##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2492723
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17766514771196	32.0	32.0	32.0	32.0	32.0
2	31.20113426160869	32.0	32.0	32.0	32.0	32.0
3	31.319506018117536	32.0	32.0	32.0	32.0	32.0
4	31.43723109226336	32.0	32.0	32.0	32.0	32.0
5	31.331719569322384	32.0	32.0	32.0	32.0	32.0
6	34.8688085278629	36.0	36.0	36.0	36.0	36.0
7	34.90775108184905	36.0	36.0	36.0	36.0	36.0
8	34.83192877828784	36.0	36.0	36.0	32.0	36.0
9	34.968405233954996	36.0	36.0	36.0	36.0	36.0
10	34.79173377868299	36.0	36.0	36.0	32.0	36.0
11	34.99560921931558	36.0	36.0	36.0	36.0	36.0
12	34.89449970975515	36.0	36.0	36.0	32.0	36.0
13	34.94543757970701	36.0	36.0	36.0	36.0	36.0
14	34.88641938955913	36.0	36.0	36.0	32.0	36.0
15	34.85683647962489	36.0	36.0	36.0	32.0	36.0
16	34.883155488997375	36.0	36.0	36.0	32.0	36.0
17	34.853255255397414	36.0	36.0	36.0	32.0	36.0
18	34.8538124773591	36.0	36.0	36.0	32.0	36.0
19	34.84210640331878	36.0	36.0	36.0	32.0	36.0
20	34.84102806449012	36.0	36.0	36.0	32.0	36.0
21	34.837200122115455	36.0	36.0	36.0	32.0	36.0
22	34.81587805785079	36.0	36.0	36.0	32.0	36.0
23	34.76047479001878	36.0	36.0	36.0	32.0	36.0
24	34.74313872821008	36.0	36.0	36.0	32.0	36.0
25	34.730570945909356	36.0	36.0	36.0	32.0	36.0
26	34.66549833254638	36.0	36.0	36.0	32.0	36.0
27	34.65136599614157	36.0	36.0	36.0	32.0	36.0
28	34.62505862063294	36.0	36.0	36.0	32.0	36.0
29	34.595131107628085	36.0	36.0	36.0	32.0	36.0
30	34.57675361442086	36.0	36.0	36.0	32.0	36.0
31	34.57342472468862	36.0	36.0	36.0	32.0	36.0
32	34.55098059431393	36.0	36.0	36.0	32.0	36.0
33	34.522919714705566	36.0	36.0	36.0	32.0	36.0
34	34.52178761940256	36.0	36.0	36.0	32.0	36.0
35	34.49974505791458	36.0	36.0	36.0	32.0	36.0
36	34.47083570858054	36.0	36.0	36.0	32.0	36.0
37	34.46793847531394	36.0	36.0	36.0	32.0	36.0
38	34.057418333284524	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	11.0
21	51.0
22	172.0
23	528.0
24	1523.0
25	3905.0
26	8291.0
27	16152.0
28	28219.0
29	44862.0
30	68541.0
31	100111.0
32	145675.0
33	237399.0
34	578243.0
35	1259037.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40896868758761	18.526945300570432	12.164763485890036	26.89932252595192
2	15.892901056394953	20.414622884291596	36.767944131778776	26.924531927534666
3	18.110997491498253	24.124220781851815	28.76216089794173	29.002620828708203
4	12.591912686508186	16.550321155332792	35.64830211507996	35.209464043079066
5	14.46363231183915	36.54015030161819	33.55822813704382	15.437989249498841
6	34.405950126769156	35.73947334638467	16.465627908469465	13.388948618376714
7	30.118545679200547	30.466038969635456	20.776865339329483	18.63855001183451
8	27.741366327362854	33.54454069619799	19.08576925869746	19.6283237177417
9	27.18387943274077	14.341593594202399	18.150161055629486	40.32436591742734
10	15.464976288734652	27.03830429450511	32.21911848892986	25.277600927830377
11	37.27727090004742	21.1775084831032	22.29168555589701	19.25353506095237
12	24.554943282748617	23.580504156911577	28.912198581787894	22.95235397855191
13	29.608844642675187	19.710091554680474	24.93354643405311	25.747517368591232
14	23.513912513238548	19.29478641804936	25.188067653005554	32.00323341570654
15	25.138172191615354	27.040148464149443	22.1454610079018	25.6762183363334
16	25.753252869754427	25.986531991934918	23.380465210320285	24.879749927990368
17	24.300373527263158	25.930197619230057	24.881184150826225	24.888244702680563
18	24.840465627348085	25.405550476326493	25.53388402963346	24.220099866691967
19	25.524536821780842	25.174076702465538	25.1893210757874	24.11206539996622
20	25.750634948207242	24.634987521678102	24.80692800603998	24.807449524074677
21	26.3495805183015	24.51479689447547	24.60465860772915	24.530963979493876
22	25.83420854792472	24.417002778488737	24.92520224878667	24.823586424799878
23	24.891443884591933	24.42568760229789	25.166565037388878	25.5163034757213
24	24.94617332130365	24.939634287484008	24.977263819525877	25.136928571686468
25	25.064347379569305	24.606173176289357	25.16359639269553	25.16588305144581
26	24.992237401523315	25.070826189202005	25.256245983306602	24.680690425968077
27	25.34229649355203	24.951318277770245	24.664242542890864	25.04214268578686
28	24.835571381176326	24.758747762988506	25.347060222896804	25.058620632938357
29	24.88499524415669	25.000451313683868	25.13765067358066	24.97690276857878
30	24.921902674304366	24.99411286372373	25.318818015479454	24.76516644649245
31	25.279583812561608	24.745148177314526	24.809736180072957	25.165531830050913
32	25.094525143788537	24.844196487134752	24.661304124044268	25.399974245032443
33	24.783058526759692	24.765527497439546	25.01509393542724	25.436320040373516
34	25.27276396133866	24.637595111851578	25.198026415289625	24.89161451152013
35	25.41794655884348	24.625158912562686	25.184587296703242	24.772307231890586
36	24.808492560144067	25.04899260768244	25.03226391380029	25.1102509183732
37	25.559919814596327	24.745108060542627	24.771464779680695	24.92350734518035
38	24.86694248328833	24.75140599481931	25.13179383636904	25.24985768552332
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	44.5
2	69.0
3	69.0
4	264.0
5	459.0
6	459.0
7	542.0
8	625.0
9	684.5
10	744.0
11	744.0
12	1164.5
13	1585.0
14	2544.5
15	3504.0
16	3504.0
17	5541.0
18	7578.0
19	7578.0
20	10047.5
21	12517.0
22	15126.0
23	17735.0
24	17735.0
25	21446.0
26	25157.0
27	25157.0
28	32136.5
29	39116.0
30	47823.5
31	56531.0
32	56531.0
33	70509.0
34	84487.0
35	84487.0
36	95338.0
37	106189.0
38	120970.5
39	135752.0
40	135752.0
41	149145.5
42	162539.0
43	180209.5
44	197880.0
45	197880.0
46	205763.0
47	213646.0
48	213646.0
49	224648.0
50	235650.0
51	237767.5
52	239885.0
53	239885.0
54	232497.5
55	225110.0
56	225110.0
57	213863.5
58	202617.0
59	182426.0
60	162235.0
61	162235.0
62	147442.0
63	132649.0
64	110330.5
65	88012.0
66	88012.0
67	73402.5
68	58793.0
69	58793.0
70	46846.5
71	34900.0
72	27342.0
73	19784.0
74	19784.0
75	15223.5
76	10663.0
77	10663.0
78	9113.0
79	7563.0
80	5832.0
81	4101.0
82	4101.0
83	3515.5
84	2930.0
85	2930.0
86	1938.5
87	947.0
88	730.0
89	513.0
90	513.0
91	325.5
92	138.0
93	93.0
94	48.0
95	48.0
96	38.0
97	28.0
98	28.0
99	26.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009186740765018816
2	0.0
3	0.0
4	1.6046708759858195E-4
5	8.023354379929097E-5
6	1.2035031569893646E-4
7	5.215180346953913E-4
8	0.0028081740329751843
9	0.0042523778213624215
10	0.0010430360693907826
11	0.0048541293998571045
12	2.808174032975184E-4
13	2.0058385949822745E-4
14	1.2035031569893646E-4
15	0.0
16	4.0116771899645487E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.6046708759858195E-4
22	4.0116771899645487E-5
23	1.2035031569893646E-4
24	0.0
25	1.2035031569893646E-4
26	4.0116771899645487E-5
27	4.0116771899645487E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.6046708759858195E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2492723.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.60072492868823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05685705540114	41.17614938220542
2	14.932792553170351	16.605481823319774
3	4.990316944237454	8.323957191705562
4	2.1798915710561766	4.848142064666419
5	1.1353262010382175	3.1562479904129255
6	0.6435301582442968	2.146844598711381
7	0.41567112460818356	1.6178131102096673
8	0.3017712706263027	1.3422961127579034
9	0.2178658268122815	1.09021481171558
>10	1.0241430365664437	9.761586738351516
>50	0.0528276374032345	2.028362024052077
>100	0.04305109381625257	4.994882039203881
>500	0.0045755878321760805	1.7897260221687936
>1k	0.0013799391874774662	1.118296090519166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3924	0.1574182129342089	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2541	0.10193671739699918	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.0116771899645487E-5
7	0.0	0.0	0.0	4.0116771899645487E-5	4.0116771899645487E-5
8	0.0	0.0	0.0	4.0116771899645487E-5	4.0116771899645487E-5
9	0.0	0.0	0.0	8.023354379929097E-5	1.2035031569893646E-4
10	0.0	0.0	0.0	8.023354379929097E-5	1.2035031569893646E-4
11	0.0	0.0	0.0	8.023354379929097E-5	2.0058385949822745E-4
12	0.0	0.0	0.0	8.023354379929097E-5	2.4070063139787292E-4
13	0.0	0.0	0.0	8.023354379929097E-5	2.4070063139787292E-4
14	0.0	0.0	0.0	8.023354379929097E-5	2.4070063139787292E-4
15	0.0	0.0	0.0	8.023354379929097E-5	2.808174032975184E-4
16	0.0	0.0	0.0	8.023354379929097E-5	2.808174032975184E-4
17	0.0	0.0	0.0	8.023354379929097E-5	2.808174032975184E-4
18	0.0	0.0	4.0116771899645487E-5	1.2035031569893646E-4	2.808174032975184E-4
19	0.0	0.0	4.0116771899645487E-5	1.2035031569893646E-4	2.808174032975184E-4
20	0.0	0.0	4.0116771899645487E-5	1.6046708759858195E-4	3.209341751971639E-4
21	0.0	0.0	4.0116771899645487E-5	2.0058385949822745E-4	3.209341751971639E-4
22	0.0	0.0	4.0116771899645487E-5	4.011677189964549E-4	3.209341751971639E-4
23	0.0	0.0	4.0116771899645487E-5	4.011677189964549E-4	3.610509470968094E-4
24	0.0	0.0	4.0116771899645487E-5	6.017515784946824E-4	3.610509470968094E-4
25	0.0	0.0	4.0116771899645487E-5	6.819851222939733E-4	3.610509470968094E-4
26	0.0	0.0	4.0116771899645487E-5	9.226857536918463E-4	3.610509470968094E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2915	0.0	20.802954	1
GGTATCA	1025	0.0	19.044092	1
TACCGTA	45	0.008845426	17.780453	7
CTAGCGG	275	0.0	16.290092	29
TCATACG	60	0.0024434717	15.999518	13
TCTAGCG	280	0.0	15.427798	28
TATCAAC	4090	0.0	14.74861	2
TCAACGC	4120	0.0	14.331391	4
ATCAACG	4180	0.0	14.2405205	3
ACCCGTT	170	7.6033757E-10	14.116939	30
CAACGCA	4205	0.0	14.080032	5
TAGTACG	80	0.0010183004	14.000422	2
AACGCAG	4260	0.0	13.898525	6
CCGGCTA	105	3.6837264E-5	13.715249	9
GTATTAG	400	0.0	13.600136	1
CGTCTTA	165	7.565177E-9	13.575076	15
GTAAACG	285	0.0	13.473008	27
ATATACG	345	0.0	13.451029	6
TAGCGGC	330	0.0	13.090252	30
TAAACGC	335	0.0	12.8948765	28
>>END_MODULE