FastQCFastQC Report
Thu 2 Feb 2017
SRR4062935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2643613
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40970.15497729811436092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32500.020.7514021
GGTATCA11100.019.4831641
ATAACGA3600.017.7772112
ACGAACG3800.016.4211515
CGATAAC3850.016.20696810
GTATTAT2400.016.019491
TAACGAA4000.016.00009513
GATAACG4050.015.80196511
CCGATAA3950.015.7966669
TAGGACG6400.015.4977464
CGAACGA4150.015.03594916
TCCGATA4300.014.8824028
AGGACGT7200.014.6642575
GTCCTAA6000.014.41754151
TCAACGC45950.014.2743114
TATCAAC46900.014.1973522
TTCCGAT4650.014.1062767
AACGCAG46800.014.0831566
AACGAAC4550.014.06601614
ATCAACG46700.014.046663
Produced by FastQC (version 0.11.5)