##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062938_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1602094
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205400557020997	32.0	32.0	32.0	32.0	32.0
2	31.315241178108153	32.0	32.0	32.0	32.0	32.0
3	31.386335633239998	32.0	32.0	32.0	32.0	32.0
4	31.48807061258578	32.0	32.0	32.0	32.0	32.0
5	31.409371110559057	32.0	32.0	32.0	32.0	32.0
6	34.94760045290726	36.0	36.0	36.0	36.0	36.0
7	34.9750289308867	36.0	36.0	36.0	36.0	36.0
8	34.9206569651968	36.0	36.0	36.0	36.0	36.0
9	35.0195344343091	36.0	36.0	36.0	36.0	36.0
10	34.87785673000461	36.0	36.0	36.0	32.0	36.0
11	35.04441562105594	36.0	36.0	36.0	36.0	36.0
12	34.95026696311203	36.0	36.0	36.0	36.0	36.0
13	34.996234303355486	36.0	36.0	36.0	36.0	36.0
14	34.945894560493954	36.0	36.0	36.0	32.0	36.0
15	34.91452686296809	36.0	36.0	36.0	32.0	36.0
16	34.9313691955653	36.0	36.0	36.0	36.0	36.0
17	34.89920004693857	36.0	36.0	36.0	32.0	36.0
18	34.89168862750875	36.0	36.0	36.0	32.0	36.0
19	34.88846721852775	36.0	36.0	36.0	32.0	36.0
20	34.87526137667328	36.0	36.0	36.0	32.0	36.0
21	34.87022172232091	36.0	36.0	36.0	32.0	36.0
22	34.847523928059154	36.0	36.0	36.0	32.0	36.0
23	34.78506941540259	36.0	36.0	36.0	32.0	36.0
24	34.769796903302804	36.0	36.0	36.0	32.0	36.0
25	34.75737253868999	36.0	36.0	36.0	32.0	36.0
26	34.685957253444556	36.0	36.0	36.0	32.0	36.0
27	34.6741458366363	36.0	36.0	36.0	32.0	36.0
28	34.63655877869838	36.0	36.0	36.0	32.0	36.0
29	34.61156648735967	36.0	36.0	36.0	32.0	36.0
30	34.58571344752555	36.0	36.0	36.0	32.0	36.0
31	34.576768279514184	36.0	36.0	36.0	32.0	36.0
32	34.55909328666108	36.0	36.0	36.0	32.0	36.0
33	34.521554915004984	36.0	36.0	36.0	32.0	36.0
34	34.51243185481002	36.0	36.0	36.0	32.0	36.0
35	34.48528675595814	36.0	36.0	36.0	32.0	36.0
36	34.45119512338227	36.0	36.0	36.0	32.0	36.0
37	34.436652905509916	36.0	36.0	36.0	32.0	36.0
38	34.02794592576965	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	11.0
21	31.0
22	121.0
23	345.0
24	999.0
25	2438.0
26	5213.0
27	10337.0
28	17900.0
29	28448.0
30	43127.0
31	62741.0
32	90109.0
33	143894.0
34	357863.0
35	838516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.349603016810065	17.937248003535675	11.933466378935265	26.779682600718996
2	15.930838015746893	20.396743262255523	36.47663620237015	27.195782519627436
3	18.273085099875537	24.213997430862356	28.644948423750417	28.86796904551169
4	12.52307922775874	15.840121341497667	35.441891529189995	36.1949079015536
5	14.321649443352817	36.7293513730797	33.145162699770054	15.803836483797435
6	34.490569523741755	35.36436399135381	16.41346663395945	13.731599850944983
7	30.283811053956494	30.39828673474037	20.513055782863226	18.8048464284399
8	28.47885996423344	32.60281326171698	19.14322541985138	19.775101354198203
9	27.86348836977039	13.882057287245198	17.928418408621052	40.32603593436336
10	15.823574133094642	26.605009244244037	31.35721232049875	26.214204302162564
11	37.96747749260469	20.848873522264576	21.764212757696647	19.419436227434087
12	24.91303520100369	23.261437439336863	28.60484930574845	23.220678053910998
13	30.011672253119364	19.234749608324126	24.936863721763448	25.81671441679306
14	24.064567996634402	19.057620838727317	24.74211875208321	32.135692412555066
15	25.789123484639475	26.432968352668446	22.130599078456072	25.64730908423601
16	26.3027341750661	25.2502353173226	23.279874064660454	25.167156442950844
17	24.34819679744135	25.337901521383888	24.923880870910196	25.39002081026457
18	25.276357067687666	24.470037338633063	25.715532297106165	24.53807329657311
19	26.055212740326102	24.61041611790569	25.055583505087714	24.278787636680494
20	26.260881071897153	23.747233308407623	24.896853742664288	25.095031877030937
21	27.092060198752506	23.786072344114856	24.242412893633517	24.879454563499124
22	26.124841769386524	23.90287199486671	24.64483937252043	25.32744686322633
23	24.857311565128594	23.606010141739674	25.104051452725564	26.432626840406172
24	25.230604446430732	24.635820370090645	24.730384109796304	25.40319107368232
25	25.422385231310063	23.87103861700826	24.906934183555002	25.799641968126675
26	25.166532259071268	24.553458852550293	25.161788461586475	25.11822042679197
27	25.774362521003784	24.332997106283536	24.551336627359728	25.34130374535295
28	25.113008350321515	24.06469283325448	25.186412282924724	25.63588653349928
29	25.136227961655184	24.319234701584303	25.059391021999954	25.48514631476056
30	25.177361627969397	24.502120349991948	25.296643018449604	25.02387500358905
31	25.484771804900337	24.256192208447196	24.3295961410504	25.92943984560207
32	25.16556456737245	24.358620655217482	24.402001380693015	26.07381339671705
33	24.976874016131386	23.957957523091654	25.050964550145	26.014203910631963
34	25.46960415556141	24.20569579562747	25.130672732061914	25.194027316749207
35	26.049220582562572	24.030425181044308	24.91926191596748	25.001092320425645
36	25.31724106076173	24.625084420764328	24.513542900728673	25.544131617745276
37	25.90054016805506	24.41248765677919	24.522905647234182	25.164066527931574
38	25.41923257936176	24.229165080201287	24.867954065117278	25.483648275319677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	181.5
2	170.0
3	170.0
4	601.5
5	1033.0
6	1033.0
7	1174.5
8	1316.0
9	1249.5
10	1183.0
11	1183.0
12	1522.0
13	1861.0
14	2504.5
15	3148.0
16	3148.0
17	4592.5
18	6037.0
19	6037.0
20	7287.0
21	8537.0
22	8874.5
23	9212.0
24	9212.0
25	10625.5
26	12039.0
27	12039.0
28	15656.5
29	19274.0
30	24084.5
31	28895.0
32	28895.0
33	37154.0
34	45413.0
35	45413.0
36	52813.5
37	60214.0
38	70081.5
39	79949.0
40	79949.0
41	88297.0
42	96645.0
43	110067.5
44	123490.0
45	123490.0
46	133100.0
47	142710.0
48	142710.0
49	149915.0
50	157120.0
51	157542.0
52	157964.0
53	157964.0
54	152192.5
55	146421.0
56	146421.0
57	140236.0
58	134051.0
59	121887.5
60	109724.0
61	109724.0
62	101144.0
63	92564.0
64	76877.5
65	61191.0
66	61191.0
67	51095.5
68	41000.0
69	41000.0
70	33154.5
71	25309.0
72	19886.0
73	14463.0
74	14463.0
75	11046.0
76	7629.0
77	7629.0
78	6833.0
79	6037.0
80	4642.5
81	3248.0
82	3248.0
83	2961.5
84	2675.0
85	2675.0
86	1707.0
87	739.0
88	599.5
89	460.0
90	460.0
91	278.5
92	97.0
93	72.5
94	48.0
95	48.0
96	32.5
97	17.0
98	17.0
99	17.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008551308475033301
2	0.0
3	0.0
4	2.4967324014695766E-4
5	1.2483662007347883E-4
6	6.241831003673942E-5
7	4.3692817025717587E-4
8	0.0024343140914328374
9	0.003869935222277844
10	9.986929605878307E-4
11	0.004057190152388062
12	5.617647903306547E-4
13	2.4967324014695766E-4
14	0.0
15	0.0
16	1.2483662007347883E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	6.241831003673942E-5
22	1.2483662007347883E-4
23	1.2483662007347883E-4
24	0.0
25	1.2483662007347883E-4
26	1.2483662007347883E-4
27	1.2483662007347883E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1602094.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.316633571358885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61114412139672	43.48503853988584
2	12.629995924474805	13.972977131238576
3	3.669042377996041	6.088772183441827
4	1.6198186567071524	3.5841166034048126
5	0.8627072348354504	2.3861029994376435
6	0.5361991398320695	1.7796438803621046
7	0.37821818050907174	1.4645229550872474
8	0.26952111085799685	1.1927200423261926
9	0.20734175588707585	1.0322503141002766
>10	1.0785693071218567	10.516482065513541
>50	0.06541661750739192	2.519338202406279
>100	0.06212652657615988	7.207715421251751
>500	0.00750962132975905	2.7474135919311418
>1k	0.002389424968559698	2.0229060696127674
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4228	0.26390461483533423	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2818	0.17589479768353167	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1820	0.11360132426686574	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1665	0.10392648621117112	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1608	0.10036864253907699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.241831003673942E-5
7	0.0	0.0	0.0	0.0	6.241831003673942E-5
8	0.0	0.0	0.0	0.0	6.241831003673942E-5
9	0.0	0.0	0.0	0.0	6.241831003673942E-5
10	0.0	0.0	0.0	0.0	6.241831003673942E-5
11	0.0	0.0	0.0	0.0	6.241831003673942E-5
12	0.0	0.0	0.0	0.0	6.241831003673942E-5
13	0.0	0.0	0.0	6.241831003673942E-5	6.241831003673942E-5
14	0.0	0.0	0.0	6.241831003673942E-5	1.2483662007347883E-4
15	0.0	0.0	0.0	6.241831003673942E-5	1.8725493011021826E-4
16	0.0	0.0	0.0	6.241831003673942E-5	1.8725493011021826E-4
17	0.0	0.0	0.0	1.2483662007347883E-4	1.8725493011021826E-4
18	0.0	0.0	0.0	1.8725493011021826E-4	2.4967324014695766E-4
19	0.0	0.0	0.0	2.4967324014695766E-4	2.4967324014695766E-4
20	0.0	0.0	0.0	3.745098602204365E-4	2.4967324014695766E-4
21	0.0	0.0	0.0	4.993464802939153E-4	2.4967324014695766E-4
22	0.0	0.0	0.0	8.114380304776125E-4	2.4967324014695766E-4
23	0.0	0.0	0.0	0.0018101309910654431	2.4967324014695766E-4
24	0.0	0.0	0.0	0.002559150711506316	2.4967324014695766E-4
25	0.0	0.0	0.0	0.0029336605717267526	2.4967324014695766E-4
26	0.0	0.0	0.0	0.004057190152388062	2.4967324014695766E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAA	55	0.001365196	17.454102	12
CTATAGA	150	5.456968E-12	17.068897	1
CGTCTTA	75	3.235681E-5	17.066235	15
CTAGCGG	355	0.0	16.675634	29
TCTAGCG	365	0.0	16.657112	28
CGTCGTA	315	0.0	15.745632	10
TAGCGGC	375	0.0	15.35961	30
ATACCGT	350	0.0	15.086743	6
TCGATTA	65	0.004163233	14.768856	13
TAGGACG	145	2.0294465E-8	14.345807	4
ACCGTCG	325	0.0	14.277897	8
AACCGCG	250	0.0	14.080961	7
CCGTCGT	330	0.0	14.061566	9
GTCTAGT	80	0.0010172473	14.00183	1
CTTACAC	230	0.0	13.913993	3
CCTTATA	150	3.1728632E-8	13.866315	2
GTATCAA	3915	0.0	13.77447	1
GTTTTCG	395	0.0	13.771804	28
TACCGTC	365	0.0	13.589969	7
CGTATTG	510	0.0	13.489854	27
>>END_MODULE