FastQCFastQC Report
Thu 2 Feb 2017
SRR4062938_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062938_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602094
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36970.23076049220582562No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23960.14955427084802764No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16520.10311504818069353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT550.001365124517.45423931
ACCGTCG2600.016.6145748
TCGTTTA3550.016.22506530
CGTTTAT3600.015.99971931
TCTAGCG4000.015.19973428
ATAAGGC1704.7293724E-1115.056683
CTAGCGG4000.014.7997429
GATATAC2100.014.4922241
CATCGTT4000.014.39974728
ATCGTTT3900.014.35872329
TAATATA4700.014.2944984
CGGTCGA901.5350098E-414.22197210
TGTAATA2150.014.1441442
GTCCTAA1707.4578566E-1014.1332841
TACCGTC2750.013.9629557
TCCTATT4250.013.5573592
TATTAGG1902.764864E-1013.4780762
CGGTCCA4650.013.41911910
CGCCGGT4550.013.3619857
GTATTAC1801.7571438E-913.34811
Produced by FastQC (version 0.11.5)