FastQCFastQC Report
Thu 2 Feb 2017
SRR4062940_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062940_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345731
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26820.11433536070418987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21350.021.9021971
GGTATCA7300.021.498391
TAGACCG802.807652E-617.993985
ACGGTAT2000.016.000799
AGCGAAA3800.015.57905117
GAGCGAA3700.015.56800316
GTCCTAC12000.015.4803041
CGACGGT2000.015.1991297
AATAGCG1501.9845174E-914.928345
TAGGACG14100.014.7477834
ACGGACC3800.014.7359978
GTAGGAC14700.014.6914693
TATCAAC31450.014.6559882
ACGACGG2350.014.2933976
TCAACGC32350.014.1414594
GACGGTA2150.014.1387248
TGTAGGA16400.014.052772
CAAGACG4950.013.8952314
GCGCAAG4850.013.86788751
GTATAGA2201.8189894E-1213.8303651
Produced by FastQC (version 0.11.5)