##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062937_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2074779
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231471400086466	32.0	32.0	32.0	32.0	32.0
2	31.284038444576506	32.0	32.0	32.0	32.0	32.0
3	31.370696830843187	32.0	32.0	32.0	32.0	32.0
4	31.475733560056277	32.0	32.0	32.0	32.0	32.0
5	31.38284704057637	32.0	32.0	32.0	32.0	32.0
6	34.939715507049186	36.0	36.0	36.0	36.0	36.0
7	34.957658622918395	36.0	36.0	36.0	36.0	36.0
8	34.88593676724123	36.0	36.0	36.0	32.0	36.0
9	35.00508005912919	36.0	36.0	36.0	36.0	36.0
10	34.838251206514045	36.0	36.0	36.0	32.0	36.0
11	35.03044276040966	36.0	36.0	36.0	36.0	36.0
12	34.919839173232425	36.0	36.0	36.0	32.0	36.0
13	34.98564473613816	36.0	36.0	36.0	36.0	36.0
14	34.92024499958791	36.0	36.0	36.0	32.0	36.0
15	34.88985188301983	36.0	36.0	36.0	32.0	36.0
16	34.8951449768867	36.0	36.0	36.0	32.0	36.0
17	34.86872143972924	36.0	36.0	36.0	32.0	36.0
18	34.8727541583947	36.0	36.0	36.0	32.0	36.0
19	34.868115110091246	36.0	36.0	36.0	32.0	36.0
20	34.85769375919074	36.0	36.0	36.0	32.0	36.0
21	34.8431890818251	36.0	36.0	36.0	32.0	36.0
22	34.824181274246556	36.0	36.0	36.0	32.0	36.0
23	34.766743349532646	36.0	36.0	36.0	32.0	36.0
24	34.74092758795033	36.0	36.0	36.0	32.0	36.0
25	34.71828180254379	36.0	36.0	36.0	32.0	36.0
26	34.65566742289179	36.0	36.0	36.0	32.0	36.0
27	34.64583505038368	36.0	36.0	36.0	32.0	36.0
28	34.614983089765225	36.0	36.0	36.0	32.0	36.0
29	34.573304915848865	36.0	36.0	36.0	32.0	36.0
30	34.5578806224663	36.0	36.0	36.0	32.0	36.0
31	34.55849032595761	36.0	36.0	36.0	32.0	36.0
32	34.53674825125953	36.0	36.0	36.0	32.0	36.0
33	34.50901180318482	36.0	36.0	36.0	32.0	36.0
34	34.49327374144427	36.0	36.0	36.0	32.0	36.0
35	34.463861934210826	36.0	36.0	36.0	32.0	36.0
36	34.438152689997345	36.0	36.0	36.0	32.0	36.0
37	34.439247746386485	36.0	36.0	36.0	32.0	36.0
38	33.984000223638276	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	34.0
22	139.0
23	436.0
24	1253.0
25	3210.0
26	6834.0
27	13153.0
28	22978.0
29	36721.0
30	55706.0
31	81977.0
32	119515.0
33	195323.0
34	486044.0
35	1051448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24976356819696	18.075922325042875	11.948795764376067	26.725518342384103
2	16.075832654947828	19.860621299907123	36.43265138118325	27.630894663961804
3	18.361377284038443	23.50284054349885	28.13441817176673	30.00136400069598
4	12.066476669977872	15.575969033720796	35.486339951406734	36.8712143448946
5	14.501689095561504	36.587366654472596	32.82947244019724	16.081471809768654
6	35.507929530705965	35.15218028355832	15.972664036985199	13.367226148750516
7	31.228247578405117	29.850937622453387	20.09250186406294	18.828312935078557
8	28.679855133794952	32.66068744933105	18.6504635275133	20.008993889360703
9	27.285815711884677	13.859855198132548	18.020992944993896	40.833336144988884
10	16.067834351925043	26.045229327729473	30.640363222734194	27.246573097611286
11	38.71566513727197	20.61858303726315	21.5385334681013	19.127218357363578
12	25.33755613886096	23.206135053302397	27.742694838839654	23.713613968996984
13	29.58661561537245	18.65063987630472	25.06034386362672	26.7024006446961
14	23.769338213534173	19.533945318487085	24.085998598404263	32.61071786957448
15	25.463434900777383	27.032469482291848	21.53790837481968	25.96618724211109
16	26.614824925678725	25.275885203993102	22.96243064311521	25.146859227212964
17	24.52882933555815	25.769587989853378	24.293430770216972	25.4081519043715
18	25.887672855759575	24.003905958176748	25.611547061156877	24.496874124906796
19	26.030194059222694	24.48670436706753	24.662626718315543	24.820474855394238
20	26.25633862690918	23.6512900892095	24.28687585521157	25.80549542866975
21	27.58097492840198	23.703789031886785	23.701716520996463	25.013519518714773
22	26.400257569601386	23.781231639610773	24.40471973159551	25.41379105919233
23	24.592341534700612	23.46465353368267	25.204504004287692	26.738500927329024
24	25.28331933184209	24.59707756826149	24.576641656774047	25.542961443122376
25	25.35826707326419	24.005689280641455	24.76793913954209	25.868104506552264
26	25.017652482505365	25.16605383031156	25.096504254188034	24.719789432995032
27	25.936882916204567	24.11013413958788	24.199300262823172	25.753682681384376
28	24.903616240573093	24.163055438675638	25.225771033926986	25.707557286824283
29	24.87710739312476	24.31304731732874	25.188755043308227	25.621090246238275
30	24.780470594699484	24.592450569434142	25.437263438660214	25.18981539720616
31	25.63024784808406	24.357148399901867	24.11018233749233	25.902421414521736
32	25.463627692395193	24.228652786634143	23.951322044420152	26.356397476550512
33	24.60421085812031	23.91126958582095	24.98121486674002	26.503304689318718
34	25.831763286595827	24.157946460803778	24.948536687521898	25.061753565078497
35	26.518438831316494	23.743348086711887	25.06083780489392	24.677375277077704
36	24.825439239552743	24.635346704395985	24.616163938424286	25.923050117626982
37	26.259182303271817	24.406165668729056	24.30605862118327	25.028593406815858
38	25.08435838226626	24.006653238730486	24.8943622429184	26.014626136084857
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	52.5
2	72.0
3	72.0
4	258.0
5	444.0
6	444.0
7	616.5
8	789.0
9	804.0
10	819.0
11	819.0
12	1167.5
13	1516.0
14	2137.5
15	2759.0
16	2759.0
17	4129.0
18	5499.0
19	5499.0
20	6819.5
21	8140.0
22	9223.5
23	10307.0
24	10307.0
25	12868.5
26	15430.0
27	15430.0
28	20417.0
29	25404.0
30	32250.0
31	39096.0
32	39096.0
33	52528.0
34	65960.0
35	65960.0
36	74383.0
37	82806.0
38	95720.5
39	108635.0
40	108635.0
41	115177.5
42	121720.0
43	140003.0
44	158286.0
45	158286.0
46	162539.5
47	166793.0
48	166793.0
49	181041.5
50	195290.0
51	199198.0
52	203106.0
53	203106.0
54	195366.5
55	187627.0
56	187627.0
57	182484.0
58	177341.0
59	159780.0
60	142219.0
61	142219.0
62	135622.0
63	129025.0
64	106796.5
65	84568.0
66	84568.0
67	71265.5
68	57963.0
69	57963.0
70	45978.5
71	33994.0
72	26577.5
73	19161.0
74	19161.0
75	14209.0
76	9257.0
77	9257.0
78	9073.5
79	8890.0
80	6919.0
81	4948.0
82	4948.0
83	4738.5
84	4529.0
85	4529.0
86	2876.0
87	1223.0
88	1035.5
89	848.0
90	848.0
91	507.5
92	167.0
93	114.0
94	61.0
95	61.0
96	42.0
97	23.0
98	23.0
99	27.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008916612323529399
2	0.0
3	0.0
4	2.891874267090615E-4
5	0.0
6	1.4459371335453076E-4
7	4.819790445151026E-4
8	0.0017833224647058794
9	0.003952228165023841
10	9.157601845786949E-4
11	0.004916186254054046
12	6.265727578696334E-4
13	1.4459371335453076E-4
14	4.8197904451510254E-5
15	0.0
16	1.4459371335453076E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	4.8197904451510254E-5
22	0.0
23	1.4459371335453076E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2074779.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.756394966838286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86856676087088	36.70965634766927
2	13.660250546860986	13.047286409237238
3	4.116026200612759	5.89699718790953
4	1.7367206880266086	3.3175807649791147
5	0.9380427694511313	2.239877049684753
6	0.567408123972397	1.6258419885491122
7	0.38840185380588255	1.2984070635344138
8	0.2749646782874174	1.0505057422578823
9	0.19549986091258906	0.8402731716333198
>10	1.0583286642982745	9.140485926103292
>50	0.08412688483068138	2.795233244247519
>100	0.08620843233691	8.996804582382739
>500	0.01571893640911654	5.1857440445610345
>1k	0.009735599324356048	7.855306477250726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3719	0.17924800665516666	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3515	0.16941563414705857	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3475	0.16748771796899814	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3250	0.15664318946740835	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3191	0.15379951310476925	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3014	0.1452684840168519	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2984	0.14382254688330662	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2871	0.13837618368028595	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2849	0.13731582978235274	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2763	0.13317080999952285	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2762	0.1331226120950713	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2583	0.12449518719825098	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2490	0.12001278208426053	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2347	0.11312048174769457	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2271	0.10945744100937979	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2244	0.10815609758918901	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2239	0.10791510806693147	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2192	0.10564980655771049	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2182	0.10516782751319538	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2178	0.10497503589538934	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2133	0.10280613019507139	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2106	0.1015047867748806	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.8197904451510254E-5	0.0
11	0.0	0.0	0.0	4.8197904451510254E-5	0.0
12	0.0	0.0	0.0	4.8197904451510254E-5	1.9279161780604102E-4
13	0.0	0.0	0.0	4.8197904451510254E-5	1.9279161780604102E-4
14	0.0	0.0	0.0	4.8197904451510254E-5	1.9279161780604102E-4
15	0.0	0.0	0.0	4.8197904451510254E-5	2.891874267090615E-4
16	0.0	0.0	0.0	1.4459371335453076E-4	2.891874267090615E-4
17	0.0	0.0	0.0	1.4459371335453076E-4	2.891874267090615E-4
18	0.0	0.0	0.0	1.4459371335453076E-4	2.891874267090615E-4
19	0.0	0.0	0.0	1.4459371335453076E-4	2.891874267090615E-4
20	0.0	0.0	0.0	1.4459371335453076E-4	3.373853311605718E-4
21	0.0	0.0	0.0	2.409895222575513E-4	3.8558323561208203E-4
22	0.0	0.0	0.0	3.373853311605718E-4	3.8558323561208203E-4
23	0.0	0.0	0.0	6.265727578696333E-4	3.8558323561208203E-4
24	0.0	0.0	0.0	0.0010603538979332256	3.8558323561208203E-4
25	0.0	0.0	0.0	0.0013495413246422871	3.8558323561208203E-4
26	0.0	0.0	0.0	0.0013977392290937974	5.78374853418123E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	50	7.191754E-4	19.202719	5
TCTAGCG	605	0.0	18.51145	28
CTAGCGG	615	0.0	18.21045	29
TAGCGGC	645	0.0	17.363451	30
CATCGTT	530	0.0	16.904795	28
TAGGACG	415	0.0	16.57946	4
GATATAC	275	0.0	16.292036	1
ACCGTCG	560	0.0	16.288414	8
TACCGTC	570	0.0	16.2834	7
ATCGTTT	555	0.0	16.143318	29
TAATACG	70	3.6962383E-4	16.00111	4
CGTTTAT	550	0.0	15.99918	31
CAAGACG	825	0.0	15.904132	4
AAGACGG	805	0.0	15.704087	5
GTATCAA	3260	0.0	15.657526	1
AGCGGCG	750	0.0	15.572535	31
CGTCGTA	600	0.0	15.467366	10
TGTAGGA	550	0.0	15.417763	2
ATACCGT	595	0.0	15.330272	6
CGAGCCG	755	0.0	15.257497	15
>>END_MODULE