FastQCFastQC Report
Thu 2 Feb 2017
SRR4062939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492723
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41140.16504039959514155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28900.020.9462871
GGTATCA10500.019.6749421
TATCAAC40800.014.866952
TCAACGC42250.014.0474754
ATCAACG42650.013.9535193
AACGCAG43000.013.8396656
CAACGCA43300.013.7065585
TACCGTC3450.013.448497
GTATTAG4750.013.1487381
ATACCGT3300.013.0888336
GTCCTAG3950.012.97376251
TATACAG3950.012.959715
TCTATAC2353.6379788E-1212.9343793
ATTACAC3750.012.7982263
TATACCG1003.7530463E-412.7977135
CGTCGTA2900.012.68942410
CCTTATA2800.012.5749852
CTTACAC4000.012.3982823
TAGAACC3950.012.1499714
ACGCAGA49000.012.1462317
Produced by FastQC (version 0.11.5)