##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062936_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	803631
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.170335639117955	32.0	32.0	32.0	32.0	32.0
2	30.91393438033127	32.0	32.0	32.0	32.0	32.0
3	30.98613169477036	32.0	32.0	32.0	32.0	32.0
4	31.01269488110837	32.0	32.0	32.0	32.0	32.0
5	31.022696984063582	32.0	32.0	32.0	32.0	32.0
6	34.645077156057944	36.0	36.0	36.0	32.0	36.0
7	34.52584830600114	36.0	36.0	36.0	32.0	36.0
8	34.48869568247118	36.0	36.0	36.0	32.0	36.0
9	34.564830127259896	36.0	36.0	36.0	32.0	36.0
10	34.394428537475534	36.0	36.0	36.0	32.0	36.0
11	34.6418505508125	36.0	36.0	36.0	32.0	36.0
12	34.509906910012184	36.0	36.0	36.0	32.0	36.0
13	34.57700860220673	36.0	36.0	36.0	32.0	36.0
14	34.49676032905649	36.0	36.0	36.0	32.0	36.0
15	34.50172280561601	36.0	36.0	36.0	32.0	36.0
16	34.48559724550198	36.0	36.0	36.0	32.0	36.0
17	34.46672166703375	36.0	36.0	36.0	32.0	36.0
18	34.52557205981352	36.0	36.0	36.0	32.0	36.0
19	34.41984567544059	36.0	36.0	36.0	32.0	36.0
20	34.35417374391979	36.0	36.0	36.0	32.0	36.0
21	34.30065913335847	36.0	36.0	36.0	32.0	36.0
22	34.2880700221868	36.0	36.0	36.0	32.0	36.0
23	34.34589880181327	36.0	36.0	36.0	32.0	36.0
24	34.30761008472794	36.0	36.0	36.0	32.0	36.0
25	34.2962790136269	36.0	36.0	36.0	32.0	36.0
26	34.321092640776676	36.0	36.0	36.0	32.0	36.0
27	34.28314363184098	36.0	36.0	36.0	32.0	36.0
28	34.225582387936754	36.0	36.0	36.0	32.0	36.0
29	34.13855488402015	36.0	36.0	36.0	32.0	36.0
30	34.093149716723225	36.0	36.0	36.0	32.0	36.0
31	34.09553762858825	36.0	36.0	36.0	32.0	36.0
32	34.04942940230031	36.0	36.0	36.0	32.0	36.0
33	34.04498457625453	36.0	36.0	36.0	32.0	36.0
34	33.997232560715055	36.0	36.0	36.0	32.0	36.0
35	33.976883669246206	36.0	36.0	36.0	32.0	36.0
36	33.91020007938967	36.0	36.0	36.0	32.0	36.0
37	33.862403267171125	36.0	36.0	36.0	32.0	36.0
38	33.27722424844238	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	6.0
11	12.0
12	3.0
13	5.0
14	124.0
15	301.0
16	381.0
17	488.0
18	563.0
19	666.0
20	855.0
21	1086.0
22	1531.0
23	2188.0
24	3189.0
25	4645.0
26	6743.0
27	9834.0
28	13856.0
29	19246.0
30	26446.0
31	35529.0
32	49132.0
33	73489.0
34	174477.0
35	378829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54416071897973	20.983364748415646	12.817602744760451	23.65487178784417
2	11.77384574778014	20.9995219147315	47.96651909104038	19.260113246447975
3	17.918971557286774	28.419068004903274	30.853032499735544	22.808927938074408
4	9.409325702737782	17.715500238300564	41.70695377217968	31.168220286781974
5	9.315504188505129	42.48433354736271	36.57562952012623	11.624532744005933
6	27.079411021752577	41.837340364770306	19.682092109667522	11.401156503809593
7	25.82304565154903	34.64152079748043	22.29530717456146	17.240126376409073
8	23.424644238378953	41.41095133569264	19.691573713210587	15.472830712717824
9	27.930639722260242	14.576857509052674	22.031158617771858	35.46134415091522
10	15.377302376976354	29.166054856363534	34.97422930567204	20.482413460988074
11	32.21405354822974	22.57855781148116	28.736877995848744	16.470510644440353
12	26.120014036729444	26.11652974218709	33.16003115954834	14.60342506153513
13	29.082070773067702	24.267665265343	27.650007591540287	19.00025637004901
14	19.027624639416196	26.756002678089196	28.619464929725048	25.59690775276956
15	19.80533583924026	38.15293085314929	24.830757933124644	17.210975374485802
16	17.79062083487338	29.51501281194504	34.02538992740935	18.668976425772232
17	16.942931535765492	31.883762147522702	31.58418133662578	19.58912498008603
18	16.99691754782654	27.85195269674121	38.43719550362689	16.713934251805355
19	23.042556818959607	26.23559381303971	30.196338548038042	20.52551081996264
20	23.26623817019979	28.940808999670224	31.824635850594525	15.968316979535457
21	21.645164943334755	26.54412322870558	28.591837268022186	23.21887455993748
22	22.10418115908664	32.547558989075156	28.87661604741775	16.471643804420463
23	18.444003076302764	32.24368499257058	32.256627366649994	17.055684564476667
24	22.24841260556702	28.48212485594598	32.889244547104106	16.380217991382896
25	19.857124188700613	30.606537688473484	32.99174232571453	16.54459579711137
26	15.907761468209616	32.06232373593932	34.70631576065176	17.323599035199294
27	17.37967149138035	32.26199053972829	32.119750463866914	18.238587505024448
28	16.34651760114686	32.44377687957948	30.642471726664546	20.56723379260911
29	19.227519193171226	29.713854053495716	29.761766978909606	21.296859774423456
30	17.55155524362746	29.88673525766422	32.25892354940991	20.30278594929841
31	21.33091085572777	28.41840004479924	31.008542966829893	19.242146132643093
32	17.20297768972692	32.518766115463876	31.549722739355087	18.72853345545412
33	16.77941381438783	32.77046870955538	33.762170718595065	16.68794675746172
34	17.903415490109452	31.557037875912364	34.22957021697602	16.309976417002158
35	17.748063814773378	32.91645561428378	32.11821090635746	17.217269664585388
36	16.542543658774566	31.193746390808258	34.09192735817121	18.171782592245965
37	17.5240399273731	31.576897278233456	29.012679840559315	21.88638295383413
38	18.1711953309233	35.06797662972803	29.253159279976604	17.507668759372063
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	136.0
1	205.0
2	274.0
3	274.0
4	614.0
5	954.0
6	954.0
7	1371.5
8	1789.0
9	2017.5
10	2246.0
11	2246.0
12	2832.5
13	3419.0
14	4524.0
15	5629.0
16	5629.0
17	7837.0
18	10045.0
19	10045.0
20	11767.5
21	13490.0
22	14850.5
23	16211.0
24	16211.0
25	20502.0
26	24793.0
27	24793.0
28	33691.0
29	42589.0
30	55559.5
31	68530.0
32	68530.0
33	81857.5
34	95185.0
35	95185.0
36	98365.5
37	101546.0
38	101434.0
39	101322.0
40	101322.0
41	89544.0
42	77766.0
43	69221.5
44	60677.0
45	60677.0
46	53399.0
47	46121.0
48	46121.0
49	42138.5
50	38156.0
51	31455.5
52	24755.0
53	24755.0
54	22598.0
55	20441.0
56	20441.0
57	17378.5
58	14316.0
59	12159.5
60	10003.0
61	10003.0
62	8698.0
63	7393.0
64	6500.5
65	5608.0
66	5608.0
67	4712.5
68	3817.0
69	3817.0
70	3199.5
71	2582.0
72	2048.0
73	1514.0
74	1514.0
75	1172.0
76	830.0
77	830.0
78	665.5
79	501.0
80	394.0
81	287.0
82	287.0
83	220.5
84	154.0
85	154.0
86	116.0
87	78.0
88	61.5
89	45.0
90	45.0
91	39.0
92	33.0
93	28.5
94	24.0
95	24.0
96	21.5
97	19.0
98	19.0
99	186.0
100	353.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11672023602872462
2	0.053133838789195545
3	0.010701428889627204
4	0.002986445271523871
5	3.733056589404839E-4
6	2.488704392936559E-4
7	0.0
8	3.733056589404839E-4
9	1.2443521964682796E-4
10	6.221760982341398E-4
11	0.002364269173289731
12	0.003110880491170699
13	0.013065698062916936
14	0.00908377103421844
15	0.024389303050778282
16	0.010452558450333549
17	0.02177616343819489
18	0.006221760982341398
19	0.008710465375277957
20	0.005724020103754087
21	0.006470631421635054
22	0.006221760982341398
23	0.00858603015563113
24	0.014061179820091559
25	0.0169231898719686
26	0.018540847727377366
27	0.007466113178809677
28	0.0058484553234009135
29	0.011199169768214516
30	0.0033597509304643548
31	0.005724020103754087
32	0.006843937080575538
33	0.007839418837750162
34	0.010701428889627204
35	0.014683355918325699
36	0.01580327289514715
37	0.008959335814571614
38	0.005724020103754087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	803631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.36117924037262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7463738159054	28.240218796146294
2	14.556757605176932	11.459422905120528
3	5.607628973106647	6.621686673718721
4	2.5854347149057317	4.070630369107447
5	1.4679525393893036	2.8890171509631255
6	0.8837406638177719	2.0871044802322323
7	0.5587284094964937	1.5394546351015859
8	0.390542334288002	1.2297765456690855
9	0.2898370658606309	1.0267495829859579
>10	1.5473758663249924	11.313007563182964
>50	0.1752362505995862	4.817068350151179
>100	0.16355378485007377	12.960719588812053
>500	0.01957593563842036	5.402050096050445
>1k	0.00694629974266529	5.486435768289574
>5k	3.1574089739387683E-4	0.8566574944688089
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6893	0.857731969025585	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4578	0.5696644355431784	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	4095	0.5095622244537604	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	3831	0.47671132646699793	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	3176	0.3952062575983256	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	3118	0.3879890148588096	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2518	0.3133278830707128	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2304	0.28669874606629164	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2083	0.25919856252434265	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	1675	0.20842899290843686	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	1658	0.20631359417444076	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	1649	0.2051936771976193	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	1464	0.18217316156295613	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	1425	0.17732018799672983	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	1279	0.15915264592829295	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1257	0.15641507109606273	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	1245	0.1549218484603008	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1194	0.1485756522583126	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	1180	0.146833559183257	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	1156	0.1438471139117331	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1120	0.1393674460044473	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	1114	0.13862083468656636	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	1027	0.12779497057729233	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	1000	0.12443521964682795	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	945	0.11759128256625241	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	944	0.11746684734660559	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	941	0.11709354168766511	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	901	0.112116132901792	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	896	0.11149395680355785	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	894	0.11124508636426421	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	865	0.10763646499450617	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	856	0.10651654801768473	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	839	0.10440114928368865	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	835	0.10390340840510134	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG	822	0.10228575054969258	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	820	0.10203688011039894	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	816	0.10153913923181161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2443521964682796E-4	0.0
3	0.0	0.0	0.0	1.2443521964682796E-4	0.0
4	0.0	0.0	0.0	1.2443521964682796E-4	0.0
5	0.0	0.0	0.0	1.2443521964682796E-4	0.0
6	0.0	0.0	0.0	1.2443521964682796E-4	0.0
7	0.0	0.0	0.0	2.488704392936559E-4	0.0
8	0.0	0.0	0.0	3.733056589404839E-4	0.0
9	0.0	0.0	0.0	4.977408785873118E-4	0.0
10	0.0	0.0	0.0	4.977408785873118E-4	0.0
11	0.0	0.0	0.0	4.977408785873118E-4	0.0
12	0.0	0.0	0.0	4.977408785873118E-4	0.0
13	0.0	0.0	0.0	4.977408785873118E-4	0.0
14	0.0	0.0	0.0	4.977408785873118E-4	1.2443521964682796E-4
15	0.0	0.0	0.0	6.221760982341398E-4	1.2443521964682796E-4
16	0.0	0.0	0.0	6.221760982341398E-4	1.2443521964682796E-4
17	0.0	0.0	0.0	7.466113178809677E-4	1.2443521964682796E-4
18	0.0	0.0	0.0	8.710465375277957E-4	1.2443521964682796E-4
19	0.0	0.0	0.0	8.710465375277957E-4	1.2443521964682796E-4
20	0.0	0.0	0.0	0.0011199169768214517	1.2443521964682796E-4
21	0.0	1.2443521964682796E-4	0.0	0.0012443521964682796	1.2443521964682796E-4
22	0.0	1.2443521964682796E-4	1.2443521964682796E-4	0.0022398339536429035	1.2443521964682796E-4
23	0.0	1.2443521964682796E-4	1.2443521964682796E-4	0.004479667907285807	1.2443521964682796E-4
24	0.0	1.2443521964682796E-4	1.2443521964682796E-4	0.007092807519869193	1.2443521964682796E-4
25	0.0	1.2443521964682796E-4	1.2443521964682796E-4	0.007839418837750162	1.2443521964682796E-4
26	0.0	1.2443521964682796E-4	1.2443521964682796E-4	0.009581511912805754	1.2443521964682796E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATC	30	8.4125437E-4	26.667809	17
TAGGACC	875	0.0	23.036686	4
GACCTAG	65	3.8165672E-7	22.152037	7
GGTTTCG	80	5.2477844E-9	21.999573	20
TGCAGTA	40	0.0044796034	20.0021	2
AGGACCT	1700	0.0	19.385447	5
TCGGGGT	85	2.394263E-7	18.823164	24
ATGTACG	95	3.7860445E-8	18.525955	23
ATTTAGA	885	0.0	18.454098	1
GGACCTG	1695	0.0	18.215668	6
TCCTATA	185	0.0	18.16407	2
CTAGGAC	150	0.0	18.131851	3
ACTGTTC	480	0.0	17.998531	8
TAGAAAT	930	0.0	17.889898	4
TTCGGGG	90	4.3635737E-7	17.777433	23
GTACGAA	90	4.3635737E-7	17.777433	25
ATAGGAC	225	0.0	17.776327	3
CGCCCTT	45	0.008855359	17.77522	6
TTTAGAA	960	0.0	17.668524	2
GTCCTAA	1895	0.0	17.65928	1
>>END_MODULE