##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062940_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345731
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285736088238593	32.0	32.0	32.0	32.0	32.0
2	30.8791310683109	32.0	32.0	32.0	32.0	32.0
3	30.908855704255945	32.0	32.0	32.0	32.0	32.0
4	30.991568086877823	32.0	32.0	32.0	32.0	32.0
5	30.862075830519355	32.0	32.0	32.0	32.0	32.0
6	34.546558833898686	36.0	36.0	36.0	32.0	36.0
7	34.503101591785246	36.0	36.0	36.0	32.0	36.0
8	34.47305381563359	36.0	36.0	36.0	32.0	36.0
9	34.651617342312484	36.0	36.0	36.0	32.0	36.0
10	34.33403830191953	36.0	36.0	36.0	32.0	36.0
11	34.61862890501937	36.0	36.0	36.0	32.0	36.0
12	34.44437021977371	36.0	36.0	36.0	32.0	36.0
13	34.50872244089369	36.0	36.0	36.0	32.0	36.0
14	34.409730698021214	36.0	36.0	36.0	32.0	36.0
15	34.34727937687655	36.0	36.0	36.0	32.0	36.0
16	34.36881040494413	36.0	36.0	36.0	32.0	36.0
17	34.3194036315332	36.0	36.0	36.0	32.0	36.0
18	34.33902097043523	36.0	36.0	36.0	32.0	36.0
19	34.3201986928595	36.0	36.0	36.0	32.0	36.0
20	34.31150715917554	36.0	36.0	36.0	32.0	36.0
21	34.30853367244582	36.0	36.0	36.0	32.0	36.0
22	34.27699254518101	36.0	36.0	36.0	32.0	36.0
23	34.21329342537572	36.0	36.0	36.0	32.0	36.0
24	34.20780856799011	36.0	36.0	36.0	32.0	36.0
25	34.18197696155271	36.0	36.0	36.0	32.0	36.0
26	34.13616139275987	36.0	36.0	36.0	32.0	36.0
27	34.1382353731097	36.0	36.0	36.0	32.0	36.0
28	34.116104958326424	36.0	36.0	36.0	32.0	36.0
29	34.08760126374252	36.0	36.0	36.0	32.0	36.0
30	34.06214395427268	36.0	36.0	36.0	32.0	36.0
31	34.07697344665693	36.0	36.0	36.0	32.0	36.0
32	34.039028771841274	36.0	36.0	36.0	32.0	36.0
33	34.01644434080464	36.0	36.0	36.0	32.0	36.0
34	34.02198547062728	36.0	36.0	36.0	32.0	36.0
35	33.99352142253311	36.0	36.0	36.0	32.0	36.0
36	33.95228438384452	36.0	36.0	36.0	32.0	36.0
37	33.941795968932496	36.0	36.0	36.0	32.0	36.0
38	33.382642766796366	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	5.0
8	5.0
9	8.0
10	19.0
11	31.0
12	16.0
13	9.0
14	121.0
15	344.0
16	575.0
17	628.0
18	758.0
19	980.0
20	1387.0
21	2131.0
22	3478.0
23	5797.0
24	9124.0
25	14529.0
26	21912.0
27	31730.0
28	45243.0
29	61045.0
30	81978.0
31	112180.0
32	152960.0
33	230785.0
34	513514.0
35	1054438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07394229735013	16.892980859683778	11.40813040887649	26.6249464340896
2	16.319144380282253	18.844560199897117	38.73160194949168	26.104693470328943
3	19.614504350554775	22.59926461810281	28.309516987799345	29.47671404354307
4	13.079083544575779	15.219389456038975	35.49887749472853	36.20264950465671
5	14.69233832084395	36.18048314253242	34.29978607902201	14.827392457601613
6	35.01103924035361	34.88911521096054	16.666801664135107	13.433043884550733
7	30.604020665626198	29.97999344340847	20.50354452407373	18.9124413668916
8	27.61902082256907	34.5692417351743	18.906060559554323	18.905676882702313
9	27.386903932128504	14.552887015411429	18.3095475304671	39.75066152199297
10	15.770758756049846	27.071591207991062	32.48630165309515	24.671348382863947
11	37.18201610878224	20.99099748175047	22.574975156426056	19.252011253041232
12	24.865997488985002	23.053036132610583	29.40901620649155	22.67195017191287
13	30.226041575618297	19.831198981821593	24.62081594975622	25.321943492803893
14	23.175386709085537	19.57973086301112	25.19129208010072	32.05359034780262
15	24.809671997073124	28.058274865446396	21.834056666447776	25.297996471032704
16	25.151090215834635	26.025676063429966	24.417287406739767	24.40594631399563
17	24.002890974446004	26.15841516610311	25.483300145401515	24.355393714049367
18	24.307145421229492	25.331452353484373	26.510688586988497	23.850713638297634
19	25.63307707262501	24.90134417495762	25.23521356385428	24.230365188563084
20	25.948164721451032	24.78154694097504	25.121035955224798	24.14925238234913
21	25.818900702734066	24.62536553213677	24.726322534600584	24.829411230528578
22	25.89110495949126	25.0590161851648	24.86524442314637	24.184634432197576
23	24.56234222427059	25.133479173070782	25.44820991030899	24.855968692349638
24	25.064424747971753	24.774961072273396	25.539317430241343	24.62129674951351
25	24.939709925434737	24.85549995565652	25.496348485158578	24.708441633750162
26	24.400101821834813	25.547345660824988	25.701656054540344	24.350896462799852
27	24.82525070315408	25.484256468655126	25.026700029801706	24.663792798389085
28	24.540572250542617	25.25962650618333	25.257580104886596	24.94222113838746
29	24.794017742293757	25.220371703910814	25.13974816976903	24.845862384026393
30	24.628227351519023	25.141730195501978	25.368745774099676	24.861296678879324
31	25.273984107941168	24.731387711494072	25.10097741533629	24.89365076522847
32	24.8400487900752	25.376210958666995	24.99114283631322	24.792597414944584
33	24.464797253308763	25.278819601511483	25.59559465242785	24.660788492751905
34	24.95013656484208	25.06312339953083	25.559071649002345	24.427668386624745
35	24.824960591882846	25.257309315665307	25.58954415350682	24.328185938945026
36	24.45095848824914	25.3728638673807	25.48743050107446	24.6887471432957
37	25.00085270537835	25.061437422510398	24.89844278943805	25.0392670826732
38	24.672819260486474	25.524169188798112	25.053579749732368	24.749431800983047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	47.0
2	78.0
3	78.0
4	225.5
5	373.0
6	373.0
7	467.0
8	561.0
9	593.5
10	626.0
11	626.0
12	907.0
13	1188.0
14	1885.5
15	2583.0
16	2583.0
17	3993.0
18	5403.0
19	5403.0
20	7302.5
21	9202.0
22	11168.5
23	13135.0
24	13135.0
25	17375.5
26	21616.0
27	21616.0
28	28601.5
29	35587.0
30	46679.5
31	57772.0
32	57772.0
33	72679.0
34	87586.0
35	87586.0
36	99892.0
37	112198.0
38	125676.0
39	139154.0
40	139154.0
41	149180.5
42	159207.0
43	171990.0
44	184773.0
45	184773.0
46	194733.5
47	204694.0
48	204694.0
49	213447.5
50	222201.0
51	223848.0
52	225495.0
53	225495.0
54	218192.5
55	210890.0
56	210890.0
57	198112.0
58	185334.0
59	168241.0
60	151148.0
61	151148.0
62	132878.0
63	114608.0
64	96183.5
65	77759.0
66	77759.0
67	64109.0
68	50459.0
69	50459.0
70	40322.0
71	30185.0
72	23571.0
73	16957.0
74	16957.0
75	13392.5
76	9828.0
77	9828.0
78	8019.5
79	6211.0
80	4785.5
81	3360.0
82	3360.0
83	2834.5
84	2309.0
85	2309.0
86	1665.0
87	1021.0
88	794.5
89	568.0
90	568.0
91	431.0
92	294.0
93	248.0
94	202.0
95	202.0
96	189.5
97	177.0
98	177.0
99	575.0
100	973.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12034628011481283
2	0.05648559020620864
3	0.012064469455363809
4	0.0036236039000209315
5	2.131531705894666E-4
6	3.4104507294314653E-4
7	0.0
8	2.984144388252532E-4
9	1.7052253647157326E-4
10	4.263063411789332E-4
11	0.002302054242366239
12	0.002813621851780959
13	0.015602812087148952
14	0.009975568383587036
15	0.024256830813081297
16	0.01185131628477434
17	0.021357947693064552
18	0.00618144194709453
19	0.008654018725932343
20	0.006266703215330317
21	0.007588252872985009
22	0.008014559214163943
23	0.009975568383587036
24	0.0153043976483237
25	0.017180145549511004
26	0.01952483042599514
27	0.009719784578879676
28	0.0063945951176839965
29	0.011169226138888048
30	0.0032825588270777853
31	0.00669300955650925
32	0.0073324690682776495
33	0.009293478237700742
34	0.013854956088315329
35	0.01713751491539311
36	0.01581596525773842
37	0.010870811700062795
38	0.006906162727098717
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2345731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.02973297433966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.85148410359561	31.628291974596696
2	15.931173125805831	15.303399824008604
3	7.074176809496683	10.193124695201753
4	3.7730443907504907	7.2487325835230445
5	2.2153363057083526	5.3201005605766145
6	1.3455072214685737	3.877461153730878
7	0.9194384205736105	3.0912267278549383
8	0.6175555509574293	2.372882256744523
9	0.4387402882588845	1.8965321001143127
>10	1.747305693520432	13.35480141613972
>50	0.05023054909394626	1.6596621038676465
>100	0.03341328722319768	2.983115664787082
>500	0.0020575114339202905	0.6557465927523114
>1k	5.367421131962975E-4	0.414922346101841
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2682	0.11433536070418987	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4104507294314653E-4
2	0.0	0.0	0.0	0.0	3.4104507294314653E-4
3	0.0	0.0	0.0	0.0	3.4104507294314653E-4
4	0.0	0.0	0.0	0.0	3.4104507294314653E-4
5	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	5.115676094147197E-4
6	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	5.968288776505064E-4
7	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	5.968288776505064E-4
8	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	5.968288776505064E-4
9	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	5.968288776505064E-4
10	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	6.820901458862931E-4
11	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	6.820901458862931E-4
12	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	0.0013215496576546926
13	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	0.0015347028282441592
14	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	0.0015347028282441592
15	0.0	4.2630634117893316E-5	0.0	4.2630634117893316E-5	0.001790486632951519
16	0.0	4.2630634117893316E-5	0.0	8.526126823578663E-5	0.0018757479011873059
17	0.0	4.2630634117893316E-5	0.0	8.526126823578663E-5	0.0018757479011873059
18	0.0	4.2630634117893316E-5	0.0	1.2789190235367994E-4	0.0020036398035409857
19	0.0	4.2630634117893316E-5	0.0	1.7052253647157326E-4	0.002046270437658879
20	0.0	4.2630634117893316E-5	0.0	2.1315317058946657E-4	0.0022594236082483458
21	0.0	4.2630634117893316E-5	0.0	2.1315317058946657E-4	0.002302054242366239
22	0.0	4.2630634117893316E-5	0.0	2.5578380470735987E-4	0.002302054242366239
23	0.0	4.2630634117893316E-5	0.0	2.984144388252532E-4	0.002302054242366239
24	0.0	4.2630634117893316E-5	0.0	3.836757070610398E-4	0.002429946144719919
25	0.0	4.2630634117893316E-5	0.0	6.820901458862931E-4	0.002472576778837812
26	0.0	4.2630634117893316E-5	0.0	9.805045847115462E-4	0.0025578380470735987
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2135	0.0	21.902197	1
GGTATCA	730	0.0	21.49839	1
TAGACCG	80	2.807652E-6	17.99398	5
ACGGTAT	200	0.0	16.00079	9
AGCGAAA	380	0.0	15.579051	17
GAGCGAA	370	0.0	15.568003	16
GTCCTAC	1200	0.0	15.480304	1
CGACGGT	200	0.0	15.199129	7
AATAGCG	150	1.9845174E-9	14.92834	5
TAGGACG	1410	0.0	14.747783	4
ACGGACC	380	0.0	14.735997	8
GTAGGAC	1470	0.0	14.691469	3
TATCAAC	3145	0.0	14.655988	2
ACGACGG	235	0.0	14.293397	6
TCAACGC	3235	0.0	14.141459	4
GACGGTA	215	0.0	14.138724	8
TGTAGGA	1640	0.0	14.05277	2
CAAGACG	495	0.0	13.895231	4
GCGCAAG	485	0.0	13.8678875	1
GTATAGA	220	1.8189894E-12	13.830365	1
>>END_MODULE