FastQCFastQC Report
Thu 2 Feb 2017
SRR4062938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602094
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42280.26390461483533423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28180.17589479768353167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18200.11360132426686574No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA16650.10392648621117112No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16080.10036864253907699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA550.00136519617.45410212
CTATAGA1505.456968E-1217.0688971
CGTCTTA753.235681E-517.06623515
CTAGCGG3550.016.67563429
TCTAGCG3650.016.65711228
CGTCGTA3150.015.74563210
TAGCGGC3750.015.3596130
ATACCGT3500.015.0867436
TCGATTA650.00416323314.76885613
TAGGACG1452.0294465E-814.3458074
ACCGTCG3250.014.2778978
AACCGCG2500.014.0809617
CCGTCGT3300.014.0615669
GTCTAGT800.001017247314.001831
CTTACAC2300.013.9139933
CCTTATA1503.1728632E-813.8663152
GTATCAA39150.013.774471
GTTTTCG3950.013.77180428
TACCGTC3650.013.5899697
CGTATTG5100.013.48985427
Produced by FastQC (version 0.11.5)