FastQCFastQC Report
Thu 2 Feb 2017
SRR4062939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492723
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39240.1574182129342089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25410.10193671739699918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29150.020.8029541
GGTATCA10250.019.0440921
TACCGTA450.00884542617.7804537
CTAGCGG2750.016.29009229
TCATACG600.002443471715.99951813
TCTAGCG2800.015.42779828
TATCAAC40900.014.748612
TCAACGC41200.014.3313914
ATCAACG41800.014.24052053
ACCCGTT1707.6033757E-1014.11693930
CAACGCA42050.014.0800325
TAGTACG800.001018300414.0004222
AACGCAG42600.013.8985256
CCGGCTA1053.6837264E-513.7152499
GTATTAG4000.013.6001361
CGTCTTA1657.565177E-913.57507615
GTAAACG2850.013.47300827
ATATACG3450.013.4510296
TAGCGGC3300.013.09025230
TAAACGC3350.012.894876528
Produced by FastQC (version 0.11.5)