##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062939_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2492723
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262051178570584	32.0	32.0	32.0	32.0	32.0
2	30.84430801176063	32.0	32.0	32.0	32.0	32.0
3	30.88040749012225	32.0	32.0	32.0	32.0	32.0
4	30.964150850295038	32.0	32.0	32.0	32.0	32.0
5	30.821429817913984	32.0	32.0	32.0	32.0	32.0
6	34.50905575950477	36.0	36.0	36.0	32.0	36.0
7	34.45744392778499	36.0	36.0	36.0	32.0	36.0
8	34.42850569437519	36.0	36.0	36.0	32.0	36.0
9	34.60823565233682	36.0	36.0	36.0	32.0	36.0
10	34.28122980371265	36.0	36.0	36.0	32.0	36.0
11	34.589688866352176	36.0	36.0	36.0	32.0	36.0
12	34.39486296712471	36.0	36.0	36.0	32.0	36.0
13	34.47926865520156	36.0	36.0	36.0	32.0	36.0
14	34.380303788266886	36.0	36.0	36.0	32.0	36.0
15	34.31047212225346	36.0	36.0	36.0	32.0	36.0
16	34.33105322974113	36.0	36.0	36.0	32.0	36.0
17	34.27140119459723	36.0	36.0	36.0	32.0	36.0
18	34.28957970861584	36.0	36.0	36.0	32.0	36.0
19	34.277017943830906	36.0	36.0	36.0	32.0	36.0
20	34.26886019826511	36.0	36.0	36.0	32.0	36.0
21	34.25921131228781	36.0	36.0	36.0	32.0	36.0
22	34.23245904177881	36.0	36.0	36.0	32.0	36.0
23	34.17641711493816	36.0	36.0	36.0	32.0	36.0
24	34.15894144676324	36.0	36.0	36.0	32.0	36.0
25	34.13375974787411	36.0	36.0	36.0	32.0	36.0
26	34.09593765532713	36.0	36.0	36.0	32.0	36.0
27	34.095814898005116	36.0	36.0	36.0	32.0	36.0
28	34.07807806964512	36.0	36.0	36.0	32.0	36.0
29	34.05446654120815	36.0	36.0	36.0	32.0	36.0
30	34.030650016066765	36.0	36.0	36.0	32.0	36.0
31	34.04105510319438	36.0	36.0	36.0	32.0	36.0
32	34.00239376777925	36.0	36.0	36.0	32.0	36.0
33	33.9735453959385	36.0	36.0	36.0	32.0	36.0
34	33.9778535360728	36.0	36.0	36.0	32.0	36.0
35	33.95469612949373	36.0	36.0	36.0	32.0	36.0
36	33.91222771242533	36.0	36.0	36.0	32.0	36.0
37	33.91193726699677	36.0	36.0	36.0	32.0	36.0
38	33.32915891577203	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	2.0
7	6.0
8	2.0
9	6.0
10	19.0
11	24.0
12	16.0
13	19.0
14	231.0
15	538.0
16	714.0
17	837.0
18	1009.0
19	1240.0
20	1806.0
21	2640.0
22	4200.0
23	6653.0
24	10424.0
25	16259.0
26	23989.0
27	34910.0
28	48902.0
29	65651.0
30	89001.0
31	120095.0
32	164382.0
33	245459.0
34	542032.0
35	1111656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.15015401596271	17.217052653818147	11.456345542012077	26.176447788207064
2	16.827880637705686	19.352419975707523	37.67995581433995	26.139743572246843
3	19.16140372416123	23.250430203171447	28.48741407471513	29.100751997952194
4	13.020059807931009	15.504006585772709	35.791123167910726	35.684810438385554
5	14.879518918127424	35.97126025237542	33.99249412788867	15.156726701608486
6	34.38891554432996	35.26486771858487	16.78061199109726	13.56560474598791
7	30.328303889604975	30.22893456588202	20.84573444039666	18.597027104116346
8	27.859106009481245	33.23999452804597	19.32875491783845	19.572144544634334
9	27.296633308113165	14.50543463871106	18.39095860977232	39.80697344340346
10	15.747553567180471	26.682676010175264	32.19827729728797	25.371493125356288
11	36.9933741677592	21.273109776352086	22.48658866724329	19.246927388645418
12	24.68102348495182	23.436369179912223	28.98177047812377	22.900836857012187
13	29.592742352885477	19.691475370108385	24.98766238507963	25.728119891926504
14	23.63469024787632	19.29140824066507	25.30570797992211	31.768193531536497
15	25.172742272541964	26.82761725609032	22.329577008143968	25.67006346322375
16	25.75720980244259	25.846038420182634	23.516674423456532	24.88007735391825
17	24.29587792716284	25.878765773606393	24.95505006185256	24.870306237378205
18	24.77290507387957	25.334253401108093	25.658055741664228	24.234785783348112
19	25.59514161594148	25.04714112166181	25.2346223655134	24.12309489688331
20	25.742475746949033	24.611796725712747	24.9233623247945	24.722365202543717
21	26.431024722705548	24.4243384430589	24.700479465546966	24.444157368688586
22	25.926927434548585	24.22970019289591	24.99963892196542	24.843733450590083
23	24.90380090247211	24.457018231188368	25.25349179353794	25.385689072801583
24	24.96389989467877	24.890355584532823	25.08101710216159	25.064727418626813
25	25.04800782569689	24.4833491286369	25.22535390975893	25.243289135907286
26	24.959715407813285	25.054368149813744	25.35461675871134	24.631299683661627
27	25.32211466901075	24.92067242979274	24.768777534961703	24.9884353662348
28	24.90573016943495	24.701684480211057	25.345435390412902	25.04714995994109
29	24.961985195289778	24.989066982286506	25.242030933418924	24.806916889004796
30	24.83347996484053	25.00033097399026	25.398944012439806	24.7672450487294
31	25.22446621572828	24.790819151240882	24.819022859848026	25.165691773182807
32	25.04857477225122	24.83377458176349	24.761800068925425	25.35585057705987
33	24.75492575756968	24.717212769457294	25.10633859041689	25.421522882556136
34	25.320360692899484	24.61618704675166	25.194860427094095	24.86859183325476
35	25.427089627634174	24.573833202131016	25.261501869332122	24.737575300902687
36	24.807039463574323	24.948311813473957	25.1512127715661	25.093435951385622
37	25.45968161907429	24.67002823686873	24.901764744114914	24.968525399942067
38	24.795010443053464	24.82016524336768	25.199814167408206	25.185010146170654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	87.0
2	136.0
3	136.0
4	369.0
5	602.0
6	602.0
7	719.5
8	837.0
9	817.5
10	798.0
11	798.0
12	1244.0
13	1690.0
14	2587.5
15	3485.0
16	3485.0
17	5472.5
18	7460.0
19	7460.0
20	10158.5
21	12857.0
22	15268.5
23	17680.0
24	17680.0
25	21338.0
26	24996.0
27	24996.0
28	31873.5
29	38751.0
30	47260.5
31	55770.0
32	55770.0
33	69471.0
34	83172.0
35	83172.0
36	94419.5
37	105667.0
38	120202.5
39	134738.0
40	134738.0
41	148498.0
42	162258.0
43	179521.0
44	196784.0
45	196784.0
46	205061.0
47	213338.0
48	213338.0
49	224112.0
50	234886.0
51	237650.5
52	240415.0
53	240415.0
54	232491.0
55	224567.0
56	224567.0
57	213587.0
58	202607.0
59	183018.5
60	163430.0
61	163430.0
62	147905.0
63	132380.0
64	110855.0
65	89330.0
66	89330.0
67	73889.0
68	58448.0
69	58448.0
70	46709.0
71	34970.0
72	27417.5
73	19865.0
74	19865.0
75	15453.5
76	11042.0
77	11042.0
78	9370.5
79	7699.0
80	6042.0
81	4385.0
82	4385.0
83	3938.5
84	3492.0
85	3492.0
86	2412.5
87	1333.0
88	1078.5
89	824.0
90	824.0
91	606.0
92	388.0
93	345.5
94	303.0
95	303.0
96	282.5
97	262.0
98	262.0
99	651.0
100	1040.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12219568720632015
2	0.05684546578179765
3	0.011874564482295064
4	0.003088991436272703
5	3.209341751971639E-4
6	2.808174032975184E-4
7	8.023354379929097E-5
8	4.8140126279574584E-4
9	3.610509470968094E-4
10	4.8140126279574584E-4
11	0.002206422454480502
12	0.0025273566296776656
13	0.013840286305377694
14	0.009226857536918463
15	0.022906676754697573
16	0.011353046447599673
17	0.020299086581220618
18	0.00613786610064576
19	0.008304171783226616
20	0.006017515784946823
21	0.006258216416344696
22	0.007501836345233707
23	0.009467558168316336
24	0.01396063662107663
25	0.01688916096975075
26	0.0182531312143387
27	0.008424522098925554
28	0.005776815153548951
29	0.01095187872860322
30	0.003209341751971639
31	0.005736698381649304
32	0.006980318310538315
33	0.00854487241462449
34	0.011954798026094355
35	0.015605424268962095
36	0.015003672690467413
37	0.00950767494021598
38	0.006298333188244342
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2492723.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.59968617574037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.93701486128145	43.739482251323444
2	13.98219314082767	16.107398739205266
3	4.524003866542799	7.817436089121037
4	2.030931481438297	4.679240639011095
5	1.0063966788704914	2.898406643562383
6	0.6142555978136977	2.1228557799456467
7	0.40780746440214943	1.6442707378781753
8	0.27304440997495444	1.2581817861278055
9	0.19309166650840578	1.0009817454631402
>10	0.9337944309043895	9.172174595641899
>50	0.05126239087326381	2.002225220443635
>100	0.04109254503366025	4.917820640374161
>500	0.0036410439383819373	1.456969797850151
>1k	0.0014704215904942498	1.1825553340521102
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4114	0.16504039959514155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0116771899645487E-5
2	0.0	0.0	0.0	0.0	4.0116771899645487E-5
3	0.0	0.0	0.0	0.0	4.0116771899645487E-5
4	0.0	0.0	0.0	0.0	4.0116771899645487E-5
5	0.0	0.0	0.0	0.0	4.0116771899645487E-5
6	0.0	0.0	0.0	0.0	4.0116771899645487E-5
7	0.0	0.0	0.0	4.0116771899645487E-5	4.0116771899645487E-5
8	0.0	0.0	0.0	4.0116771899645487E-5	4.0116771899645487E-5
9	0.0	0.0	0.0	4.0116771899645487E-5	1.2035031569893646E-4
10	0.0	0.0	0.0	4.0116771899645487E-5	1.2035031569893646E-4
11	0.0	0.0	0.0	4.0116771899645487E-5	1.2035031569893646E-4
12	0.0	0.0	0.0	4.0116771899645487E-5	1.6046708759858195E-4
13	0.0	0.0	0.0	4.0116771899645487E-5	1.6046708759858195E-4
14	0.0	0.0	0.0	4.0116771899645487E-5	1.6046708759858195E-4
15	0.0	0.0	0.0	8.023354379929097E-5	2.4070063139787292E-4
16	0.0	0.0	0.0	8.023354379929097E-5	2.4070063139787292E-4
17	0.0	0.0	0.0	8.023354379929097E-5	2.4070063139787292E-4
18	0.0	0.0	0.0	1.2035031569893646E-4	2.808174032975184E-4
19	0.0	0.0	0.0	1.2035031569893646E-4	2.808174032975184E-4
20	0.0	0.0	0.0	1.2035031569893646E-4	3.209341751971639E-4
21	0.0	0.0	0.0	2.0058385949822745E-4	3.209341751971639E-4
22	0.0	0.0	0.0	3.209341751971639E-4	3.209341751971639E-4
23	0.0	0.0	0.0	4.011677189964549E-4	3.209341751971639E-4
24	0.0	0.0	0.0	5.616348065950368E-4	4.011677189964549E-4
25	0.0	0.0	0.0	6.819851222939733E-4	4.011677189964549E-4
26	0.0	0.0	0.0	9.628025255914917E-4	5.215180346953913E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2890	0.0	20.946287	1
GGTATCA	1050	0.0	19.674942	1
TATCAAC	4080	0.0	14.86695	2
TCAACGC	4225	0.0	14.047475	4
ATCAACG	4265	0.0	13.953519	3
AACGCAG	4300	0.0	13.839665	6
CAACGCA	4330	0.0	13.706558	5
TACCGTC	345	0.0	13.44849	7
GTATTAG	475	0.0	13.148738	1
ATACCGT	330	0.0	13.088833	6
GTCCTAG	395	0.0	12.9737625	1
TATACAG	395	0.0	12.95971	5
TCTATAC	235	3.6379788E-12	12.934379	3
ATTACAC	375	0.0	12.798226	3
TATACCG	100	3.7530463E-4	12.797713	5
CGTCGTA	290	0.0	12.689424	10
CCTTATA	280	0.0	12.574985	2
CTTACAC	400	0.0	12.398282	3
TAGAACC	395	0.0	12.149971	4
ACGCAGA	4900	0.0	12.146231	7
>>END_MODULE