FastQCFastQC Report
Thu 2 Feb 2017
SRR4062940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345731
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT25610.10917705397592477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7750.021.8857671
GTATCAA22100.020.6352711
TAGGACG15550.016.3618954
CGACGGT1800.016.0020687
GTCCTAC12150.015.9355361
GTAGGAC14350.015.6111073
TTAGGAC9750.015.5910943
TGTAGGA15200.015.5783042
AGGACGT16550.015.3735875
TCTAGCG2300.015.30371728
GGACGTG16750.015.19002156
CTGTAGG15900.014.7937351
TATCAAC31400.014.776462
TAGACCG650.0041597214.771145
CGCGTAA1002.3277027E-514.4000210
GACGTGA8200.014.2457447
GTACCGT1801.1823431E-1014.2240596
TAAGACG901.5338536E-414.2237574
TCAACGC31650.014.2069044
CTAGCGG2500.014.07941929
Produced by FastQC (version 0.11.5)