FastQCFastQC Report
Thu 2 Feb 2017
SRR4062937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074779
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC37190.17924800665516666No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT35150.16941563414705857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34750.16748771796899814No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA32500.15664318946740835No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC31910.15379951310476925No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30140.1452684840168519No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC29840.14382254688330662No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT28710.13837618368028595No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG28490.13731582978235274No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC27630.13317080999952285No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG27620.1331226120950713No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA25830.12449518719825098No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24900.12001278208426053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23470.11312048174769457No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC22710.10945744100937979No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG22440.10815609758918901No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG22390.10791510806693147No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21920.10564980655771049No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC21820.10516782751319538No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC21780.10497503589538934No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21330.10280613019507139No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG21060.1015047867748806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG507.191754E-419.2027195
TCTAGCG6050.018.5114528
CTAGCGG6150.018.2104529
TAGCGGC6450.017.36345130
CATCGTT5300.016.90479528
TAGGACG4150.016.579464
GATATAC2750.016.2920361
ACCGTCG5600.016.2884148
TACCGTC5700.016.28347
ATCGTTT5550.016.14331829
TAATACG703.6962383E-416.001114
CGTTTAT5500.015.9991831
CAAGACG8250.015.9041324
AAGACGG8050.015.7040875
GTATCAA32600.015.6575261
AGCGGCG7500.015.57253531
CGTCGTA6000.015.46736610
TGTAGGA5500.015.4177632
ATACCGT5950.015.3302726
CGAGCCG7550.015.25749715
Produced by FastQC (version 0.11.5)