATACCGT	605	0.0	16.398575	6
GTATCAA	3975	0.0	16.342789	1
GTACCGT	100	1.3139888E-6	16.001837	6
TCCCCGC	470	0.0	15.660197	1
GTATTAG	460	0.0	15.652796	1
CATCGTT	555	0.0	15.56702	28
ATCGTTT	550	0.0	15.417641	29
AAGACGG	780	0.0	15.386379	5
CCGTCGT	625	0.0	15.36061	9
CAAGACG	785	0.0	15.287995	4
AGCGGCG	730	0.0	15.122757	31
CGCAAGA	720	0.0	14.8894825	2
CGCCCCT	485	0.0	14.847063	5
>>END_MODULE