##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062938_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1602094
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.279551012612245	32.0	32.0	32.0	32.0	32.0
2	30.923075674710724	32.0	32.0	32.0	32.0	32.0
3	30.97584161728338	32.0	32.0	32.0	32.0	32.0
4	31.031980645330425	32.0	32.0	32.0	32.0	32.0
5	30.95455322846225	32.0	32.0	32.0	32.0	32.0
6	34.61625659917583	36.0	36.0	36.0	32.0	36.0
7	34.55637496925898	36.0	36.0	36.0	32.0	36.0
8	34.539040156195576	36.0	36.0	36.0	32.0	36.0
9	34.66755883237813	36.0	36.0	36.0	32.0	36.0
10	34.406379400959	36.0	36.0	36.0	32.0	36.0
11	34.6478159209135	36.0	36.0	36.0	32.0	36.0
12	34.48690214182189	36.0	36.0	36.0	32.0	36.0
13	34.559638198507706	36.0	36.0	36.0	32.0	36.0
14	34.46863417502344	36.0	36.0	36.0	32.0	36.0
15	34.41972381146175	36.0	36.0	36.0	32.0	36.0
16	34.42399634478377	36.0	36.0	36.0	32.0	36.0
17	34.36380948933084	36.0	36.0	36.0	32.0	36.0
18	34.382419508468296	36.0	36.0	36.0	32.0	36.0
19	34.37468088638994	36.0	36.0	36.0	32.0	36.0
20	34.35018981408082	36.0	36.0	36.0	32.0	36.0
21	34.33863992999162	36.0	36.0	36.0	32.0	36.0
22	34.311326925885744	36.0	36.0	36.0	32.0	36.0
23	34.26066510454443	36.0	36.0	36.0	32.0	36.0
24	34.24884432498967	36.0	36.0	36.0	32.0	36.0
25	34.22111374238965	36.0	36.0	36.0	32.0	36.0
26	34.183996694326304	36.0	36.0	36.0	32.0	36.0
27	34.17897139618524	36.0	36.0	36.0	32.0	36.0
28	34.15908367424134	36.0	36.0	36.0	32.0	36.0
29	34.13716173957334	36.0	36.0	36.0	32.0	36.0
30	34.098099112786144	36.0	36.0	36.0	32.0	36.0
31	34.118020540617465	36.0	36.0	36.0	32.0	36.0
32	34.07502680866416	36.0	36.0	36.0	32.0	36.0
33	34.037161365063476	36.0	36.0	36.0	32.0	36.0
34	34.04378020265977	36.0	36.0	36.0	32.0	36.0
35	34.00406093525099	36.0	36.0	36.0	32.0	36.0
36	33.95649568627059	36.0	36.0	36.0	32.0	36.0
37	33.95851866369888	36.0	36.0	36.0	32.0	36.0
38	33.380900246802	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	5.0
9	3.0
10	10.0
11	14.0
12	10.0
13	6.0
14	259.0
15	504.0
16	559.0
17	679.0
18	847.0
19	1113.0
20	1454.0
21	1940.0
22	2785.0
23	4257.0
24	6422.0
25	9684.0
26	14115.0
27	20140.0
28	28748.0
29	39537.0
30	53352.0
31	72028.0
32	98839.0
33	149894.0
34	340501.0
35	754387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.89697488959006	16.835867121867448	11.286279671010572	25.980878317531918
2	16.907167177519476	19.45000262296065	37.19476856743882	26.448061632081053
3	19.20314576636854	23.54580407378992	28.340113827401105	28.910936332440436
4	12.991354826628381	15.063886894915889	35.4317905183983	36.51296776005742
5	14.910932136898419	36.24933134343637	33.38183239050417	15.45790412916104
6	34.443490826017346	34.9248011185424	16.794864192599018	13.836843862841237
7	30.448126574503835	30.27522763986088	20.594697433106212	18.68194835252907
8	28.643477359872016	32.45312838425331	19.291749709285813	19.61164454658886
9	27.96628154473219	14.034230286688013	18.143737243226035	39.85575092535375
10	16.117329751329986	26.351006783044323	31.309488959061422	26.222174506564265
11	37.84680262461425	20.92063737778266	21.815233348979817	19.417326648623277
12	25.00471267653811	23.30858605971554	28.58316344810625	23.10353781564009
13	30.010618819344604	19.24216214394025	24.875317831064013	25.87190120565113
14	24.154656319290467	19.068548870378137	24.667031221909273	32.109763588422126
15	25.816664367068338	26.366882287827714	22.192580642543152	25.623872702560796
16	26.239506394794272	25.31131236859584	23.335880264281542	25.113300972328346
17	24.410861119487173	25.27601984533524	24.97666668539575	25.336452349781837
18	25.33988424585023	24.475785510257076	25.615911451571023	24.568418792321676
19	26.02108493336754	24.644428173086467	24.943180293096678	24.391306600449315
20	26.242122425743563	23.80175978267383	24.839514711463558	25.11660308011905
21	27.11399944694098	23.823235844716507	24.200139950149783	24.862624758192734
22	26.245593753257673	23.79395088724191	24.525854454499317	25.434600905001105
23	24.924201517530253	23.70243672535574	24.994491071790854	26.378870685323154
24	25.23999955052757	24.729910767236955	24.660055260138414	25.370034422097063
25	25.508054140002134	23.995175569202303	24.746558191887242	25.75021209890832
26	25.08415469480813	24.66818827677655	25.215193793029396	25.032463235385926
27	25.71704304911293	24.493854719309645	24.471070144653957	25.318032086923463
28	25.032974015913666	24.2644438049736	25.123796908775738	25.578785270336997
29	25.009145218580088	24.621425405604487	25.025562914734103	25.343866461081323
30	25.12466831580268	24.72137279025449	25.193579979663568	24.960378914279257
31	25.512216223504364	24.412721776219733	24.172273688849437	25.902788311426466
32	25.114450543757123	24.45870854093815	24.27050652870596	26.15633438659877
33	24.897533180772317	24.06556378286398	24.96226549361978	26.07463754274393
34	25.52121893879712	24.33551530103289	25.021692745820946	25.121573014349046
35	26.00366450146861	24.181714324954505	24.9548804354951	24.859740738081786
36	25.290939759457338	24.82124106829609	24.381632059370315	25.506187112876262
37	25.876368330636623	24.501233495135935	24.3928032799984	25.22959489422904
38	25.17318868171859	24.580246065879738	24.7671987066248	25.479366545776866
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	407.0
1	369.5
2	332.0
3	332.0
4	689.5
5	1047.0
6	1047.0
7	1168.0
8	1289.0
9	1190.5
10	1092.0
11	1092.0
12	1452.0
13	1812.0
14	2418.0
15	3024.0
16	3024.0
17	4324.0
18	5624.0
19	5624.0
20	6912.5
21	8201.0
22	8556.0
23	8911.0
24	8911.0
25	10586.0
26	12261.0
27	12261.0
28	15972.5
29	19684.0
30	24475.0
31	29266.0
32	29266.0
33	37353.5
34	45441.0
35	45441.0
36	52610.5
37	59780.0
38	69878.5
39	79977.0
40	79977.0
41	88258.5
42	96540.0
43	109676.5
44	122813.0
45	122813.0
46	132887.0
47	142961.0
48	142961.0
49	149230.5
50	155500.0
51	157013.5
52	158527.0
53	158527.0
54	152173.0
55	145819.0
56	145819.0
57	139542.5
58	133266.0
59	121507.0
60	109748.0
61	109748.0
62	101249.5
63	92751.0
64	77056.0
65	61361.0
66	61361.0
67	51411.5
68	41462.0
69	41462.0
70	33291.0
71	25120.0
72	19828.0
73	14536.0
74	14536.0
75	11190.0
76	7844.0
77	7844.0
78	6969.5
79	6095.0
80	4704.0
81	3313.0
82	3313.0
83	3162.5
84	3012.0
85	3012.0
86	2038.5
87	1065.0
88	889.5
89	714.0
90	714.0
91	520.5
92	327.0
93	293.5
94	260.0
95	260.0
96	230.5
97	201.0
98	201.0
99	456.0
100	711.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11990557358057642
2	0.053055563531228506
3	0.012171570457164186
4	0.002746405641616534
5	6.241831003673942E-5
6	5.617647903306547E-4
7	1.2483662007347883E-4
8	3.1209155018369707E-4
9	2.4967324014695766E-4
10	6.866014104041335E-4
11	0.0018725493011021825
12	0.0019973859211756613
13	0.01379444651811941
14	0.008863400025216997
15	0.022782683163409888
16	0.010361439466098743
17	0.019162421181279
18	0.004868628182865675
19	0.006054576073563724
20	0.004868628182865675
21	0.005929739453490244
22	0.006678759173931118
23	0.009300328195474173
24	0.013482354967935713
25	0.015417322579074636
26	0.017601963430360516
27	0.008301635234886343
28	0.004681373252755456
29	0.010236602846025265
30	0.002683987331579795
31	0.00530555635312285
32	0.005742484523380027
33	0.006990850724114814
34	0.010985622566466137
35	0.014917976098780721
36	0.013482354967935713
37	0.008863400025216997
38	0.005243138043086111
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1602094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.08276809482842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9925616027122	45.66196843278896
2	11.77792150161025	13.446327234310223
3	3.468316688802363	5.939433516789854
4	1.4911366479718993	3.404728298955189
5	0.79395357034202	2.266053376694728
6	0.522506778762915	1.7895679968059575
7	0.3461904181327308	1.383305514844565
8	0.2548072095845242	1.1636080682882985
9	0.19221870549506284	0.9875138210336514
>10	1.0321649044546508	10.283982641956703
>50	0.0607496376127477	2.408509738067554
>100	0.05854237134687518	6.915348944353338
>500	0.006614787534680814	2.4574840749755094
>1k	0.002315175637138285	1.8921683401354326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3697	0.23076049220582562	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2396	0.14955427084802764	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1652	0.10311504818069353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.241831003673942E-5
6	0.0	0.0	0.0	0.0	6.241831003673942E-5
7	0.0	0.0	0.0	0.0	6.241831003673942E-5
8	0.0	0.0	0.0	0.0	6.241831003673942E-5
9	0.0	0.0	0.0	0.0	6.241831003673942E-5
10	0.0	0.0	0.0	0.0	6.241831003673942E-5
11	0.0	0.0	0.0	0.0	6.241831003673942E-5
12	0.0	6.241831003673942E-5	0.0	0.0	6.241831003673942E-5
13	0.0	6.241831003673942E-5	0.0	6.241831003673942E-5	6.241831003673942E-5
14	0.0	6.241831003673942E-5	0.0	6.241831003673942E-5	6.241831003673942E-5
15	0.0	6.241831003673942E-5	0.0	6.241831003673942E-5	6.241831003673942E-5
16	0.0	6.241831003673942E-5	0.0	6.241831003673942E-5	6.241831003673942E-5
17	0.0	6.241831003673942E-5	0.0	6.241831003673942E-5	6.241831003673942E-5
18	0.0	6.241831003673942E-5	0.0	1.8725493011021826E-4	6.241831003673942E-5
19	0.0	6.241831003673942E-5	0.0	2.4967324014695766E-4	6.241831003673942E-5
20	0.0	6.241831003673942E-5	0.0	3.745098602204365E-4	6.241831003673942E-5
21	0.0	6.241831003673942E-5	0.0	4.993464802939153E-4	6.241831003673942E-5
22	0.0	6.241831003673942E-5	0.0	8.114380304776125E-4	6.241831003673942E-5
23	0.0	6.241831003673942E-5	0.0	0.0018101309910654431	1.2483662007347883E-4
24	0.0	6.241831003673942E-5	0.0	0.0026215690215430553	1.2483662007347883E-4
25	0.0	6.241831003673942E-5	0.0	0.00318333381187371	1.2483662007347883E-4
26	0.0	6.241831003673942E-5	0.0	0.004306863392535019	3.1209155018369707E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCGT	55	0.0013651245	17.454239	31
ACCGTCG	260	0.0	16.614574	8
TCGTTTA	355	0.0	16.225065	30
CGTTTAT	360	0.0	15.999719	31
TCTAGCG	400	0.0	15.199734	28
ATAAGGC	170	4.7293724E-11	15.05668	3
CTAGCGG	400	0.0	14.79974	29
GATATAC	210	0.0	14.492224	1
CATCGTT	400	0.0	14.399747	28
ATCGTTT	390	0.0	14.358723	29
TAATATA	470	0.0	14.294498	4
CGGTCGA	90	1.5350098E-4	14.221972	10
TGTAATA	215	0.0	14.144144	2
GTCCTAA	170	7.4578566E-10	14.133284	1
TACCGTC	275	0.0	13.962955	7
TCCTATT	425	0.0	13.557359	2
TATTAGG	190	2.764864E-10	13.478076	2
CGGTCCA	465	0.0	13.419119	10
CGCCGGT	455	0.0	13.361985	7
GTATTAC	180	1.7571438E-9	13.3481	1
>>END_MODULE