FastQCFastQC Report
Thu 2 Feb 2017
SRR4062936_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062936_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803631
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT68930.857731969025585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT45780.5696644355431784No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA40950.5095622244537604No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA38310.47671132646699793No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC31760.3952062575983256No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG31180.3879890148588096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT25180.3133278830707128No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA23040.28669874606629164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20830.25919856252434265No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA16750.20842899290843686No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA16580.20631359417444076No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT16490.2051936771976193No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA14640.18217316156295613No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA14250.17732018799672983No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA12790.15915264592829295No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA12570.15641507109606273No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA12450.1549218484603008No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11940.1485756522583126No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA11800.146833559183257No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC11560.1438471139117331No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA11200.1393674460044473No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG11140.13862083468656636No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG10270.12779497057729233No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT10000.12443521964682795No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT9450.11759128256625241No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT9440.11746684734660559No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA9410.11709354168766511No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC9010.112116132901792No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA8960.11149395680355785No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT8940.11124508636426421No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA8650.10763646499450617No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT8560.10651654801768473No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT8390.10440114928368865No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC8350.10390340840510134No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG8220.10228575054969258No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG8200.10203688011039894No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT8160.10153913923181161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC308.4125437E-426.66780917
TAGGACC8750.023.0366864
GACCTAG653.8165672E-722.1520377
GGTTTCG805.2477844E-921.99957320
TGCAGTA400.004479603420.00212
AGGACCT17000.019.3854475
TCGGGGT852.394263E-718.82316424
ATGTACG953.7860445E-818.52595523
ATTTAGA8850.018.4540981
GGACCTG16950.018.2156686
TCCTATA1850.018.164072
CTAGGAC1500.018.1318513
ACTGTTC4800.017.9985318
TAGAAAT9300.017.8898984
TTCGGGG904.3635737E-717.77743323
GTACGAA904.3635737E-717.77743325
ATAGGAC2250.017.7763273
CGCCCTT450.00885535917.775226
TTTAGAA9600.017.6685242
GTCCTAA18950.017.659281
Produced by FastQC (version 0.11.5)