FastQCFastQC Report
Thu 2 Feb 2017
SRR4062936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803631
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT81601.0153913923181161No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT56680.7052988249582208No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA42780.53233386964913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT34500.42930150778155646No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA33700.4193466902098102No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG33080.4116317065917069No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC30130.37492331679589264No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.3143233648278874No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA19760.24588399402213204No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT17690.22012590355523867No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA17600.21900598657841722No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA14430.17956002195037277No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA14310.1780667993146108No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA14120.17570253014132106No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA14050.17483148360379328No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA12810.15940151636758662No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA12340.1535530610441857No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC11900.14807791137972526No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA11630.14471816044926092No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA11420.14210502083667753No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG10960.13638100073292345No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT10780.13414116677928054No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG10500.13065698062916936No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10380.12916375799340743No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA10130.1260528775022367No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT10000.12443521964682795No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT9580.11920894042166119No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA9540.11871119954307387No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT9360.11647136558943097No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT9170.11410709641614125No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.10900525241062128No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA8670.10788533543379984No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG8630.10738759455521253No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG8450.10514776060156963No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG8450.10514776060156963No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC8410.10465001972298231No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT8380.10427671406404183No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA8370.10415227884439501No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA8360.10402784362474816No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT8350.10390340840510134No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT8260.1027834914282799No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA8110.10091696313357748No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC8070.10041922225499016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT404.9551836E-628.0022877
ACGTGTA404.9551836E-628.0022878
TTCACGG356.430154E-527.42739926
TGCACCG308.4106345E-426.6688465
CTAGGTC350.002065769222.8575864
TAGGACC9550.021.4454444
ATTCTAG801.2640521E-720.000391
GTCGAGA507.192422E-419.2003719
CACGGTT507.195434E-419.19917928
ACATACG507.195434E-419.19917930
TTTAGAA8350.019.1608582
AGGCGCT601.1369283E-418.6681927
CGTGTAA601.1369283E-418.6681929
AGGACAA1055.9626473E-918.2872075
ATAGGAC2100.018.286073
CCCTATA701.785872E-518.286071
TTAGAAA8050.018.286073
AGGACCT17050.018.1129175
AGGCGGG1159.404175E-1018.08618427
ATTTAGA8200.017.9515691
Produced by FastQC (version 0.11.5)