FastQCFastQC Report
Thu 2 Feb 2017
SRR4062937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074779
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT35990.1734642581209854No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC34830.16787330120461025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33430.1611255945813988No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA30760.14825675409284556No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT29950.1443527238322732No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC29320.14131625585182808No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG28520.13746042349570725No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27760.13379738275739247No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG27640.13321900790397434No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26730.12883299859888692No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24760.1193380114219394No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC24650.11880783447297277No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC24230.11678352248600936No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC24100.11615694972813971No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24040.11586776230143064No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23640.11393984612337026No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG22970.11071058652511905No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG22410.10801150387583448No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA21690.10454125475532575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA507.2003616E-419.19930632
TCTAGCG6600.019.14989928
CTAGCGG6800.018.8228529
CAAGACG8750.017.5502684
AAGACGG8950.017.3368135
TAGCGGC7600.016.84149730
GTATCAA36100.016.5432221
GTATTAG4850.016.5061861
GCGCAAG9050.016.4532931
AGCGGCG7600.016.4204631
CGCAAGA9350.015.915782
ATACCGT6550.015.8749926
GTCCTAC5100.015.6970591
TACCGTC6600.015.5130957
ACCGTCG6400.015.4979468
ACACCGT1153.3553988E-715.3015816
CGCCGGT8300.015.2268977
TAGGACG4450.015.0976674
CGGTCCA8950.015.0183910
GTTTTCG8250.014.93207428
Produced by FastQC (version 0.11.5)