##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062937_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2074779
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.284783102200283	32.0	32.0	32.0	32.0	32.0
2	30.866356368557806	32.0	32.0	32.0	32.0	32.0
3	30.91512349026089	32.0	32.0	32.0	32.0	32.0
4	30.99251920325008	32.0	32.0	32.0	32.0	32.0
5	30.866694235868014	32.0	32.0	32.0	32.0	32.0
6	34.545956460904996	36.0	36.0	36.0	32.0	36.0
7	34.48345823820272	36.0	36.0	36.0	32.0	36.0
8	34.457829966468715	36.0	36.0	36.0	32.0	36.0
9	34.62678579260731	36.0	36.0	36.0	32.0	36.0
10	34.30796099247197	36.0	36.0	36.0	32.0	36.0
11	34.604043611391866	36.0	36.0	36.0	32.0	36.0
12	34.40025853355948	36.0	36.0	36.0	32.0	36.0
13	34.501550285596686	36.0	36.0	36.0	32.0	36.0
14	34.39454419000771	36.0	36.0	36.0	32.0	36.0
15	34.31766274865901	36.0	36.0	36.0	32.0	36.0
16	34.34150480605404	36.0	36.0	36.0	32.0	36.0
17	34.2573739178968	36.0	36.0	36.0	32.0	36.0
18	34.28854735853795	36.0	36.0	36.0	32.0	36.0
19	34.277102766125935	36.0	36.0	36.0	32.0	36.0
20	34.26386280177311	36.0	36.0	36.0	32.0	36.0
21	34.24573749782507	36.0	36.0	36.0	32.0	36.0
22	34.218907652333094	36.0	36.0	36.0	32.0	36.0
23	34.161754095255446	36.0	36.0	36.0	32.0	36.0
24	34.14120202681828	36.0	36.0	36.0	32.0	36.0
25	34.11507779864747	36.0	36.0	36.0	32.0	36.0
26	34.06297152612399	36.0	36.0	36.0	32.0	36.0
27	34.065944372870554	36.0	36.0	36.0	32.0	36.0
28	34.05445061859601	36.0	36.0	36.0	32.0	36.0
29	34.01665141202991	36.0	36.0	36.0	32.0	36.0
30	33.99271488674216	36.0	36.0	36.0	32.0	36.0
31	34.01144025460061	36.0	36.0	36.0	32.0	36.0
32	33.96173327376072	36.0	36.0	36.0	32.0	36.0
33	33.933349045850186	36.0	36.0	36.0	32.0	36.0
34	33.94868079925621	36.0	36.0	36.0	32.0	36.0
35	33.91349922088087	36.0	36.0	36.0	32.0	36.0
36	33.862564157435564	36.0	36.0	36.0	32.0	36.0
37	33.867425398078545	36.0	36.0	36.0	32.0	36.0
38	33.25144412971213	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	5.0
10	23.0
11	20.0
12	12.0
13	7.0
14	149.0
15	426.0
16	504.0
17	625.0
18	772.0
19	1034.0
20	1522.0
21	2306.0
22	3653.0
23	5738.0
24	8749.0
25	13481.0
26	19815.0
27	28546.0
28	40376.0
29	54493.0
30	74185.0
31	100130.0
32	138007.0
33	208039.0
34	459888.0
35	912265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.11899159793625	16.808277374263398	11.244250483026983	25.82848054477337
2	17.192323068072273	18.930622806751046	37.08157379129891	26.795480333877777
3	19.49718397575192	22.82314866409969	27.7481061007753	29.93156125937309
4	12.668167270764247	14.644385748438344	35.43456466414745	37.25288231664996
5	15.14781120844429	35.986697352724995	33.175211769950955	15.690279668879759
6	35.61059450841128	34.692566367501406	16.279708671743848	13.417130452343462
7	31.474147367020777	29.688415007092324	20.06319709231682	18.77424053357008
8	28.811936926081515	32.49634176670978	18.7863051940165	19.905416113192196
9	27.430961221837926	14.044986061145877	18.163888535437078	40.36016418157912
10	16.357668560852527	25.695137292326383	30.65062633448527	27.296567812335827
11	38.535383209453585	20.718966880943853	21.62245924403548	19.123190665567083
12	25.609116577321533	23.143730786619535	27.7451094746969	23.502043161362028
13	29.65363019694498	18.64219026574268	25.094191916270425	26.609987621041913
14	23.94894793568651	19.56634281119914	24.080974150048107	32.403735103066246
15	25.578882869714413	26.87093506860397	21.68633517765296	25.863846884028664
16	26.67673793195533	25.170723853714712	23.11475891868546	25.037779295644498
17	24.551006742792847	25.58197383967994	24.48756558037335	25.379453837153864
18	25.917969248951998	23.985508957849017	25.632187620049734	24.46433417314925
19	26.125052660398513	24.446475344329112	24.69625674944833	24.732215245824044
20	26.260772241826498	23.67760323252811	24.357328739490452	25.70429578615494
21	27.671371405049346	23.662494709940876	23.651938692191344	25.014195192818438
22	26.509611306057916	23.71004126017044	24.412910191647708	25.367437242123934
23	24.614787642740424	23.567486274264066	25.234578731978193	26.583147351017317
24	25.382768776904204	24.516290750979756	24.57023171960337	25.530708752512666
25	25.453449863335955	24.019176352058157	24.678538200855176	25.848835583750716
26	25.06074057219547	25.153442254998566	25.112562885774942	24.67325428703102
27	25.92589914706655	24.15404378182835	24.279513449548226	25.640543621556876
28	24.904526294733344	24.142761085578083	25.340791601495187	25.611921018193385
29	24.87864861555785	24.3181425983609	25.209322100075486	25.593886686005764
30	24.831097120420992	24.643312111120913	25.429300341782213	25.096290426675882
31	25.67936629433149	24.45116149488059	24.036730944051392	25.832741266736527
32	25.359135808598438	24.25701316811214	23.925099041613628	26.458751981675793
33	24.68871287739587	23.965878920974138	24.89834243704365	26.44706576458634
34	25.944184306704805	24.19083344661135	24.885058967232286	24.97992327945156
35	26.491125147448223	23.80854363334598	25.089457144688946	24.61087407451685
36	24.79865990190289	24.78439123151641	24.517817761120284	25.899131105460416
37	26.201460633699657	24.48429295775191	24.335780898196692	24.97846551035174
38	24.99902392939515	24.191801488929432	24.86690494036329	25.942269641312127
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	92.5
2	126.0
3	126.0
4	334.0
5	542.0
6	542.0
7	712.5
8	883.0
9	874.5
10	866.0
11	866.0
12	1206.0
13	1546.0
14	2147.0
15	2748.0
16	2748.0
17	4047.0
18	5346.0
19	5346.0
20	6846.0
21	8346.0
22	9376.5
23	10407.0
24	10407.0
25	12874.5
26	15342.0
27	15342.0
28	20418.5
29	25495.0
30	32212.5
31	38930.0
32	38930.0
33	52002.0
34	65074.0
35	65074.0
36	74002.5
37	82931.0
38	95562.0
39	108193.0
40	108193.0
41	114557.5
42	120922.0
43	139222.5
44	157523.0
45	157523.0
46	162292.5
47	167062.0
48	167062.0
49	180504.5
50	193947.0
51	198375.0
52	202803.0
53	202803.0
54	194560.0
55	186317.0
56	186317.0
57	181773.5
58	177230.0
59	159592.5
60	141955.0
61	141955.0
62	135398.5
63	128842.0
64	107121.5
65	85401.0
66	85401.0
67	71417.0
68	57433.0
69	57433.0
70	45902.0
71	34371.0
72	26699.5
73	19028.0
74	19028.0
75	14290.0
76	9552.0
77	9552.0
78	9220.0
79	8888.0
80	7067.5
81	5247.0
82	5247.0
83	5365.5
84	5484.0
85	5484.0
86	3690.5
87	1897.0
88	1640.0
89	1383.0
90	1383.0
91	1008.0
92	633.0
93	576.0
94	519.0
95	519.0
96	463.0
97	407.0
98	407.0
99	754.0
100	1101.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11716910572162142
2	0.05234292423434014
3	0.010748132692686787
4	0.0028436763626391052
5	1.9279161780604102E-4
6	2.891874267090615E-4
7	0.0
8	3.373853311605718E-4
9	1.4459371335453076E-4
10	4.337811400635923E-4
11	0.0025062910314785337
12	0.0025544889359300437
13	0.014025590195389483
14	0.009205799750238459
15	0.023231389945627946
16	0.011085518023847359
17	0.02005032825182827
18	0.0056873527252782104
19	0.007952654234499193
20	0.0056873527252782104
21	0.006410321292050864
22	0.0066995087187599256
23	0.010073362030365643
24	0.013929194386486465
25	0.01682106865357708
26	0.018459797404928428
27	0.008386435374562785
28	0.0059765401519872715
29	0.010844528501589807
30	0.003181061693799677
31	0.0060247380564387824
32	0.0070368940499204976
33	0.008049050043402213
34	0.011422903355007931
35	0.015519725233386301
36	0.014652162953259119
37	0.00963958089030205
38	0.0064585191965023745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2074779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.727691590697724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41977812348738	38.996345411357254
2	12.670433652705004	12.601428340042242
3	3.850610547374759	5.744459212072193
4	1.6592214021777203	3.3003700067271478
5	0.8935733205937624	2.2217669250081142
6	0.5305261393546866	1.582910414318003
7	0.3730880364541276	1.2986964769078895
8	0.24048883623389808	0.9567163743396078
9	0.19005088793798602	0.8505712747726583
>10	0.9903463759540767	8.873466797525458
>50	0.07820296333727282	2.7193770029294724
>100	0.08026391393472372	8.684270770353086
>500	0.014962793651511442	5.136417277603271
>1k	0.008453006803126594	7.033203716043565
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3599	0.1734642581209854	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3483	0.16787330120461025	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3343	0.1611255945813988	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3076	0.14825675409284556	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2995	0.1443527238322732	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2932	0.14131625585182808	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2852	0.13746042349570725	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2776	0.13379738275739247	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2764	0.13321900790397434	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2673	0.12883299859888692	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2476	0.1193380114219394	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2465	0.11880783447297277	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2423	0.11678352248600936	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2410	0.11615694972813971	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2404	0.11586776230143064	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2364	0.11393984612337026	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2297	0.11071058652511905	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2241	0.10801150387583448	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2169	0.10454125475532575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.8197904451510254E-5
2	0.0	0.0	0.0	0.0	4.8197904451510254E-5
3	0.0	0.0	0.0	0.0	4.8197904451510254E-5
4	0.0	0.0	0.0	0.0	4.8197904451510254E-5
5	0.0	0.0	0.0	0.0	4.8197904451510254E-5
6	0.0	0.0	0.0	0.0	4.8197904451510254E-5
7	0.0	0.0	0.0	4.8197904451510254E-5	4.8197904451510254E-5
8	0.0	0.0	0.0	4.8197904451510254E-5	4.8197904451510254E-5
9	0.0	0.0	0.0	9.639580890302051E-5	4.8197904451510254E-5
10	0.0	0.0	0.0	9.639580890302051E-5	4.8197904451510254E-5
11	0.0	0.0	0.0	9.639580890302051E-5	4.8197904451510254E-5
12	0.0	0.0	0.0	9.639580890302051E-5	3.373853311605718E-4
13	0.0	0.0	0.0	9.639580890302051E-5	3.373853311605718E-4
14	0.0	0.0	0.0	9.639580890302051E-5	3.373853311605718E-4
15	0.0	0.0	0.0	9.639580890302051E-5	3.8558323561208203E-4
16	0.0	0.0	0.0	1.4459371335453076E-4	4.337811400635923E-4
17	0.0	0.0	0.0	1.4459371335453076E-4	4.337811400635923E-4
18	0.0	0.0	0.0	1.4459371335453076E-4	4.337811400635923E-4
19	0.0	0.0	0.0	1.4459371335453076E-4	4.337811400635923E-4
20	0.0	0.0	0.0	1.4459371335453076E-4	4.337811400635923E-4
21	4.8197904451510254E-5	0.0	0.0	2.409895222575513E-4	4.337811400635923E-4
22	4.8197904451510254E-5	0.0	0.0	3.373853311605718E-4	4.337811400635923E-4
23	4.8197904451510254E-5	0.0	0.0	5.78374853418123E-4	4.337811400635923E-4
24	4.8197904451510254E-5	0.0	0.0	0.0010121559934817153	4.337811400635923E-4
25	4.8197904451510254E-5	0.0	0.0	0.0012531455157392666	4.337811400635923E-4
26	4.8197904451510254E-5	0.0	0.0	0.0014941350379968179	5.78374853418123E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	50	7.2003616E-4	19.199306	32
TCTAGCG	660	0.0	19.149899	28
CTAGCGG	680	0.0	18.82285	29
CAAGACG	875	0.0	17.550268	4
AAGACGG	895	0.0	17.336813	5
TAGCGGC	760	0.0	16.841497	30
GTATCAA	3610	0.0	16.543222	1
GTATTAG	485	0.0	16.506186	1
GCGCAAG	905	0.0	16.453293	1
AGCGGCG	760	0.0	16.42046	31
CGCAAGA	935	0.0	15.91578	2
ATACCGT	655	0.0	15.874992	6
GTCCTAC	510	0.0	15.697059	1
TACCGTC	660	0.0	15.513095	7
ACCGTCG	640	0.0	15.497946	8
ACACCGT	115	3.3553988E-7	15.301581	6
CGCCGGT	830	0.0	15.226897	7
TAGGACG	445	0.0	15.097667	4
CGGTCCA	895	0.0	15.01839	10
GTTTTCG	825	0.0	14.932074	28
>>END_MODULE